Screening of cadmium resistant bacteria and their growth promotion of Sorghum bicolor (L.) Moench under cadmium stress.

Heavy metal pollution of agricultural soils, especially from cadmium (Cd) contaminationcaused serious problems in both food security and economy. Sorghum bicolor (L.) showed a great potential in phytoremediation of Cd contamination due to its fast growth, high yield and easy harvesting. However, the growth of S. bicolor plants tends to be inhibited under Cd exposure, which limited its application for Cd remediation. Plant growth-promoting rhizobacteria may enhance the Cd resistance of S. bicolor and thus improve its Cd removal efficiency. In this study, three Cd-resistant bacteria were screened based on Cd and acid tolerance and identified as Bacillus velezensis QZG6, Enterobacter cloacae QZS3 and Bacillus cereus QZS8, by 16S rRNA sequencing. Inoculation of hydroponic plants with strains QZG6, QZS3 or QZS8 significantly promoted the biomass of sorghum plants by 31.52%, 50.20% and 26.93%, respectively, compared with those of uninoculated plants under Cd exposure. The activity of SOD, POD and MDA content in Cd-stressed S. bicolor plants were reduced of 65.74%, 31.52%, and 80.91%, respectively, when inoculated with the strains QZS3. For pot experiment, strains QZG6, QZS3 and QZS8 significantly promoted the biomass of sorghum plants by 47.30%, 19.27% and 58.47%, compared with those of uninoculated plants under Cd exposure. The activity of SOD, POD and MDA content in Cd-stressed S. bicolor plants were reduced of 67.20%, 22.40%, and 40.65%, respectively, when inoculated with the strains QZS3. All these three strains significantly increased the Cd removal efficiency of the plants by 42.16% (QZG6), 18.76% (QZS3) and 21.06% (QZS8). To investigate the bacterial characteristics associated with growth promotion of S. bicolor plants, the ability on nitrogen fixation, phosphorus solubilization, siderophores production, and phytohormones production were determined. All the strains were able to fix nitrogen. Phosphorus release was observed for strains QZG6 (inorganic or organic phosphorus) and QZS3 (inorganic phosphorus). Both QZG6 and QZS8 were able to produce siderophores, while only QZG6 was positive for ACC deaminase. All the strains produced IAA, SA and GA. These results indicated that the three strains promoted the plant growth under Cd stress, probably through Cd detoxification by siderophores, as well as through growth regulation by N/P nutrient supply and phytohormone. The present study showed a great potential of the three Cd-resistant strains combined with S. bicolor plants in the remediation of Cd-polluted soils, which may provide a new insight into combining the advantages of microbes and plants to improve the remediation of Cd-contaminated soils.

Antioxidant stress and anticancer activity of peptide­chelated selenium in vitro.

The association between selenium and peptide in gastric cancer is an important research topic. The present study reported the facile synthesis of anticancer bioactive peptide (ACBP)­functionalized selenium (ACBP­S­Se) particles with enhanced anticancer activities and a detailed mechanistic evaluation of their ability to regulate oxidative stress in vitro. Structural and chemical characterizations were revealed by ultraviolet absorption, Fourier transform infrared, X­ray photoelectron, nuclear magnetic resonance carbon and hydrogen, energy dispersive X­ray spectroscopy and inductively coupled plasma mass spectrometry, as well as scanning electron microscopy. Sulfhydrylation modifications of ACBP were achieved with S­acetylmercaptosuccinic anhydride via chemical absorption. After the polypeptide was modified by sulfhydrylation, the ACBP chain was linked to sulfhydryl groups by amide bonds to form the ACBP­chelated selenium complex. Two gastric cancer cell lines (MKN­45 and MKN­74 cells) demonstrated high susceptibility to ACBP­S­Se particles and displayed significantly decreased proliferation ability following treatment. The results suggested that the bioactive peptide­chelated selenium particles effectively inhibited the proliferation of MKN­45 and MKN­74 cells in vitro. The genes encoding CDK inhibitor 1A (CDKN1A), cyclin B1, thioredoxin (TXN) and mitogen­activated protein kinase kinase kinase 5 are associated with regulation of oxidative stress, while CDKN1A and TXN protect cells by decreasing oxidative stress and promoting cell growth arrest. Therefore, ACBP­S­Se may be an ideal chemotherapeutic candidate for human cancer, especially gastric cancer.

