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Métodos Terapéuticos y Terapias MTCI
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1.
IEEE J Biomed Health Inform ; 28(4): 2223-2234, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38285570

RESUMEN

Preterm birth is the leading cause of death in children under five years old, and is associated with a wide sequence of complications in both short and long term. In view of rapid neurodevelopment during the neonatal period, preterm neonates may exhibit considerable functional alterations compared to term ones. However, the identified functional alterations in previous studies merely achieve moderate classification performance, while more accurate functional characteristics with satisfying discrimination ability for better diagnosis and therapeutic treatment is underexplored. To address this problem, we propose a novel brain structural connectivity (SC) guided Vision Transformer (SCG-ViT) to identify functional connectivity (FC) differences among three neonatal groups: preterm, preterm with early postnatal experience, and term. Particularly, inspired by the neuroscience-derived information, a novel patch token of SC/FC matrix is defined, and the SC matrix is then adopted as an effective mask into the ViT model to screen out input FC patch embeddings with weaker SC, and to focus on stronger ones for better classification and identification of FC differences among the three groups. The experimental results on multi-modal MRI data of 437 neonatal brains from publicly released Developing Human Connectome Project (dHCP) demonstrate that SCG-ViT achieves superior classification ability compared to baseline models, and successfully identifies holistically different FC patterns among the three groups. Moreover, these different FCs are significantly correlated with the differential gene expressions of the three groups. In summary, SCG-ViT provides a powerfully brain-guided pipeline of adopting large-scale and data-intensive deep learning models for medical imaging-based diagnosis.


Asunto(s)
Conectoma , Nacimiento Prematuro , Femenino , Niño , Humanos , Recién Nacido , Preescolar , Encéfalo/diagnóstico por imagen , Imagen por Resonancia Magnética/métodos , Conectoma/métodos , Suministros de Energía Eléctrica
2.
Adv Sci (Weinh) ; 9(17): e2200005, 2022 06.
Artículo en Inglés | MEDLINE | ID: mdl-35484709

RESUMEN

Sonodynamic therapy (SDT) typically suffers from compromised anticancer efficacy owing to the low reactive oxygen species (ROS) yield and complicated tumor microenvironment (TME) which can consume ROS and support the occurrence and development of tumors. Herein, ultrathin-FeOOH-coated MnO2 nanospheres (denoted as MO@FHO) as sonosensitizers which can not only facilitate ultrasound (US)-triggered ROS but also tune the TME by hypoxia alleviation, H2 O2 consumption as well as glutathione (GSH) depletion are designed. The FeOOH coating will boost the production yield of singlet oxygen (1 O2 ) and hydroxyl radicals (• OH) by inhibiting the recombination of US-initiated electron-hole pairs and Fenton-like reaction, respectively. Additionally, the catalase-like and GSH peroxidase-like activities of MO@FHO nanospheres enable them to break the TME equilibrium via hypoxia alleviation and GSH depletion. The combination of high ROS yield and fundamental destruction of TME equilibrium results in satisfactory antitumor outcomes, as demonstrated by the high tumor suppression efficacy of MO@FHO on MDA-MB-231-tumor-bearing mice. No obvious toxicity is detected to normal tissues at therapeutic doses in vivo. The capability to modulate the ROS production and TME simultaneously can afford new probability for the development of advanced sonosensitizers for synergistic comprehensive cancer therapy.


Asunto(s)
Neoplasias , Microambiente Tumoral , Animales , Glutatión/uso terapéutico , Hipoxia , Compuestos de Manganeso/farmacología , Compuestos de Manganeso/uso terapéutico , Ratones , Neoplasias/terapia , Óxidos/farmacología , Óxidos/uso terapéutico , Especies Reactivas de Oxígeno
3.
Pak J Pharm Sci ; 28(3 Suppl): 1159-62, 2015 May.
Artículo en Inglés | MEDLINE | ID: mdl-26051739

RESUMEN

To investigate pathogen distribution and drug resistance of incision infection caused by vascular operation to reduce postoperative incision infection, this paper retrospectively reviewed and analyzed 635 in-hospital patients taking vascular operation during Jan. 2008 and Dec. 2012. Analyzed data were statistically processed by SPSS 13.0 software, which resulted in 16 infected cases with 2.52% infection rate. A total of 27 pathogens wasisolated from specimens submitted for inspection, including 17 strains of Gram positive bacteria (62.96%) and 10 Gram negative bacteria (37.04%). Besides high sensitivity to imipenem, all bacteria were able to resist antibacterial drugs. Incision infection is proved in this research to be reduced effectively by some means, like complication correction before operation and reasonable application of antibacterial drugs after operation. While during an operation, it is necessary to operate strictly in a bacterium-free environment and wash incisions thoroughly.


Asunto(s)
Antibacterianos/uso terapéutico , Bacterias/efectos de los fármacos , Farmacorresistencia Bacteriana Múltiple , Infección de la Herida Quirúrgica/tratamiento farmacológico , Infección de la Herida Quirúrgica/microbiología , Procedimientos Quirúrgicos Vasculares/efectos adversos , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Bacterias/aislamiento & purificación , Femenino , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Estudios Retrospectivos , Infección de la Herida Quirúrgica/diagnóstico , Factores de Tiempo , Adulto Joven
4.
J Phys Chem B ; 117(16): 4713-22, 2013 Apr 25.
Artículo en Inglés | MEDLINE | ID: mdl-23406418

RESUMEN

The E. coli Lac repressor (LacI) tetramer binds simultaneously to a promoter-proximal DNA binding site (operator) and an auxiliary operator, resulting in a DNA loop, which increases repression efficiency. Induction of the lac operon by allolactose reduces the affinity of LacI for DNA, but induction does not completely prevent looping in vivo. Our previous work on the conformations of LacI loops used a hyperstable model DNA construct, 9C14, that contains a sequence directed bend flanked by operators. Single-molecule fluorescence resonance energy transfer (SM-FRET) on a dual fluorophore-labeled LacI-9C14 loop showed that it adopts a single, stable, high-FRET V-shaped LacI conformation. Ligand-induced changes in loop geometry can affect loop stability, and the current work assesses loop population distributions for LacI-9C14 complexes containing the synthetic inducer IPTG. SM-FRET confirms that the high-FRET LacI-9C14 loop is only partially destabilized by saturating IPTG. LacI titration experiments and FRET fluctuation analysis suggest that the addition of IPTG induces loop conformational dynamics and re-equilibration between loop population distributions that include a mixture of looped states that do not exhibit high-efficiency FRET. The results show that repression by looping even at saturating IPTG should be considered in models for regulation of the operon. We propose that persistent DNA loops near the operator function biologically to accelerate rerepression upon exhaustion of inducer.


Asunto(s)
Proteínas de Escherichia coli/metabolismo , Isopropil Tiogalactósido/metabolismo , Represoras Lac/metabolismo , Sitios de Unión , ADN/metabolismo , Ensayo de Cambio de Movilidad Electroforética , Escherichia coli/metabolismo , Proteínas de Escherichia coli/química , Transferencia Resonante de Energía de Fluorescencia , Colorantes Fluorescentes/química , Isopropil Tiogalactósido/química , Represoras Lac/química , Fósforo/química
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