Asunto(s)
Glucosa/farmacología , Sustancias Macromoleculares/biosíntesis , Cloruro de Sodio/farmacología , Staphylococcus/metabolismo , Aminoácidos/análisis , Proteínas Bacterianas/biosíntesis , Bacteriólisis , Carbohidratos/análisis , Membrana Celular , Pared Celular/análisis , Medios de Cultivo , Citoplasma , ADN Bacteriano/biosíntesis , Hexosaminas/análisis , Cinética , Microscopía Electrónica , Mucoproteínas/análisis , Fósforo/análisis , Protoplastos , ARN Bacteriano/biosíntesis , Staphylococcus/análisis , Staphylococcus/citología , Staphylococcus/efectos de los fármacos , Staphylococcus/crecimiento & desarrolloRESUMEN
Lipopolysaccharide was prepared from the extracellular lipoglycopeptide produced by the lysine-requiring mutant Escherichia coli A.T.C.C. 12408 grown under lysine-limiting conditions. The lipid moiety, containing glucosamine phosphate and four fatty acids (lauric acid, myristic acid, beta-hydroxymyristic acid and palmitic acid) corresponded in composition to lipid A of known bacterial lipopolysaccharides. The components of the polysaccharide moiety were d-glucose, d-galactose, l-glycero-d-manno-heptose, 3-deoxy-2-oxo-octonic acid, ethanolamine and phosphate. These are the constituents of the polysaccharide of the cell-wall antigens from rough strains of E. coli. Lipopolysaccharides were also prepared from whole cells of E. coli 12408 grown with excess or limited amounts of lysine; they were identical in carbohydrate composition with the extracellular lipopolysaccharide. The biological properties of this material also resembled those of known lipopolysaccharides; it was antigenic, pyrogenic, toxic and had adjuvant activity.