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1.
Protein Cell ; 2024 Apr 18.
Artículo en Inglés | MEDLINE | ID: mdl-38635907

RESUMEN

Scavenger receptor class B, member 2 (SCARB2) is linked to Gaucher disease (GD) and Parkinson's disease (PD). Deficiency in the SCARB2 gene causes progressive myoclonus epilepsy (PME), a rare group of inherited neurodegenerative diseases characterized by myoclonus. We found that Scarb2 deficiency in mice leads to age-dependent dietary lipid malabsorption, accompanied with vitamin E deficiency. Our investigation revealed that Scarb2 deficiency is associated with gut dysbiosis and an altered bile acid pool, leading to hyperactivation of FXR in intestine. Hyperactivation of FXR impairs epithelium renewal and lipid absorption. Patients with SCARB2 mutations have a severe reduction in their vitamin E levels and cannot absorb dietary vitamin E. Finally, inhibiting FXR or supplementing vitamin E ameliorates the neuromotor impairment and neuropathy in Scarb2 knockout mice. These data indicate that gastrointestinal dysfunction is associated with SCARB2 deficiency-related neurodegeneration, and SCARB2-associated neurodegeneration can be improved by addressing the nutrition deficits and gastrointestinal issues.

2.
Molecules ; 28(11)2023 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-37298973

RESUMEN

The specificity of the tumor microenvironment (TME) severely limits the effectiveness of tumor treatment. In this study, we prepared a composite nanoparticle of manganese dioxide and selenite by a one-step redox method, and their stability under physiological conditions was improved with a bovine serum protein modification to obtain MnO2/Se-BSA nanoparticles (SMB NPs). In the SMB NPs, manganese dioxide and selenite endowed the SMB NPs with acid-responsive and catalytic, and antioxidant properties, respectively. The weak acid response, catalytic activity, and antioxidant properties of composite nanoparticles were verified experimentally. Moreover, in an in vitro hemolysis assay, different concentrations of nanoparticles were incubated with mouse erythrocytes, and the hemolysis ratio was less than 5%. In the cell safety assay, the cell survival ratio was as high as 95.97% after the co-culture with L929 cells at different concentrations for 24 h. In addition, the good biosafety of composite nanoparticles was verified at the animal level. Thus, this study helps to design high-performance and comprehensive therapeutic reagents that are responsive to the hypoxia, weak acidity, hydrogen peroxide overexpression nature of TME and overcome the limitations of TME.


Asunto(s)
Nanopartículas , Selenio , Ratones , Animales , Óxidos , Compuestos de Manganeso/farmacología , Manganeso/farmacología , Antioxidantes/farmacología , Hemólisis , Peróxido de Hidrógeno/metabolismo , Microambiente Tumoral , Línea Celular Tumoral
3.
Biomater Sci ; 11(7): 2486-2503, 2023 Mar 28.
Artículo en Inglés | MEDLINE | ID: mdl-36779258

RESUMEN

Photothermal therapy (PTT) is a promising approach for treating cancer. However, it suffers from the formation of local lesions and subsequent bacterial infection in the damaged area. To overcome these challenges, the strategy of mild PTT following the high-temperature ablation of tumors is studied to achieve combined tumor suppression, wound healing, and bacterial eradication using a hydrogel. Herein, Bi2S3 nanorods (NRs) are employed as a photothermal agent and coated with hyaluronic acid to obtain BiH NRs with high colloidal stability. These NRs and allantoin are loaded into an injectable Fe3+-coordinated hydrogel composed of sodium alginate (Alg) and Farsi gum (FG), which is extracted from Amygdalus scoparia Spach. The hydrogel can be used for localized cancer therapy by high-temperature PTT, followed by wound repair through the combination of mild hyperthermia and allantoin-mediated induction of cell proliferation. In addition, an outstanding blood clotting effect is observed due to the water-absorbing ability and negative charge of FG and Alg as well as the porous structure of hydrogels. The hydrogels also eradicate infection owing to the local heat generation and intrinsic antimicrobial activity of the NRs. Lastly, in vivo studies reveal an efficient photothermal-based tumor eradication and accelerated wound healing by the hydrogel.


