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Métodos Terapéuticos y Terapias MTCI
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1.
J Hazard Mater ; 239-240: 135-41, 2012 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-23022415

RESUMEN

This study investigated the change of hazardous materials in coking wastewater at different treatment stages (anaerobic, anaerobic/aerobic, anaerobic/aerobic/photo degradation, anaerobic/aerobic/ozone oxidation treatment) and the effects of them on the development of maize embryos and the activity of amylase and protease in maize seeds. Moreover the interaction of refractory organic matters in the wastewater at different treatment stages with amylase and protease also were determined in vitro. The results show that the biodegradable and the refractory organic compounds in the wastewater both can affect maize embryo development (germination inhibition rate is 19.3% for biodegradable organic compounds). As the treatment stage preceding, the inhibition effect of coking wastewater on the development of the maize embryo (for germination inhibition rates change from 49.3% to 24.6%) and on enzymatic activity (inhibition rates change from 63.9% to 22.4% for amylase) decreases gradually, but the photo-degradation treatment to anaerobic/aerobic effluent can increase its toxicity. The changes in the ability of the refractory organic compounds to bind with enzyme proteins, combined with the analysis of the organic components by GC/MS, show that in the process of coking wastewater treatment no new toxic chemicals were produced.


Asunto(s)
Coque , Semillas/efectos de los fármacos , Aguas Residuales/toxicidad , Contaminantes Químicos del Agua/toxicidad , Zea mays/efectos de los fármacos , Amoníaco/análisis , Amoníaco/toxicidad , Amilasas/metabolismo , Análisis de la Demanda Biológica de Oxígeno , Cianuros/análisis , Cianuros/toxicidad , Germinación/efectos de los fármacos , Péptido Hidrolasas/metabolismo , Semillas/enzimología , Semillas/crecimiento & desarrollo , Eliminación de Residuos Líquidos/métodos , Aguas Residuales/análisis , Contaminantes Químicos del Agua/análisis , Zea mays/enzimología , Zea mays/crecimiento & desarrollo
2.
J Photochem Photobiol B ; 98(2): 152-8, 2010 Feb 12.
Artículo en Inglés | MEDLINE | ID: mdl-20044266

RESUMEN

The effects of supplemental UV-B radiation on Taxus chinensis var. mairei were studied. Leaf traits, gas exchange parameters and the concentrations of photosynthetic pigments, cellular defense system products, secondary metabolites and ultrastructure were determined. UV-B radiation significantly decreased leaf area (p<0.05). Leaf number, secondary branch number, leaf weight per plant and leaf moisture all increased dramatically (p<0.05). Neither the leaf weight nor the specific leaf weight (SLW) exhibited significant differences between ambient and enhanced UV-B radiation. Gas exchange parameters were all dramatically reduced by enhanced UV-B radiation (p<0.05). The contents of chlorophyll and the chlorophyll a/b ratio were not distinctly affected by UV-B radiation, while carotenoids content significantly decreased (p<0.05). Supplemental UV-B treatment induced significant flavonoid accumulation (p<0.05), which was able to protect plant from radiation damage. Meanwhile, the appendage content, abaxial stomatal density, papilla density and particulate matter content in substomatic chambers increased noticeably by supplemental UV-B radiation, whereas the aperture size of single stomata was diminished. The number and area of plastoglobuli were apparently reduced by UV-B radiation, but stroma and grana lamellae were not destroyed. Our results demonstrated that T. chinensis var. mairei can activate several defense mechanisms against oxidative stress injury caused by supplemental UV-B radiation.


Asunto(s)
Taxus/efectos de la radiación , Rayos Ultravioleta , Ascorbato Peroxidasas , Catalasa/metabolismo , Flavonoides/análisis , Paclitaxel/análisis , Peroxidasas/metabolismo , Hojas de la Planta/química , Hojas de la Planta/efectos de la radiación , Hojas de la Planta/ultraestructura , Transpiración de Plantas , Superóxido Dismutasa/metabolismo , Taxus/química , Taxus/enzimología
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