Analysis of the Efficacy and Pharmacological Mechanisms of Action of Zhenren Yangzang Decoction on Ulcerative Colitis Using Meta-Analysis and Network Pharmacology.

OBJECTIVE: We analyzed the efficacy and pharmacological mechanisms of action of Zhen Ren Yang Zang decoction (ZRYZD) on ulcerative colitis (UC) using meta-analysis and network pharmacology. METHODS: The major databases were searched for randomized controlled trials of ZRYZD for the treatment of UC. Meta-analysis of the efficacy of ZRYZD on UC was conducted using RevMan software. Active compounds and target genes were acquired using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. UC-related genes were searched using the GeneCards database. Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using RGUI. A compound-target network was constructed using Cytoscape software, and a protein-protein interaction network was constructed using the STRING database. Molecular docking simulations of the macromolecular protein targets and their corresponding ligand compounds were performed using the AutoDock tool and AutoDock Vina software. RESULTS: Meta-analysis revealed that the total effective rate and recovery rate of clinical efficacy were significantly higher in the experimental group than those of the control group. The screening identified 169 active compounds and 277 active target genes for ZRYZD. The 277 active target genes were compared with the 4,798 UC-related genes. This identified 187 active target genes of ZRYZD for UC that correlated with 138 active compounds. GO functional enrichment and KEGG pathway enrichment analyses were performed, and compound-target and protein-protein interaction networks were constructed. The key compounds and key target proteins were then selected. Finally, target protein binding with the corresponding compound was analyzed using molecular docking. CONCLUSION: Our findings demonstrate the effectiveness and safety of ZRYZD for the treatment of UC and provide insight into the underlying pharmacological mechanisms of action. Furthermore, key compounds were identified, laying the foundation for future studies on ZRYZD for the treatment of UC.

The Synergistic Antitumor Effect of Tanshinone IIA Plus Adriamycin on Human Hepatocellular Carcinoma Xenograft in BALB/C Nude Mice and Their Influences on Cytochrome P450 CYP3A4 In Vivo.

OBJECTIVE: Hepatocellular carcinoma is one of the most common diseases that seriously threaten human life and health. In this study, we evaluated the inhibitory effect of tanshinone IIA (Tan IIA) combined with adriamycin (ADM) on human hepatocellular carcinoma and developed a platform to assess the function if Chinese herbal ingredients combined with chemotherapy drugs have synergistic antitumor effects in vivo. METHODS: Established animal model of human hepatocarcinoma HepG2 cell in nude mice. Mice were divided into model control group, Tan IIA group, ADM group, and Tan IIA + ADM group. The changes from general condition, weight, tumor volume, and inhibition rate were observed. The data were gathered from serum AST level and histopathological changes. The content and activity of cytochrome P450 were determined by spectrophotometric analysis. CYP3A4 protein expression was analyzed by western blotting. The binding model crystal structure of Tan IIA and ADM with pregnane X receptor (PXR) was evaluated by Discovery Studio 2.1. RESULTS: A combination of Tan IIA with ADM could improve life quality by relieving ADM toxicity, decreasing tumor volume, declining serum AST level, and improving liner pathological section in tumor-bearing mice. The inhibitory rates of Tan IIA, ADM, and cotreatment were 32.77%, 60.96%, and 73.18%, respectively. The Tan IIA group significantly enhanced the content of cytochrome b5, P450, and erythromycin-N-demethylase activity. CYP3A4 protein expression was enhanced obviously by the Tan IIA + ADM group. Virtual molecular docking showed that both Tan IIA and ADM could be stably docked with the same binding site of PXR but different interactions. CONCLUSIONS: Tan IIA in combination with ADM could improve the life quality in tumor-bearing mice and enhance the antitumor effect. The Tan IIA group increased the concentration of cytochrome P450 enzymes and activity. Combined Tan IIA with ADM could upregulate the CYP3A4 protein expression and make relevant interaction with protein PXR by virtual docking.

