Asunto(s)
Genes MHC Clase I/genética , Gorilla gorilla/genética , Antígenos de Histocompatibilidad Clase I/genética , Pan troglodytes/genética , Alelos , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Animales , Linfocitos B/metabolismo , Secuencia de Bases , Línea Celular , Cisteína/genética , ADN Complementario/genética , Disulfuros , Exones/genética , Gorilla gorilla/inmunología , Antígenos de Histocompatibilidad Clase I/química , Datos de Secuencia Molecular , Pan troglodytes/inmunología , Filogenia , Reacción en Cadena de la Polimerasa , Seudogenes/genética , Homología de Secuencia de AminoácidoRESUMEN
The use of the simian immunodeficiency virus (SIV) macaque model for assessing human immunodeficiency virus vaccine strategies will be facilitated by the characterization of predominant SIV cytotoxic T-lymphocyte (CTL) epitopes and their restricting major histocompatibility complex (MHC) class I molecules in macaque species. We now define a rhesus monkey SIVmac CTL epitope in the third hypervariable region of the envelope glycoprotein of the virus. This epitope, YNLTMKCR, contains the first two amino acids of a cysteine-cysteine loop which is the SIVmac analog of the human immunodeficiency virus type 1 V3 loop. We also employed one-dimensional isoelectric focusing to characterize the MHC class I molecule of the rhesus monkey that binds this SIVmac envelope peptide fragment. Cloning and sequencing the cDNA encoding this rhesus monkey MHC class I molecule demonstrates that it is a newly described HLA-A homolog, Mamu-A*02. This viral CTL epitope and its restricting MHC class I molecule will facilitate the use of the SIVmac rhesus monkey model for studies of envelope-based vaccine strategies and for exploring AIDS immunopathogenesis.