Evaluation of the antioxidant characteristics of craft beer with green tea.

The addition of green tea as antioxidants to beer can improve the beer's flavor stability by protecting against staling during storage. To analyze the effect of different green teas on the flavor stability of beer, we developed an approach to rapidly evaluate their antioxidant activity. Ten types of craft beer were produced by adding different kinds of green tea during brewing, and their antioxidant activity and phenolic profiles were evaluated. The results showed remarkable variations in antioxidant activity and antioxidative compound contents, which were considerably higher in green tea beers than in non-tea beer (p < 0.05). A comprehensive evaluation function was developed to evaluate the total antioxidant activity of beers using principal component analysis. The highest total antioxidant activity was observed in Taiping Houkui beer, with a comprehensive evaluation score of 2.53. Pearson correlation analysis suggested that (-)-epigallocatechin gallate, (-)-epicatechin gallate, and (-)-epigallocatechin were strongly correlated with the total antioxidant activity of green tea beers (p < 0.01). The summation of their contents represented more than 60% of the total phenolic content of the teas, which can be used to predict the total antioxidant activity of green tea beers. PRACTICAL APPLICATION: Flavor stability is of prime concern for brewers, and flavor aging is increasingly becoming the limiting factor in beer shelf life. The application of green tea as antioxidants in beer can improve the flavor stability by protecting against beer staling during storage. The analytical method developed in this study will contribute to the rapid comparison of the effect of different green teas on the flavor stability of beer. Furthermore, the research findings demonstrate the potential benefits of green teas to beer flavor stability, which is of considerable importance in promoting the development and consumption of green teas.

Levan from Bacillus amyloliquefaciens JN4 acts as a prebiotic for enhancing the intestinal adhesion capacity of Lactobacillus reuteri JN101.

Improving intrinsic adhesion performance of the known probiotics facilitates their residence and colonization, and therefore exerts more beneficial effects on the human or animal host. In this study, through adaptive culture with levan, Lactobacillus reuteri JN101 achieved the same biomass and exhibited 2.6 times higher adhesion capacity to HT-29 cells than those grown with glucose. The mechanism study related to this adhesion enhancement showed that the elevated proportion of unsaturated fatty acids facilitated the bacterial cells to overcome repulsive forces to approach the intestinal epithelial cell. At the same time, and the greater amounts of cell membrane proteins, such as S-layer protein (3.2 folds), elongation factor Tu (2.6 folds) and phosphoglycerate kinase (2.4 folds) probably enhanced the complementary interactions to the receptor on the epithelial cell. These results presented here indicated levan could be used as a potential prebiotic to regulate the adhesion capacity of probiotics, and provide ground for developing the specific-probiotics oriented functional food.

Influence of nonionic and ionic surfactants on the antifungal and mycotoxin inhibitory efficacy of cinnamon oil nanoemulsions.

The influence of ionic surfactants (cationic surfactant lauric arginate and anionic surfactant lysolecithin) on the physical properties, antifungal and mycotoxin inhibitory efficacy of Tween 80 stabilized cinnamon oil-in-water nanoemulsions was investigated. Nanoemulsion droplets of similar particle diameter (∼100 nm), but variable electrical characteristics, were formed by mixing 0.1 wt% ionic surfactant with 0.9 wt% Tween 80 before homogenization. The nanoemulsions were physically stable over 28 days at 23 °C. The antifungal activity (against mycelial growth and spore germination) and mycotoxin inhibitory activity of cinnamon oil nanoemulsions bearing positive, neutral, and negative charge surface was then evaluated against two chemotypes of Fusarium graminearum. In general, the cinnamon oil played a decisive role in the resulting antifungal and mycotoxin inhibitory activities. The surfactant charge had a limited impact on the antifungal mycotoxin inhibitory activities of cinnamon oil in the nanoemulsions. Both ionic surfactant-based cinnamon oil nanoemulsions showed greater activity in inhibiting mycelial growth and mycotoxin production of F. graminearum than those based on Tween 80. Treatment of mycelium with cinnamon oil nanoemulsions resulted in the loss of cytoplasm from fungal hyphae, and accounted for the antifungal action. These results have important implications for the design of essential oil based nanoemulsions as effective antifungal delivery systems in foods.

Prevention and treatment effects of edible berries for three deadly diseases: Cardiovascular disease, cancer and diabetes.

Cardiovascular disease (CVD), cancer and diabetes are serious threat to human health and more and more aroused people's attention. It is important to find the safe and effective prevention and treatment methods for the three deadly diseases. At present, a generally attention in the possible positive effects of edible berries for the three deadly diseases has been noted. Berry phytochemical compounds regulate different signaling pathways about cell survival, growth and differentiation. They contribute to the prevention and treatment of CVD, cancer and diabetes. This article reviews previous experimental evidence, several common berry phytochemical compounds and their possible mechanisms involved in three deadly diseases were summarized.