Yi-qi-yang-yin-tian-sui-fang enhances cisplatin-induced tumor eradication and inhibits interleukin-7 reduction in non-small cell lung cancer.

Traditional Chinese Medicine (TCM) has been recognized to be conducive to enhancing the efficiency and reducing the side effects in the whole course of cancer treatment. The mechanisms of TCM/chemotherapy combination involved with interleukin-7 (IL-7) potentially enhance immune responses against tumor. In the present study, we emphasized on a herbal formulation Yi-qi-yang-yin-tian-sui-fang or TCM for short, and investigated its roles in chemotherapy in non-small cell lung cancer (NSCLC). The mice bared with tumor were treated with cisplatin (DDP) and simultaneously administrated with/without low, medium and high doses of TCMs (effective content: 0.5, 2.0 and 8.0 g/per mice) via oral gavage. The results indicated that combination of TCM further elevated the therapy efficiency of DDP in a dose-dependent manner. The growth of tumor cells was estimated by Ki-67 stain and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) assay. The addition of TCM to the DDP treatment could significantly decrease the expression of Ki-67 and promote the apoptosis of tumor cells. In addition, the serum IL-7 level was down-regulated by DDP but restored by the treatment of TCM. The expression of IL-7 and its receptor IL-7R in tumor tissues was also recovered by TCM. Furthermore, the side effect from bone marrow suppression (myelosuppression) induced by DDP were assessed. TCM could abrogate DDP-induced apoptosis of bone marrow and also remarkably induced the expressions of IL-7 and hematopoietic growth factors including G-CSF, GM-CSF, SCF, and SDF-1 in bone marrow. These data indicated that this TCM combined with DDP showed superior anti-tumor effects with reduced myelosuppression via up-regulating IL-7.

Folic acid reverses uric acid crystal-induced surface OAT1 internalization by inhibiting RhoA activity in uric acid nephropathy.

To investigate how organic anion transporter (OAT)-1 is involved in uric acid nephropathy (UAN), a rat model for UAN was established and the serum uric acid, blood urea nitrogen and serum creatinine levels were all measured, and observed to be increased. It was additionally identified that in UAN rats the surface OAT1 expression levels were reduced. By treating HEK cells with monosodium urate (MSU) crystals, it was observed that the cells exhibited a reduction in OAT1 levels. Furthermore, MSU crystals were observed to recruit Ras homolog family member A (RhoA), a small guanosine triphosphatase, to the membrane and activate it. Following RhoA activation, the OAT1 internalization rate was identified to be increased. The dominant­negative RhoA N19 mutation was able to block MSU­induced OAT1 internalization, indicating that the process was RhoA­dependent. Finally, the results indicated that folic acid, a daily nutritional supplement, was capable of rescuing MSU­induced nephropathy and OAT1 internalization. These observations indicated that uric acid crystals were able to reduce the OAT1 membrane distribution through activating RhoA, and that folic acid was capable of preventing MSU-induced OAT1 relocation by inhibiting the RhoA signaling pathway.

Antidiarrheal properties of different extracts of Chinese herbal medicine formula Bao-Xie-Ning.

OBJECTIVE: Bao-Xie-Ning (BXN), a traditional Chinese herbal medicine (CHM) formula composed of Fructus Evodiae, Flos Caryophylli and Cortex Cinnamomi, and used for the treatment of infant diarrheal illness, was subject to systematic assessment for its putative multiple pharmacodynamic effects and pharmacological antidiarrheal mechanisms. METHODS: High-performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometric/mass spectrometry was developed and validated for identification and quantification of the main constituents in different extracts of BXN. Male Kunming mice weighing 20 to 25 g were used for detecting the antidiarrheal activity of the extracts. Ethanolic extract (EE), volatile oil extract (VOE), and aqueous extract (AE) of BXN were respectively subjected to pharmacodynamic and pharmacological comparison in assessing antidiarrheal effects with senna-induced diarrhea, castor oil-induced diarrhea, acetic acid-induced writhing assay, and isolated duodenum test. RESULTS: The highest yields of three detected components of BXN, rutaecarpine, eugenol and cinnamaldehyde were observed in EE. EE showed the most remarkable antidiarrheal activity in dose-dependent and time-dependent manners in both senna- and castor oil-induced diarrhea models, and presented dose-dependent analgesic activity in acetic acid-induced algesthesia model. In addition, EE extract of BXN also exhibited strong antimobility action on the intestine and strongest depression on spontaneous contraction of isolated duodenum. CONCLUSION: Ethanol extraction is an efficient method to extract the active constituents of BXN. BXN extract demonstrated multiple pharmacological activities affecting the main mechanisms of diarrhea, which validated BXN's usage in the comprehensive clinical treatment of diarrhea.