Asunto(s)
Hipertermia Inducida , Neoplasias , Humanos , Hidrogeles/química , Alantoína , Calefacción , Cicatrización de Heridas , Neoplasias/tratamiento farmacológico , Metales , Antibacterianos/farmacología , Antibacterianos/química
4.
Colloids Surf B Biointerfaces ; 221: 113010, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36375292

RESUMEN

The degradation of extracellular matrix (ECM) to increase drug permeability is an attractive approach to enhancing pancreatic cancer therapy efficiency. Herein, polypyrrole nanoparticles (PPy NPs) were prepared by a template-guided chemical oxidation method. These PPy NPs with abundant surface pores were used to load the anticancer drug doxorubicin (DOX). In order to intelligently control the DOX release, PPy/DOX NPs were further entrapped with a thermoresponsive ligand, lauric acid (LA), to form PPy-LA/DOX NPs. Bromelain (BL) was then grafted onto the surface of PPy-LA NPs or PPy-LA/DOX NPs through an amidation reaction with the carboxyl group of LA. It was found that the DOX release of PPy-LA/DOX NPs was pH and temperature responsive, reaching a maximum amount of 85.9% within 48 h at pH = 5.4 and 50 °C. Moreover, it was demonstrated that the resultant PLB (PPy-LA-BL) NPs could efficiently hydrolyze the collagen in ECM and enhance the permeability of DOX to the pancreatic tumor. Remarkably, PLB NPs not only featured admirable photothermal conversion but also exhibited obvious photoacoustic imaging capability, which enabled imaging-guided enhanced tumor ablation. This study is anticipated to provide a feasible strategy to improve the permeability of nanoparticles to tumors.


Asunto(s)
Neoplasias Pancreáticas , Polímeros , Humanos , Pirroles , Doxorrubicina/farmacología , Neoplasias Pancreáticas/tratamiento farmacológico , Matriz Extracelular
5.
Int J Biol Macromol ; 228: 111-122, 2023 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-36563819

RESUMEN

In this study, a methacrylic gelatin/oxidized dextran/montmorillonite­strontium/polypyrrole (GOMP) hydrogel was prepared. The GOMP hydrogel had dual network structure which was formed through photoinitiator-initiated double bond polymerization and Schiff base reaction. The network structure led to a sustained release of the antitumor drug, doxorubicin (DOX). Polypyrrole introduced the conductivity and high photothermal conversion capacity to the GOMP hydrogel, which showed a photothermal conversion efficiency of 31.61 % under 808 nm laser radiation. The GOMP hydrogel had good swelling properties in solvents. Further study showed that the GOMP hydrogel had good biocompatibility and excellent biodegradability in vitro and in vivo. The experiments of in vitro tumor therapy and in vivo anti-tumor recurrence indicated that the DOX-loaded GOMP hydrogel had synergistic effects on tumor cell apoptosis based on chemotherapy and photothermal therapy. In addition, montmorillonite­strontium (MMT-Sr) doped in the hydrogel not only improved the mechanical properties of the hydrogel but also promoted potential bone regeneration. The multifunctional DOX-loaded GOMP hydrogel with bone regeneration, photothermal therapy, and chemotherapy functions has great potential application for treating osteosarcoma.


Asunto(s)
Neoplasias Óseas , Hipertermia Inducida , Osteosarcoma , Humanos , Polímeros/química , Gelatina/farmacología , Hidrogeles/química , Bentonita/farmacología , Línea Celular Tumoral , Fototerapia , Pirroles , Recurrencia Local de Neoplasia , Osteosarcoma/tratamiento farmacológico , Doxorrubicina/farmacología , Doxorrubicina/química , Regeneración Ósea , Neoplasias Óseas/tratamiento farmacológico
6.
Adv Healthc Mater ; 11(15): e2200524, 2022 08.
Artículo en Inglés | MEDLINE | ID: mdl-35611682

RESUMEN

In this study, a polyvinylpyrrolidone (PVP)-decorated MoSe2 (MoSe2 -PVP) nanoparticle with excellent photothermal transforming ability and chlorin E6 (Ce6) loading capacity is designed for combined tumor photothermal therapy (PTT), tumor photodynamic therapy (PDT), and immunotherapy. The light-to-heat conversion efficiency under irradiation with an 808 nm near-infrared laser is as high as 59.28%. The MoSe2 -PVP NPs could function as an artificial catalase and catalyze the decomposition of H2 O2 . Their catalytic activity and thermal durability are higher than the native catalase, which relieve the tumor hypoxia status and sensitize the tumor PDT. The data show that the synthetic MoSe2 -PVP is biodegradable, owing to the oxidation of the Mo4+ to Mo6+ . Moreover, its degradation products could increase the proportion of mature dendritic cells and CD8+ thymus (T) cells and promote the infiltration of active CD8+ T cells in tumors. The immune checkpoint inhibitor, programmed cell death protein 1 monoclonal antibody is combined with MoSe2 -PVP and it is found that its degradation product could efficiently change the immune microenvironment of the tumor.


