RESUMEN
Two actinobacterial strains, CPCC 203464(T) and CPCC 203448, isolated from surface-sterilized stems of medicinal plants were subjected to a polyphasic taxonomic study. These two aerobic organisms formed pale yellow colonies on tryptic soy agar (TSA). Cells were Gram-stain-positive, non-acid-fast, non-motile, rod- or coccoid-like elements. Comparative 16S rRNA gene sequence analysis indicated that strains CPCC 203464(T) and CPCC 203448 were most closely related to the type strains of the species of the genus Williamsia. Chemotaxonomic properties such as containing meso-diaminopimelic acid in the cell wall, arabinose, galactose and ribose being the whole-cell hydrolysate sugars, phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylinositol (PI) as the phospholipids, and C16â:â0, 10-methyl C18â:â0, C18â:â1ω9c, C16â:â1ω7c and/or iso-C15â:â0 2-OH as major fatty acids supported the affiliation of strains CPCC 203464(T) and CPCC 203448 to the genus Williamsia. The DNA-DNA hybridization values in combination with differentiating chemotaxonomic and physiological characteristics strongly suggested that these two isolates should be classified as representatives of a novel species of the genus Williamsia. The name Williamsia sterculiae sp. nov. is proposed, with strain CPCC 203464(T) (â=âDSM 45741(T)â=âKCTC 29118(T)) as the type strain.
Asunto(s)
Actinomycetales/clasificación , Filogenia , Plantas Medicinales/microbiología , Sterculia/microbiología , Actinomycetales/genética , Actinomycetales/aislamiento & purificación , Técnicas de Tipificación Bacteriana , China , ADN Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Grasos/química , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Fosfolípidos/química , Tallos de la Planta/microbiología , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
A Gram-stain-positive, rod-shaped actinobacterium, designated strain I10A-01402(T), was isolated from surface-sterilized roots of a medicinal plant, Perilla frutescens, collected in a suburb of Beijing, China. Chemotaxonomically, the strain contained ll-diaminopimelic acid as the diagnostic diamino acid and MK-8(H4) as the predominant menaquinone. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The major fatty acids were C17â:â1ω9c, C18â:â1ω9c, C17â:â0, C16â:â0 and iso-C16â:â0. The genomic DNA G+C content was 70.4 mol%. 16S rRNA gene sequence analysis indicated that strain I10A-01402(T) belonged to the genus Nocardioides. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate formed a robust cluster with Nocardioides ginsengisegetis Gsoil 485(T), N. koreensis MSL-09(T) and N. alkalitolerans KSL-1(T). On the basis of the evidence from our polyphasic taxonomic study, a novel species, Nocardioides perillae sp. nov., is proposed. The type strain is I10A-01402(T) (â=âCPCC 203382(T) â=âDSM 24552(T) â=âKCTC 29022(T)).
Asunto(s)
Actinomycetales/clasificación , Perilla frutescens/microbiología , Filogenia , Raíces de Plantas/microbiología , Actinomycetales/genética , Actinomycetales/aislamiento & purificación , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácido Diaminopimélico/análisis , Ácidos Grasos/análisis , Datos de Secuencia Molecular , Fosfolípidos/análisis , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Microbiología del Suelo , Vitamina K 2/análogos & derivados , Vitamina K 2/análisisRESUMEN
An endophytic actinomycete, designated strain I10A-01259(T), was isolated from a surface-sterilized fruit of Lonicera maackii (Rupr.) Maxim., a medicinal plant, which was collected from a suburb of Beijing, China. Whole-cell hydrolysates of the isolate contained galactose and meso-diaminopimelic acid. The predominant phospholipids were phosphatidylglycerol and diphosphatidylglycerol; the menaquinones consisted mainly of MK-9, MK-11 and MK-12, with a minor amount of MK-10. The major fatty acids were iso-C16â:â0, anteiso-C15â:â0 and iso-C15â:â0. Comparative analysis of 16S rRNA gene sequences indicated that strain I10A-01259(T) was most closely related to Nocardiopsis arabia S186(T) (93.2â% sequence similarity), Thermobifida halotolerans YIM 90462(T) (93.0â%) and other strains of genera within the families Nocardiopsaceae and Thermomonosporaceae. On the phylogenetic tree based on 16S rRNA gene sequences, strain I10A-01259(T) fell within the radius of the suborder Streptosporangineae, in which the strain formed a distinct lineage next to the genera of the families Nocardiopsaceae and Thermomonosporaceae. Based on the data from our polyphasic taxonomic study, a novel genus and species, Allonocardiopsis opalescens gen. nov., sp. nov., are proposed within the suborder Streptosporangineae. The type strain of Allonocardiopsis opalescens is strain I10A-01259(T) (â=âCPCC 203428(T) â=âDSM 45601(T) â=âKCTC 19844(T)).