RESUMEN
BACKGROUND: Chinese medicine (CM) has become a popular interventional treatment for rheumatoid arthritis (RA). However, limited knowledge about general characteristics and long-term clinical outcomes hampers the development of CM for RA. PURPOSE: The main objectives of the China Rheumatoid Arthritis Registry of Patients with Chinese Medicine (CERTAIN) were to describe the population of RA patients receiving CM treatment in multiple centers in China using different variables and compare these findings with internationally reported data. STUDY DESIGN: The CERTAIN is a prospective, multicenter, observational disease registry. METHODS: Adult RA patients who fulfilled the 2010 American College of Rheumatology/ European League Against Rheumatism classification criteria for RA and received CM treatment were recruited into the CERTAIN by rheumatologists from 145 hospitals across 30 provinces in China. Data on demographics, disease characteristics, comorbidities, treatments, and adverse events, with a 2-year follow-up, were collected and documented using a predefined protocol. RESULTS: In the 2 years since the study began in September 2019, 11,764 patients have been enrolled (enrolment is ongoing), and 13.10% of participants have completed the 6-month follow-up. We present the baseline characteristics of the first 11,764 enrollees. CONCLUSIONS: The CERTAIN is the first nationwide registry to document comprehensive data on CM treatment in patients with RA. The development of the CERTAIN resource is a significant step forward for Chinese RA patients, herbal medicine users, and research communities and will deepen our understanding of CM for RA. REGISTRATION: The study was registered at ClinicalTrials.gov (NCT05219214).
Asunto(s)
Antirreumáticos , Artritis Reumatoide , Adulto , Antirreumáticos/uso terapéutico , Artritis Reumatoide/tratamiento farmacológico , Artritis Reumatoide/epidemiología , China/epidemiología , Humanos , Medicina Tradicional China , Estudios Prospectivos , Sistema de RegistrosRESUMEN
PURPOSE: Leukemia causes annually a significant number of deaths. The main objective of this study was to investigate the anticancer properties of piperine and curcumin against HL60 leukemia cells and to elucidate the underlying mechanism. METHODS: Antiproliferative effects were assessed by WST-1 cell viability assay. Cell apoptotic effects were studied by DAPI staining assay. Annexin V/propidium iodide (PI) assay was used to assess apoptosis. Electron microscopy was used for detection of autophagy and flow cytometry for cell cycle analysis, while transwell migration assay was used to study the effects on cell migration and invasion. Protein expression was estimated by western blot. RESULTS: The results showed that both piperine and curcumin inhibited significantly the growth of the HL60 cells and exhibited an IC50 of 25 and 30 µM respectively. Further, it was observed that the anticancer effects of piperine and curcumin were due to the induction of mitochondrial-mediated apoptosis which was also associated with enhancement of the Bax expression. Transmission electron microscopy also showed that both curcumin and piperine induced autophagy in the HL-60 leukemia cells. Flow cytometry showed that piperine and curcumin also caused arrest of the HL-60 cells at the S-phase of the cell cycle. Finally wound healing and transwell assays showed that piperine and curcumin suppressed the migration and invasive potential of the HL60 cells. CONCLUSIONS: The present study reveals that piperine and curcumin exhibit significant antitumor activity in human leukemia HL60 cells via multiple mechanisms and may prove promising in the development of systemic therapy for leukemia.
Asunto(s)
Alcaloides/uso terapéutico , Antineoplásicos/uso terapéutico , Apoptosis/efectos de los fármacos , Autofagia/efectos de los fármacos , Benzodioxoles/uso terapéutico , Puntos de Control del Ciclo Celular/efectos de los fármacos , Curcumina/uso terapéutico , Leucemia/tratamiento farmacológico , Piperidinas/uso terapéutico , Alcamidas Poliinsaturadas/uso terapéutico , Movimiento Celular , Humanos , Leucemia/patología , Invasividad Neoplásica , Fase SRESUMEN
The present study aimed to investigate the antiarthritic effect of physcion 8Oßglucopyranoside (POGD) and its possible mechanisms. The antiproliferative effects of POGD on MH7A cells were detected using a CCK8 assay, and the release of proinflammatory cytokines, interleukin (IL)1ß, IL6, IL8, IL12 and IL17A, were determined by ELISA. A type II collageninduced arthritis (CIA) rat model was established to evaluate the antiarthritic effect of POGD in vivo. The paw volumes, arthritis indices and serum levels of tumor necrosis factor (TNF)α, IL1ß, IL6, IL8, IL17A were determined by ELISA. The mRNA expression levels of matrix metalloproteinase (MMP)2, MMP3, MMP9, vascular endothelial growth factor and cyclooxygenase2 were determined by reverse transcriptionquantitative polymerase chain reaction analysis, and the expression levels of transforming growth factor (TGF)ß1, small mothers against decapentaplegic (Smad)4, Smad7, cJun Nterminal kinase (JNK), phosphorylated (p)JNK, pP38, P38, pextracellular signalregulated kinase (ERK)1/2, ERK1/2, nuclear factor (NF)κB p65 in the nucleus (N), cytosolic NFκB p65 (C), and inhibitor of NFκB (IκB) were determined by western blot analysis. The results indicated that POGD significantly inhibited MH7A cell growth. POGD markedly inhibited paw swelling and the arthritis indices of the CIA rats, and POGD may also inhibit the release of proinflammatory cytokines. Furthermore, POGD downregulated the expression levels of TGFß1, Smad4, NFκB p65 (N), p38, pp38, pERK1/2, JNK, pJNK, TGFß1, Smad4, pJNK, JNK, pP38, P38, pERK1/2, ERK1/2 and NFκB p65 (N), and upregulated the Smad7, NFκB p65 (C) and IκB in TNFα induced MH7A cells. In conclusion, the results suggested that POGD is a promising potential antiinflammatory drug, and that POGD may decrease the expression of proinflammatory cytokines and mediators via inhibiting the TGFß/NFκB/mitogenactivated protein kinase pathways.