Asunto(s)
Nanopartículas , Neoplasias , Fotoquimioterapia , Porfirinas , Linfocitos T CD8-positivos , Catalasa , Línea Celular Tumoral , Humanos , Inmunoterapia , Molibdeno , Neoplasias/tratamiento farmacológico , Fármacos Fotosensibilizantes/farmacología , Fármacos Fotosensibilizantes/uso terapéutico , Porfirinas/farmacología , Porfirinas/uso terapéutico , Microambiente Tumoral
7.
J Nanobiotechnology ; 19(1): 203, 2021 Jul 07.
Artículo en Inglés | MEDLINE | ID: mdl-34233696

RESUMEN

The combination of photothermal therapy (PTT) and photodynamic therapy (PDT) has attracted attention due to its enhanced tumor therapy effect. This study proposes a novel nanoenzyme-based theranostic nanoplatform, IrO2@MSN@PDA-BSA(Ce6), for the combined PTT and PDT of tumors. IrO2 was prepared by a simple hydrolysis method and coated with a thin layer of mesoporous silica (MSN) to facilitate the physical adsorption of Chlorin e6 (Ce6). The PDA coating and IrO2 NPs of the nanoplatform demonstrated an improved photothermal conversion efficiency of 29.8% under NIR irradiation. Further, the Ce6 loading imparts materials with the ability to produce reactive oxygen species (ROS) under 660 nm NIR laser irradiation. It was also proved that the IrO2 NPs could catalyze the hydrogen peroxide (H2O2) in the tumor microenvironment (TME) to generate endogenous oxygen (O2), thereby enhancing the efficiency of PDT. The in vitro and in vivo experiments indicated that the nanocomposite was highly biocompatible and could produce a satisfactory tumor therapeutic effect. Thus, the findings of the present study demonstrate the viability of using theranostic nanoenzymes for translational medicine.


Asunto(s)
Catalasa/metabolismo , Iridio/química , Nanocompuestos/química , Nanopartículas/química , Fotoquimioterapia/métodos , Animales , Materiales Biocompatibles , Línea Celular Tumoral , Clorofilidas , Femenino , Peróxido de Hidrógeno , Luz , Ratones , Neoplasias/tratamiento farmacológico , Oxígeno , Porfirinas , Dióxido de Silicio , Microambiente Tumoral
8.
Colloids Surf B Biointerfaces ; 205: 111852, 2021 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-34030106

RESUMEN

In this research, MnO2/IrO2 nanoplatform was one-step synthesized from the heat-induced oxidation-reduction between potassium permanganate and iridium chloride and modified with polyvinylpyrrolidone (PVP) on the surface to obtain MnO2/IrO2-PVP nanoparticles (MIP NPs) with excellent colloidal stability of biocompatibility. Then, the photosensitizer Chlorin e6 (Ce6) was loaded onto the surface of MIP NPs. The IrO2 can efficiently transform the 808 nm near-infrared laser into heat with a photothermal conversion of 27.57 % for tumor photothermal therapy. Interestingly, the MnO2 can not only react with the redundant H+ and realize the magnetic resonance imaging of the tumor but also catalytic the decomposition of H2O2 in the tumor to generate O2 and relieve the hypoxia status of the tumor. The in-situ formed O2 can promote the production of cancer cell-toxic singlet oxygens (1O2) under the irradiation of 660 nm laser and boost the tumor photodynamic therapy efficiency. Moreover, it was found that PVP can fall off from the MIP NPs to increase their accumulation in the tumor. Such a MIP/Ce6-based nanoplatform which plays the synergism with tumor microenvironment shows promising potential for the combined photo-therapy of the tumor.


