RESUMEN
Chemical investigations of the aerial parts of Tibetan folk medicine Delphinium chrysotrichum resulted in the isolation of two new diterpenoid alkaloids, delphatisine D (1) and chrysotrichumine A (2), together with ten known diterpenoid alkaloids (3-12) and 3ß,6α-dihydroxysclareolida (13). Delphatisine D (1) is a rare atisine-type alkaloid from genus Delphinium and is the C-15 epimer of spiramine C which bears an internal carbinolamine ether linkage (N-C-O-C) between C-7 and C-20. Chrysotrichumine A (2) is a rare natural C19-diterpenoid alkaloid possessing a nitrone group between C-17 and C-19. Their structures were elucidated on the basis of extensive spectroscopic analysis. Compound 13 is a sesquiterpene first isolated from this genus. Compounds 1, 3-7 and 13 were investigated for cytotoxicity against human breast cancer cell lines of MCF-7 and MDA-MB-23, none of them presented significant activity except compound 7 which exhibited slight activity at 100⯵M against MDA-MB-231, but did not reach the EC50.