RESUMEN
This study aimed to investigate the effects of 15-deoxyspergualin (DSG), tacrolimus (FK 506) and cyclosporin A (CyA), alone or in combination, on delayed xenograft rejection (DXR). We used the guinea-pig-to-C6-deficient (C6-)-PVG-rat heart transplantation model, since in this strain combination, hyperacute rejection is avoided. In C6- control rats, the guinea pig xenografts survived for 39.2 +/- 6.3 h (mean +/- SD). Splenectomy alone resulted in a xenograft survival of 71.8 +/- 7.8 h, but the addition of CyA or FK 506 did not further improve graft survival (73.6 +/- 3.0 h and 72.0 +/- 17.6 h, respectively). In contrast, DSG treatment increased graft survival to a mean of 99.8 +/- 9.2 h. When CyA or FK 506 was combined with DSG, no additional effects were observed (105 +/- 24.3 h and 95.1 +/- 5.6 h, respectively). DSG alone or in combination with FK 506 or CyA resulted in a significant reduction in the serum IgM levels and reduced the deposits of IgM and IgG in rejected grafts. However, all xenografts were still heavily infiltrated by ED1 + macrophages, regardless of the treatment used. Thus, DSG treatment resulted in moderate prolongation of xenograft survival in C6- rats. The effect seems to be related to suppression of xenoreactive antibody production. To prolong xenograft survival further, strategies that inhibit macrophage infiltration seem required.
Asunto(s)
Complemento C6/deficiencia , Rechazo de Injerto/prevención & control , Guanidinas/uso terapéutico , Cobayas/inmunología , Trasplante de Corazón/inmunología , Inmunosupresores/uso terapéutico , Ratas/inmunología , Trasplante Heterólogo/inmunología , Animales , Anticuerpos Heterófilos/biosíntesis , Anticuerpos Heterófilos/sangre , Terapia Combinada , Complemento C3/análisis , Complemento C6/inmunología , Ciclosporina/administración & dosificación , Ciclosporina/uso terapéutico , Evaluación Preclínica de Medicamentos , Sinergismo Farmacológico , Quimioterapia Combinada , Rechazo de Injerto/tratamiento farmacológico , Rechazo de Injerto/cirugía , Guanidinas/administración & dosificación , Inmunidad Celular , Inmunoglobulina G/análisis , Inmunoglobulina M/análisis , Inmunosupresores/administración & dosificación , Macrófagos/efectos de los fármacos , Miocardio/química , Miocardio/patología , Bazo/inmunología , Esplenectomía , Tacrolimus/administración & dosificación , Tacrolimus/uso terapéutico , Trasplante HeterotópicoRESUMEN
OBJECTIVE: To investigate the effect of treatment of cachexia with Umbellatus Polyporus Polysaccharide (UPP). METHODS: UPP was used to treat cachexia induced by toxohormone-L. RESULTS: Toxohormone-L from ascites fluid with primary hepatoma could induce lipolysis in vitro significantly, inhibit the intake behavior, decrease the level of blood zinc, increase that of blood copper (P < 0.01), upp could inhibit these effects. CONCLUSION: Toxohormone-L was closely related with neoplastic cachexia and UPP could inhibit this effect induced by toxohormone-L significantly.