Asunto(s)
Nanopartículas , Neoplasias , Fotoquimioterapia , Línea Celular Tumoral , Humanos , Peróxido de Hidrógeno , Compuestos de Manganeso , Neoplasias/tratamiento farmacológico , Óxidos , Fármacos Fotosensibilizantes/uso terapéutico , Povidona , Microambiente Tumoral
9.
J Nanobiotechnology ; 19(1): 36, 2021 Feb 03.
Artículo en Inglés | MEDLINE | ID: mdl-33536031

RESUMEN

Owing to the hypoxia status of the tumor, the reactive oxygen species (ROS) production during photodynamic therapy (PDT) of the tumor is less efficient. Herein, a facile method which involves the synthesis of Mg-Mn-Al layered double hydroxides (LDH) clay with MoS2 doping in the surface and anionic layer space of LDH was presented, to integrate the photo-thermal effect of MoS2 and imaging and catalytic functions of Mg-Mn-Al LDH. The designed LDH-MoS2 (LMM) clay composite was further surface-coated with bovine serum albumin (BSA) to maintain the colloidal stability of LMM in physiological environment. A photosensitizer, chlorin e6 (Ce6), was absorbed at the surface and anionic layer space of LMM@BSA. In the LMM formulation, the magnetic resonance imaging of Mg-Mn-Al LDH was enhanced thanks to the reduced and acid microenvironment of the tumor. Notably, the ROS production and PDT efficiency of Ce6 were significantly improved, because LMM@BSA could catalyze the decomposing of the overexpressed H2O2 in tumors to produce oxygen. The biocompatible LMM@BSA that played the synergism with tumor microenvironment is a promising candidate for the effective treatment of cancer.


Asunto(s)
Catalasa/uso terapéutico , Disulfuros/uso terapéutico , Molibdeno/uso terapéutico , Nanoestructuras/uso terapéutico , Neoplasias/terapia , Fármacos Fotosensibilizantes/uso terapéutico , Porfirinas/uso terapéutico , Animales , Materiales Biomiméticos/síntesis química , Materiales Biomiméticos/uso terapéutico , Clorofilidas , Células HT29 , Humanos , Hidróxidos/uso terapéutico , Imagen por Resonancia Magnética/métodos , Ratones , Neoplasias/diagnóstico por imagen , Neoplasias/metabolismo , Fotoquimioterapia/métodos , Terapia Fototérmica/métodos , Especies Reactivas de Oxígeno/metabolismo , Nanomedicina Teranóstica/métodos
10.
Colloids Surf B Biointerfaces ; 200: 111591, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33548893

RESUMEN

Radiotherapy (RT) is becoming a pervasive therapeutic pattern in clinical cancer therapy. However, the hypoxic microenvironment of tumors has a strong resistance to radiotherapy and could lead to a potential recurrence and metastasis after the treatment. Therefore, the use of synergistic strategies for improving and supplementing the RT efficiency is important. Herein, a novel Bi2S3/alginate (ALG) hydrogel containing tirapazamine (TPZ) was designed for the effective suppression of tumor recurrence, by introducing Bi3+ into the ALG, Na2S and TPZ solution. In this formulation, Bi3+ was used to crosslink with the ALG to form the hydrogel and react with S2- to simultaneously form Bi2S3 nanoparticles in the hydrogel matrix. The resulting Bi2S3 nanoparticles not only exhibit the superb radiosensitization effect to boost the effective eradication of tumors during RT but also manifest an excellent photothermal transforming performance for tumor hyperthermia and computed tomography (CT) imaging capacity for tumor monitoring. Furthermore, the RT caused hypoxia could activate the reductive prodrug TPZ and enhance its therapeutic efficiency. The reported hydrogel system provides an efficient and safe therapeutic strategy for current local tumor therapy.


Asunto(s)
Hipertermia Inducida , Neoplasias , Profármacos , Línea Celular Tumoral , Humanos , Hidrogeles , Neoplasias/tratamiento farmacológico , Tirapazamina , Microambiente Tumoral
11.
Chembiochem ; 21(6): 865-873, 2020 03 16.
Artículo en Inglés | MEDLINE | ID: mdl-31613042

RESUMEN

Polymeric hydrogels with three-dimensional network structures have found tremendous applications in biomedicine. Herein, we report the synthesis of a multifunctional implant based on ovalbumin (OVA) as a carrier capable of synergistically delivering a photothermal transducing agent (polydopamine, PDA) to tumors. The formation of PDA was achieved by utilizing the basicity of OVA, whereas the formation of the hydrogel implant was achieved through the in vitro/in vivo near-infrared (NIR) laser-induced hyperthermia of PDA. The as-prepared PDA@OVA implant exhibits high photothermal conversion efficiency (38.7 %). Once implanted in vivo, the OVA-based implant shows great versatility in the treatment of malignant tumors. Furthermore, a chemotherapeutic (doxorubicin, DOX) and a contrast agent (iohexol), dispersed in the OVA solution in advance, can also be firmly entrapped in the hydrogel along with the hydrogel formation. It is anticipated that the multifunctional OVA-based implant, not showing any obvious toxicity to healthy tissue, could be a promising system for synergistic cancer treatment.