Scutellaria extract decreases the proportion of side population cells in a myeloma cell line by down-regulating the expression of ABCG2 protein.

BACKGROUND AND AIMS: Scutellaria is one of the most popular traditional Chinese herbal remedies against various human diseases, including cancer. In this study, we examined the active effects of Scutellaria extract and its main flavonoid constituents on the proportion of side population cells within human multiple myeloma cell line RPMI8226 in vitro and explored the potential molecular mechanisms involved. MATERIALS AND METHODS: The contents of flavonoids in ethanolic extract of Scutellaria baicalensis Georgi were determined using high performance liquid chromatography. The antiproliferative effect of the ethanolic extract on RPMI-8226 was determined by CCK assay. Apoptosis was measured by annexin combining with propidium iodide in a flow cytometer. Cell cycle analysis was performed by propidium iodide staining in combination with flow cytometry analysis. Hoechst 33342 exclusion assay was used for the identification of side population within RPMI8226 cells. The expression of ABCG2 protein was assessed by Western blotting assay. RESULTS: The content of major flavonoids constitutents of Scutellaria extract was baicalin (10.2%), wogonoside (2.50%), baicalein (2.29%), and wogonin (0.99%), respectively. The crude Scutellaria extract did not show significant anti-proliferative effect, apoptosis induction and cell cycle arrest in RPMI-8226 within the concentrations of 1-75µg/mL. However, the ethanolic extract, baicalein, wogonin and baicalin reduced the side population cells in RPMI-8226, and data showed that baicalein and wogonin had stronger inhibitory effects. Correspondingly, they also exhibited significant effects on decreasing the expression level of ABCG2 protein in RPMI-8226 in vitro. CONCLUSIONS: Our results for the first time demonstrated a novel mechanism of action for Scutellaria extract and its main active flavonoids, namely targeting SP cells by modulating the expression of ABCG2 protein. This study provides an insight for new therapeutic strategies targeting cancer stem cells of multiple myeloma.

Tanshinone IIA prevents uric acid nephropathy in rats through NF-κB inhibition.

The purpose of this research is to investigate the effect of tanshinone IIA, an extract of the Chinese medicine Que Xie Hua Yu Tang, on uric acid nephropathy (UAN) and to elucidate the underlying mechanisms. UAN rat model was established. Fifty UAN rats were randomly allocated into 5 groups: adenine-treated group, allopurinol-treated group, and low/middle/high dose of tanshinone IIA-treated groups. Meanwhile, another 10 rats were used as normal controls. Serum uric acid (UA), blood urea nitrogen (BUN), serum creatinine (Scr), MCP-1, and IL-1ß levels were measured. Histological staining was performed. Comparison between the adenine group and treatment (allopurinol and tanshinone IIA) groups showed compound treatment could attenuate the inflammation status of the kidneys and decrease serum UA levels. Among different kinds of medicine, tanshinone IIA had similar effects as allopurinol and exerted anti-inflammatory and renal protective effect in a dose-dependent manner. Furthermore, we found tanshinone IIA alone could also inhibit urate-induced MCP-1 and IL-1ß overexpression both in vivo and in vitro, accompanied with inhibition of NF-κB translocation from cytosome to nucleus. Tanshinone IIA could protect rats from uric acid-induced kidney damage, probably by attenuating renal inflammatory status.

[Clinical study on Shen Shuai Fang in treating chronic renal failure].

