RESUMEN
The aim of the study was the evaluation of the action of a new natural drug--Naran S, elaborated in the Department of Inorganic Chemistry of the Medical University of Lublin (patent registration P.332066) in the treatment of inflammatory conditions of the oral cavity mucous membrane. The experiment was conducted on male Wistar rats. Topical inflammation of the gum was induced by injecting a complete Freund adjuvant (AF) (Calbiochem) into the inter-dental papilla of the lower incisors. The material was taken, fixed and stained with hematoxylin and eosin and by the use of the Masson's method after 24 hours, 1, 2, and 4 weeks following the injection time. Morphology of the mucous membrane was examined and it was noticed that in the group treated with Naran S infusion the complete rebuilding of the epithelial strata, collagen fibres took place. The shape of the cells was regular and there occurred the elimination of the tissue fluid in the proper mucous membrane. The results obtained in the study allow to claim that the topically given plant drug thanks to its contents of biologically active substance such as: iridoids, flavonoids, phenol acids, tannins, displays curative activity in the therapy of the inflammatory conditions of the oral cavity mucous membrane.
Asunto(s)
Antiinflamatorios/farmacología , Flavonoides/farmacología , Gingivitis/tratamiento farmacológico , Iridoides/farmacología , Antisépticos Bucales/farmacología , Periodontitis/tratamiento farmacológico , Fenoles/farmacología , Fitoterapia , Extractos Vegetales/farmacología , Taninos/farmacología , Animales , Modelos Animales de Enfermedad , Adyuvante de Freund , Encía/efectos de los fármacos , Encía/patología , Gingivitis/patología , Masculino , Mucosa Bucal/efectos de los fármacos , Mucosa Bucal/patología , Periodontitis/patología , Periodoncio/efectos de los fármacos , Periodoncio/patología , Ratas , Ratas Wistar , Resultado del TratamientoRESUMEN
The effectiveness of the natural drug--Naran S was studied in the therapy of the topical inflammation of the oral cavity mucous membrane. The experiment was conducted on male Wistar rats that were subjected to induction of inflammatory condition by injecting Freund adjuvant and rinsing oral cavity with the infusion of the drug for the period of 28 days. The analysis of the ultra structure of the epithelium cells was conducted with the use of transmission electron microscope. It was observed that under the influence of the drug, the epithelium undergoes renewal, the cells are joined with thick desmosomes, the nuclei have the proper areolas and the mitochondrium matrix is not oedematic.