Asunto(s)
Antibióticos Antineoplásicos/farmacología , Doxorrubicina/farmacología , Hipertermia Inducida , Indoles/farmacología , Yohexol/farmacología , Ovalbúmina/química , Fármacos Fotosensibilizantes/farmacología , Terapia Fototérmica , Polímeros/farmacología , Animales , Antibióticos Antineoplásicos/administración & dosificación , Línea Celular , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Doxorrubicina/administración & dosificación , Portadores de Fármacos/administración & dosificación , Portadores de Fármacos/química , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Indoles/administración & dosificación , Rayos Infrarrojos , Yohexol/administración & dosificación , Ratones , Ratones Endogámicos , Neoplasias Experimentales/diagnóstico por imagen , Neoplasias Experimentales/tratamiento farmacológico , Imagen Óptica , Ovalbúmina/administración & dosificación , Fármacos Fotosensibilizantes/administración & dosificación , Polímeros/administración & dosificación , Microambiente Tumoral/efectos de los fármacos
12.
Soft Matter ; 16(1): 132-141, 2020 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-31774105

RESUMEN

In this study, sodium alginate (ALG)/poly dopamine (PDA)-polyvinylpyrrolidone (PVP) nanocomposites was synthesized via a one-step electrostatic spraying method. The spinning solution of ALG and dopamine was electrostatically sprayed into an alkaline solution of PVP, calcium chloride and tris buffer (pH = 8.5), in which the gelation of ALG and the polymerization of dopamine could be simultaneously triggered. PDA hence produced possesses a high photothermal conversion efficiency, while the PVP that was facilely conjugated onto the surface of nanocomposites improves the colloidal stability and compatibility of the material. Moreover, the ALG renders the nanocomposite excellent drug (doxorubicine, DOX) loading capacity. Promisingly, the temperature increment during the PTT process could promote the DOX release, thus enhancing its therapeutic effect. The in vitro/in vivo biosafety and tumor treatment experiments further corroborate that the ALG/PDA-PVP nanocomposites have remarkable biocompatibility and synergism for tumor hyperthermia and chemotherapy. Consequently, such a one-step electrospray strategy provides a new way for designing nanomaterials and is expected to significantly promote the development of organic photothermal therapeutic agents with excellent bio-compatibility.


Asunto(s)
Alginatos/química , Dopamina/química , Nanocompuestos/química , Neoplasias/tratamiento farmacológico , Povidona/química , Animales , Antineoplásicos/química , Antineoplásicos/metabolismo , Antineoplásicos/farmacología , Antineoplásicos/uso terapéutico , Materiales Biocompatibles/química , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Doxorrubicina/química , Doxorrubicina/metabolismo , Doxorrubicina/farmacología , Doxorrubicina/uso terapéutico , Portadores de Fármacos/química , Liberación de Fármacos , Humanos , Hipertermia Inducida , Rayos Infrarrojos , Ratones , Neoplasias/patología , Neoplasias/terapia , Fototerapia , Distribución Tisular
13.
Carbohydr Polym ; 222: 115039, 2019 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-31320053

RESUMEN

The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy. Chitosan (CS) solution was injected into the tumor at room temperature and automatically gelled after warming to body temperature in the present of ß-glycerophosphate (ß-GP). Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells. More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL). Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation. Moreover, the chitosan-based hydrogel had antibacterial effects. The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.


Asunto(s)
Antineoplásicos/uso terapéutico , Quitosano/química , Neoplasias del Colon/tratamiento farmacológico , Doxorrubicina/uso terapéutico , Portadores de Fármacos/química , Hidrogeles/química , Animales , Antibacterianos/administración & dosificación , Antibacterianos/química , Antibacterianos/farmacología , Antineoplásicos/administración & dosificación , Bismuto/administración & dosificación , Línea Celular , Quitosano/administración & dosificación , Quitosano/farmacología , Disulfuros/administración & dosificación , Disulfuros/efectos de la radiación , Doxorrubicina/administración & dosificación , Portadores de Fármacos/administración & dosificación , Portadores de Fármacos/farmacología , Liberación de Fármacos , Escherichia coli/efectos de los fármacos , Hidrogeles/administración & dosificación , Hidrogeles/farmacología , Hipertermia Inducida/métodos , Rayos Infrarrojos , Inyecciones , Ratones Endogámicos BALB C , Molibdeno/administración & dosificación , Molibdeno/efectos de la radiación , Polietilenglicoles/administración & dosificación , Polietilenglicoles/química , Sulfuros/administración & dosificación , Sulfuros/efectos de la radiación , Temperatura , Ensayos Antitumor por Modelo de Xenoinjerto
14.
Int J Nanomedicine ; 14: 3893-3909, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31239663

