Effect of Danggui-Shaoyao-San-Containing Serum on the Renal Tubular Epithelial-Mesenchymal Transition of Diabetic Nephropathy.

OBJECTIVES: To investigate the effect of Danggui-Shaoyao-San (DSS)-containing serum on the renal tubular Epithelial-Mesenchymal Transition (EMT) of Diabetic Nephropathy (DN) in high glucose- induced HK-2 cells and its mechanism. METHODS: 20 rats were randomly divided into four groups: blank control group, DSS low dose group (DSS-L), DSS middle dose group (DSS-M), and DSS high dose group (DSS-H). DSS was administrated to the corresponding group (7g/kg/d, 14g/kg/d and 21g/kg/d) for 7 consecutive days, and the same volume of saline was given to the blank control group by gavage. The rat drug-containing serum was successfully prepared. HK-2 cells were divided into five groups: blank control group, model group, DSS-L, DSS-M, DSS-H, according to the corresponding drug and dose of each treatment group. Protein and mRNA levels of Jagged1, Notch1, Hes5, Notch Intracellular Domain (NICD), E-cadherin, alpha- Smooth Muscle Actin (α-SMA) and vimentin at 24h, 48h and 72h were detected by Western Blot and RT-qPCR. RESULTS: The protein and mRNA levels of Jagged1, Notch1, Hes5, NICD, α-SMA and vimentin in the treatment groups were remarkably decreased compared with the model group (P<0.05), and the protein and mRNA levels of E-cadherin were notably increased (P<0.05) by Western Blot and RT-qPCR. CONCLUSION: Our results demonstrated that DSS could prevent DN by ameliorating renal tubular EMT through inhibition of the Notch signaling pathway.

Clinical effect of Shiquan Dabu Decoction combined with nitroglycerin on acute heart failure after myocardial infarction / 中国基层医药

Objective To summarize and discuss the clinical effect of Shiquan Dabu Decoction combined with nitroglycerin in the treatment of heart failure after acute myocardial infarction .Methods From January 2016 to June 2017,110 patients with heart failure after acute myocardial infarction admitted to the Department of Cardiology of Lishui Hospital of Traditional Chinese Medicine were selected as study objects .The patients were randomly divided into observation group and control group according to the digital table ,with 55 cases in each group.The control group was treated with routine nitroglycerin therapy ,while the observation group was treated with routine nitroglycerin therapy combined with Shiquan Dabu Decoction.The therapeutic effect of the two groups after one week of treatment was observed.Results The total effective rate in the observation group was 92.73%,which was significantly higher than that of the control group (78.18%),and the difference between the two groups was statistically significant ( χ2 ＝4.681,P＜0.05).The HR level of the observation group [(82.5 ±6.5)times／min] was significantly lower than that of the control group[(102.6 ±3.9)times／min](t＝19.655,P＜0.01).The levels of SBP[(116.5 ±6.4)mmHg] and SpO2[(95.2 ±1.6)%] in the observation group were significantly higher than those in the control group [(108.8 ± 5.2)mmHg,(92.5 ±1.2)%],there were statistically significant differences between the two groups ( t ＝6.925, 10.012,all P＜0.01).The incidence of adverse reaction in the observation group was 10.91%,which was higher than that in the control group (3.64%),but there was no statistically significant difference (χ2 ＝2.157,P＞0.05). Conclusion For patients with heart failure after acute myocardial infarction ,the combination of traditional Chinese medicine Shiquan Dabu Decoction in clinical routine treatment has better effect and is worthy of popularizing .

Clinical effect of Shiquan Dabu Decoction combined with nitroglycerin on acute heart failure after myocardial infarction / 中国基层医药

