RESUMEN
Obesity is a severe public health problem worldwide. Our study aims to assess whether a saponin-enriched extract from the leaves of Boussingaultia gracilis (SBG) could attenuate obesity and its related metabolic disorders in mice and to explore the potential mechanism of this effect. Three-week old male mice were fed with a HFD for 7 weeks followed by a 10-week period in which the mice were supplemented with distilled water or SBG (250 mg per kg bodyweight). We demonstrated that SBG supplementation for 10 weeks mitigated obesity and its complications in diet-induced obesity (DIO) mice, such as reducing the hepatic damage underlying steatosis, modulating lipid metabolism, enhancing adipocyte thermogenesis, restoring insulin sensitivity and glucose homeostasis, and alleviating inflammation status. Finally, we established an untargeted metabolomics approach based on UPLC-MS to profile the metabolic changes in liver tissue and thereby discovered novel potential biomarkers to clarify the mechanisms of action of SBG in treating a mouse model of DIO. Thirty-nine potential biomarkers and five metabolic pathways contributing to the beneficial effect of SBG were discovered and identified.
Asunto(s)
Caryophyllales/química , Obesidad/prevención & control , Saponinas/farmacología , Adipocitos/efectos de los fármacos , Adipocitos/metabolismo , Animales , Proteína C-Reactiva/metabolismo , Colesterol/sangre , Dieta Alta en Grasa , Modelos Animales de Enfermedad , Prueba de Tolerancia a la Glucosa , Inflamación/tratamiento farmacológico , Resistencia a la Insulina , Metabolismo de los Lípidos/efectos de los fármacos , Hígado/metabolismo , Masculino , Metabolómica , Ratones , Ratones Endogámicos ICR , Ratones Obesos , FN-kappa B/genética , FN-kappa B/metabolismo , PPAR alfa/genética , PPAR alfa/metabolismo , Extractos Vegetales/farmacología , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/genética , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/metabolismo , Triglicéridos/sangreRESUMEN
OBJECTIVE: To explore the protecting mechanism of Yixintong for heart ischemia-reperfusion injury at cellular and subcellular levels, by observing the effects of Yixintong on three kinds of calcium channels. METHOD: The effects of Yixintong on Ca2+ influx on leak calcium channel, receptor-operationg calcium channel (ROC) and pulse-dependent calcium channel (PDC) were observed respectively, by using rat aortic smooth muscle cell and radioactive 45Ca technique. RESULT: Yixintong has no effects on leak calcium channel, but can inhibit the Ca2+ influx in ROC and PDC significantly. CONCLUSION: Yixintong can inhibit the Ca2+ influx in slow channel in a dose-dependent manner.