RESUMEN

Background: Photothermal and chemotherapy treatment has been frequently studied for cancer therapy; however, chemotherapy is equally toxic to both normal and cancer cells. The clinical application value of most kinds of photothermal transforming agents remains limited, due to their poor degradation and minimal accumulation in tumors. Materials and methods: We reported the synthesis of photothermal transforming agents (MoS2) and chemotherapeutic (doxorubicin, DOX) co-loaded electrospun nanofibers using blend electrospinning for the treatment of postoperative tumor recurrence. Results: Under the irradiation of an 808 nm laser, the as-prepared chitosan/polyvinyl alcohol/MoS2/DOX nanofibers showed an admirable photothermal conversion capability with a photothermal conversion efficiency of 23.2%. These composite nanofibers are in vitro and in vivo biocompatible. In addition, they could control the sustained release of DOX and the generated heat can sensitize the chemotherapeutic efficacy of DOX via enhancing its release rate. Their chemo-/photothermal combined therapy efficiency was systematically studied in vitro and in vivo. Instead of circulating with the body fluid, MoS2 was trapped by the nanofibrous matrix in the tumor and so its tumor-killing ability was not compromised, thus rendering this composite nanofiber a promising alternative for future clinical translation within biomedical application fields. Conclusion: Chitosan/polyvinyl alcohol/MoS2/DOX nanofibers showed an excellent photothermal conversion capability with a photothermal conversion efficiency of 23.2% and can completely inhibit the postoperative tumor reoccurrence.


Asunto(s)
Disulfuros/química , Doxorrubicina/uso terapéutico , Molibdeno/química , Nanofibras/química , Nanotecnología/métodos , Neoplasias/terapia , Fototerapia , Animales , Materiales Biocompatibles/farmacología , Muerte Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Reactivos de Enlaces Cruzados/química , Doxorrubicina/farmacología , Liberación de Fármacos , Células HT29 , Humanos , Ratones Endogámicos BALB C , Ratones Desnudos , Nanofibras/ultraestructura , Recurrencia Local de Neoplasia/patología , Neoplasias/sangre , Neoplasias/patología , Neoplasias/cirugía , Estándares de Referencia , Resultado del Tratamiento
15.
Colloids Surf B Biointerfaces ; 177: 346-355, 2019 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-30772669

RESUMEN

With an excellent near-infrared (NIR) light-responsive property, polypyrrole (PPy) nanoparticle has emerged as a promising NIR photothermal transducing agent for tumor photothermal therapy (PTT). Herein, we reported the PVP mediated one-pot synthesis of colloidal stable and biocompatible PPy nanoparticles (PPy-PVP NPs) for combined tumor photothermal-chemotherapy. The influence of molecular weight and PVP concentration on the spectroscopic characteristic, photothermal feature, drug loading performance, and antitumor efficiency of the resultant PPy-PVP NPs was systematically studied. By choosing PVP with a molecular weight of 360 kDa (concentration of 5 mg/mL) as the template and surface modifier during the synthesis, PPy-PVP NPs with optimal spectroscopic characteristic, photothermal feature, drug loading performance, and antitumor efficiency were synthesized. Findings in this study are anticipated to provide an in-depth understanding of the important character of surface engineering in the rational design and biomedical applications of PPy NPs.


Asunto(s)
Antineoplásicos/farmacología , Materiales Biocompatibles/farmacología , Nanopartículas/química , Fototerapia , Polímeros/farmacología , Pirroles/farmacología , Animales , Antineoplásicos/síntesis química , Antineoplásicos/química , Materiales Biocompatibles/síntesis química , Materiales Biocompatibles/química , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Coloides/química , Coloides/farmacología , Liberación de Fármacos , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Rayos Infrarrojos , Ratones , Ratones Endogámicos , Tamaño de la Partícula , Polímeros/síntesis química , Polímeros/química , Pirroles/síntesis química , Pirroles/química , Propiedades de Superficie , Células Tumorales Cultivadas
16.
Macromol Biosci ; 18(10): e1800206, 2018 10.
Artículo en Inglés | MEDLINE | ID: mdl-30188003