Objective@#To summarize and discuss the clinical effect of Shiquan Dabu Decoction combined with nitroglycerin in the treatment of heart failure after acute myocardial infarction.@*Methods@#From January 2016 to June 2017, 110 patients with heart failure after acute myocardial infarction admitted to the Department of Cardiology of Lishui Hospital of Traditional Chinese Medicine were selected as study objects.The patients were randomly divided into observation group and control group according to the digital table, with 55 cases in each group.The control group was treated with routine nitroglycerin therapy, while the observation group was treated with routine nitroglycerin therapy combined with Shiquan Dabu Decoction.The therapeutic effect of the two groups after one week of treatment was observed.@*Results@#The total effective rate in the observation group was 92.73%, which was significantly higher than that of the control group(78.18%), and the difference between the two groups was statistically significant(χ2=4.681, P<0.05). The HR level of the observation group[(82.5±6.5)times/min] was significantly lower than that of the control group[(102.6±3.9)times/min](t=19.655, P<0.01). The levels of SBP[(116.5±6.4)mmHg]and SpO2[(95.2±1.6)%] in the observation group were significantly higher than those in the control group[(108.8±5.2)mmHg, (92.5±1.2)%], there were statistically significant differences between the two groups(t=6.925, 10.012, all P<0.01). The incidence of adverse reaction in the observation group was 10.91%, which was higher than that in the control group(3.64%), but there was no statistically significant difference (χ2=2.157, P>0.05).@*Conclusion@#For patients with heart failure after acute myocardial infarction, the combination of traditional Chinese medicine Shiquan Dabu Decoction in clinical routine treatment has better effect and is worthy of popularizing.

Clinical observation on treatment of diabetic peripheral neuropathy bytraditional Chinese medicine foot bath combined with Xueshuantong / 中国生化药物杂志

Objective To observe the clinical efficacy of traditional Chinese medicine foot bath combined with Xueshuantong injection in the treatment of diabetic peripheral neuropathy.Methods Sixty patients with diabetic peripheral neuropathy(DPN)were randomly divided into two groups.According to the patients' condition,oral hypoglycemic drugs or insulin were used to control blood glucose.The control group was treated with Xueshuantong injection on routine nutrition nerve program.In the conventional nutrition nerve program based on the use of traditional Chinese medicine foot bath combined with Xueshuantong injection treatment,2 groups were treated after 10 days to evaluate the efficacy.Results The total effective rate of the observation group and the control group was 90％and 66.7％respectively.The sensory and motor function of the observation group were significantly improved after the treatment group,and the blood rheology and serum endothelin level were significantly decreased.Conclusion Chinese medicine foot bath combined with Xueshuantong injection for the treatment of diabetic peripheral neuropathy.

Application of point massage combined with physiotherapy in the treatment of insomnia for the community elderly / 中国基层医药

Objective To observe the application effect of point massage combined with physiotherapy in the treatment of insomnia for community elderly.Methods 30 elderly patients with insomnia who accepted point massage combined with physiotherapy were selected as observation group,and 30 elderly patients treated in tertiary hospital with insomnia who took alprazolamum for treatment were selected as control group.The SRSS score of the two groups before and after treatment,as well as treatment efficacy were compared.Results The insomnia treatment efficacy of the observation group was higher than that of the control group(Z =2.45,P <0.05).The SRSS rating of the observation group before treatment was (30.76 ±7.1 9)points and after treatment was (1 5.43 ±3.95)points.The SRSS rating of the control group before treatment was (32.33 ±6.98)points,which after treatment was (21 .93 ±6.73)points.The SRSS scores of both two groups after treatment were decreased (t =1 0.23,5.87,all P <0.05),with obvious differ-ences.The SRSS scores of the observation group decreased more obviously and was lower than that of the control group (t =4.56,P <0.05).Conclusion The point massage combined with physiotherapy can effectively improve insomnia of the elderly,which is also with advantages of easy operation and no obvious side effects and high cooperation degree etc,and it is suitable to be promoted in community.

Effect of Electroacupuncture with Acupoint Selection by Heavenly Stems on Eosinophil Apoptosis in Guinea Pigs with Asthma / 广州中医药大学学报

Objective To investigate the therapeutic effect of acupoint selection by heavenly stems on eosinophils(EOS) in guinea pigs with asthma.Methods Forty-eight guinea pigs were randomized into four groups: group A(timely electroacupuncture at the period of the day from 3 p.m.to 5 p.m.),group B(un-timely electroacupuncture at the period of the day from 9 a.m.to 11 a.m.),group C(model group) and group D(normal control group).Asthma was induced by chicken ovalbumin in groups A,B and C.The hematoxylin-eosin(HE) staining method and terminal-deoxynucleotidyl transferase-mediated nick end labeling(TUNEL) method were applied in the pathological and quantitative observation of eosinophils(EOS) in lung slices.Results The number of EOS was increased in model group(P