RESUMEN

In this work, a novel kind of electrospun microfiber to deliver a photothermal agent and an anticancer drug to tumor sites is explored. Photothermal therapy agent (MoS2 nanosheets) and doxorubicin (DOX) are incorporated with poly(lactic-co-glycolic acid) (PLGA) microfiber via electrospinning a solution of PLGA, MoS2 , and DOX. The designed microfiber with uniform fibrous morphology and negligible in vitro/in vivo hemo-/histo-toxicity is used as a durable photothermal agent, which shows an excellent photothermal transform ability and acceptable photothermal stability in both the first and second near-infrared light (NIR I and II) biowindows. The synergistic in vivo tumor chemotherapy and photothermal therapy efficiency of the composite microfibers are studied in postoperative treatment of cancer. It is found that the tumor postoperative reoccurrence can be completely prohibited owing to the synergistic tumor therapy efficiency in both the NIR I and NIR II biowindows.


Asunto(s)
Doxorrubicina , Sistemas de Liberación de Medicamentos/métodos , Hipertermia Inducida , Rayos Infrarrojos , Neoplasias Experimentales , Fototerapia , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Animales , Línea Celular Tumoral , Doxorrubicina/química , Doxorrubicina/farmacocinética , Doxorrubicina/farmacología , Ratones , Neoplasias Experimentales/metabolismo , Neoplasias Experimentales/patología , Neoplasias Experimentales/terapia , Copolímero de Ácido Poliláctico-Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico-Ácido Poliglicólico/farmacocinética , Copolímero de Ácido Poliláctico-Ácido Poliglicólico/farmacología
17.
ACS Appl Mater Interfaces ; 10(4): 3392-3404, 2018 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-29313334

RESUMEN

The objective of the present study was to construct an alginate (AG)-based phase-changeable and injectable hydrogel for imaging-guided tumor hyperthermia and chemotherapy. Based on the binding between the α-l-guluronic blocks of AG and calcium ions, the AG/MoS2/Bi2S3-poly(ethylene glycol) (MBP)/doxorubicin (DOX) solution formed a cross-linked hydrogel to simultaneously encapsulate MBP nanosheets and DOX within the hydrogel matrix. The in situ formed hydrogel can act as a reservoir to control the release of entrapped drug molecules, and the doped MBP nanosheets and DOX can realize computed tomography/photoacoustic dual-modal imaging-guided in vivo tumor photothermal therapy and chemotherapy, respectively. The AG/MBP/DOX hydrogel exhibited excellent photothermal conversion properties with mass extinction coefficient of 45.1 L/g/cm and photothermal conversion efficiency of 42.7%. Besides, the heat from the photothermal transformation of MBP can promote drug diffusion from the hydrogel to realize on-demand drug release. Additionally, the hydrogel system can restrain MBP and DOX from entering into the blood stream during therapy, and therefore substantially decrease their side effects on normal organs. More importantly, the drug loading of the AG hydrogel was general and can be extended to the encapsulation of antibiotics, such as amoxicillin, for the prevention of postoperative infections.


Asunto(s)
Ácido Algínico/química , Doxorrubicina , Humanos , Hidrogeles , Hipertermia Inducida , Neoplasias
18.
Adv Colloid Interface Sci ; 249: 374-385, 2017 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-28335985

RESUMEN

The design and development of multifunctional nanoplatforms for biomedical applications still remains to be challenging. This review reports the recent advances in aqueous-phase synthesis of iron oxide nanoparticles (Fe3O4 NPs) and their composites for magnetic resonance (MR) imaging and photothermal therapy of cancer. Water dispersible and colloidally stable Fe3O4 NPs synthesized via controlled coprecipitation route, hydrothermal route and mild reduction route are introduced. Some of key strategies to improve the r2 relaxivity of Fe3O4 NPs and to enhance their uptake by cancer cells are discussed in detail. These aqueous-phase synthetic methods can also be applied to prepare Fe3O4 NP-based composites for dual-mode molecular imaging applications. More interestingly, aqueous-phase synthesized Fe3O4 NPs are able to be fabricated as multifunctional theranostic agents for multi-mode imaging and photothermal therapy of cancer. This review will provide some meaningful information for the design and development of various Fe3O4 NP-based multifunctional nanoplatforms for cancer diagnosis and therapy.


Asunto(s)
Medios de Contraste/síntesis química , Óxido Ferrosoférrico/síntesis química , Nanopartículas de Magnetita/química , Neoplasias Glandulares y Epiteliales/terapia , Nanomedicina Teranóstica/métodos , Animales , Línea Celular Tumoral , Técnicas de Química Sintética , Medios de Contraste/administración & dosificación , Óxido Ferrosoférrico/administración & dosificación , Células HeLa , Células Hep G2 , Humanos , Hipertermia Inducida/métodos , Inyecciones Intralesiones , Terapia por Luz de Baja Intensidad/métodos , Nanopartículas de Magnetita/administración & dosificación , Ratones , Neoplasias Glandulares y Epiteliales/metabolismo , Neoplasias Glandulares y Epiteliales/patología , Agua/química , Ensayos Antitumor por Modelo de Xenoinjerto
19.
Sci Rep ; 6: 28325, 2016 06 21.
Artículo en Inglés | MEDLINE | ID: mdl-27325015

RESUMEN

We herein report the development of multifunctional folic acid (FA)-targeted Fe3O4 @ Au nanostars (NSs) for targeted multi-mode magnetic resonance (MR)/computed tomography (CT)/photoacoustic (PA) imaging and photothermal therapy (PTT) of tumors. In this present work, citric acid-stabilized Fe3O4/Ag composite nanoparticles prepared by a mild reduction route were utilized as seeds and exposed to the Au growth solution to induce the formation of Fe3O4 @ Au core/shell NSs. Followed by successive decoration of thiolated polyethyleneimine (PEI-SH), FA via a polyethylene glycol spacer, and acetylation of the residual PEI amines, multifunctional Fe3O4 @ Au NSs were formed. The designed multifunctional NSs possess excellent colloidal stability, good cytocompatibility in a given concentration range, and specific recognition to cancer cells overexpressing FA receptors. Due to co-existence of Fe3O4 core and star-shaped Au shell, the NSs can be used for MR and CT imaging of tumors, respectively. Likewise, the near infrared plasmonic absorption feature also enables the NSs to be used for PA imaging and PTT of tumors. Our study clearly demonstrates a unique theranostic nanoplatform that can be used for high performance multi-mode imaging-guided PTT of tumors, which may be extendable for theranostics of different diseases in translational medicine.


Asunto(s)
Medios de Contraste/química , Nanopartículas/química , Neoplasias/diagnóstico por imagen , Animales , Medios de Contraste/administración & dosificación , Óxido Ferrosoférrico/química , Ácido Fólico/química , Oro/química , Células HeLa , Humanos , Terapia por Láser , Imagen por Resonancia Magnética , Ratones , Nanopartículas/administración & dosificación , Neoplasias/terapia , Fototerapia , Nanomedicina Teranóstica , Tomografía Computarizada por Rayos X , Ensayos Antitumor por Modelo de Xenoinjerto
20.
Int J Nanomedicine ; 11: 1819-33, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27199557

RESUMEN

Two-dimensional MoS2 nanosheet has been extensively explored as a photothermal agent for tumor regression; however, its surface modification remains a great challenge. Herein, as an alternative to surface polyethylene glycol modification (PEGylation), a facile approach based on "thin-film" strategy has been proposed for the first time to produce soybean phospholipid-encapsulated MoS2 (SP-MoS2) nanosheets. By simply vacuum-treating MoS2 nanosheets/soybean phospholipid/chloroform dispersion in a rotary evaporator, SP-MoS2 nanosheet was successfully constructed. Owing to the steric hindrance of polymer chains, the surface-coated soybean phospholipid endowed MoS2 nanosheets with excellent colloidal stability. Without showing detectable in vitro and in vivo hemolysis, coagulation, and cyto-/histotoxicity, the constructed SP-MoS2 nanosheets showed good photothermal conversion performance and photothermal stability. SP-MoS2 nanosheet was shown to be a promising platform for in vitro and in vivo breast tumor photothermal therapy. The produced SP-MoS2 nanosheets featured low cost, simple fabrication, and good in vivo hemo-/histocompatibility and hold promising potential for future clinical tumor therapy.


Asunto(s)
Disulfuros/síntesis química , Glycine max/química , Hipertermia Inducida/métodos , Neoplasias Mamarias Animales/terapia , Nanopartículas/química , Fosfolípidos/química , Fototerapia/métodos , Animales , Coagulación Sanguínea , Línea Celular Tumoral , Supervivencia Celular , Femenino , Hemólisis , Humanos , Inmunohistoquímica , Neoplasias Mamarias Animales/patología , Ensayo de Materiales , Ratones Endogámicos BALB C , Ratones Desnudos , Molibdeno , Inducción de Remisión
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