OBJECTIVE: To explore the effect of Shen Shuai Fang in treating Chronic Renal Failure (CRF) of deficiency of spleen and kidney with turbid damp and blood stagnation symptom. METHODS: 125 patients with CRF of deficiency of spleen and kidney with turbid damp and blood stagnation symptom were randomly divided into two groups, 75 patients in treatment group were treated with Shen Shuai Fang adding western medicine and the other patients in control group were treated only with western medicine. We observed the effect and indexes change including renal function, Hb, proteinuria, lipid before and after treatment. RESULTS: After six months' treatment, the general effective rate in treatment group was 77.33%, which was higher than that in control group obviously (44.00%, P < 0.01). So treatment group was obviously better than control group on decreasing proteinuria, improving renal function, increasing Hb, ameliorating lipid metabolism (P < 0.05 or P < 0.01). CONCLUSION: Shen Shuai Fang is effective to treat CRF of deficiency of spleen and kidney with turbid damp and blood stagnation symptom.

[A clinical research of Naoxinqing tablet's effects on blood fat and viscosity].

OBJECTIVE: To investigate the effects on blood fat and viscosity of Naoxinqing tablet. METHODS: 66 patients of primary hyperlipoidemia were randomly divided into three groups. The first group was treated by Naoxinqing tablet, the second group was treated by Naoxinqing and combined with half dose Simvastatin, the third group was treated by full dose Simvastatin. Then the clinical effect, fasting plasma lipid, the index of blood viscosity and side effect of the three group' patients were compared after 8 weeks. RESULTS: The index of blood fat improved obviously after treatment of the three groups ( P < 0.05, P < 0. 01), group 2 and 3 were significantly superior to group 1 (P < 0.05), and no significance between group 2 and 3 (P > 0.05). CONCLUSIONS: The Naoxinqing tablet can improve the metabolism of blood fat, decrease blood viscosity obviously and treat hyperlipoidemia effectively with little side effect.

[Clinical study on Dan Shao Tang in treating diabetic nephropathy of deficiency of Yin with damp-heat symptom].

OBJECTIVE: To explore the effect of Dan Shao Tang (DST) in treating diabetic nephropathy (DN) of deficiency of Yin with damp-heat symptom. METHODS: The 72 patients with DN of deficieny of Yin with damp-heat symptom were randomly divided into two groups. Among them, the 42 patients in treatment group were treated with DST and western medicine and the 30 patients in control group were treated only with western medicine. Before and after treatment, the effect and indexes change including blood glucose, renal function, proteinuria, microproteins, lipid metabolism and hyperviscosity were observed. RESULTS: After three months treatment, the general effective rate in treatment group was 76.19%, which was markedly higher than that in control group (50.00%, P < 0.01). Moreover, treatment group was obviously better than control group on decreasing blood glucose proteinuria and microproteins, improving renal function, ameliorating lipuid metabolism and hyperviscosity (P < 0.01 or P < 0.05). CONCLUSION: DST is effective ton treatment on DN of deficiency of Yin with damp-heat symptom.

[Effect of Shenqi Fuzheng injection (SFI) on immune function in patients with congestive heart failure].

OBJECTIVE: To study the effect of Shenqi Fuzheng injection on the humoral immunity (IgG IgM IgA), cellular immunity (T-lymphocyte subsets), Superoxide dismutase (SOD). Lpo and Plasma viscosity in the patients with congestive heart failure (CHF). METHODS: Sixty patients with CHF, whose heart function belonged to NYHA grade II-IV were randomly divide into two groups. The treaded group were treated with SFI 100 ml, and the control group were treated by nitroglycerine in jection 10 mg, the drug were administered respectively by adding in 5% glucose solution 500 ml for intravenous dripping, once a day, 20 days as one therapeutic course. Venous blood from cubital vein was collected before and after treatment to detect the IgG, IgM, IgA, T-lymphocyte subsets, SOD, LPO and Plasma viscosity. RESULTS: The clinical heart function markedly improved rate and total effective rate in the treated group was singificantly better than those in the control group respectively (P < 0.05). the left ventricular ejecting frection (LVEF) and end syctolic volume (ESV) were improved in both group (P < 0.05, P < 0.01), and the improvement in the treated group was superior to that in the control group (P < 0.05). In the treated group after treatment, the CD4, SOD level and CD4/DC8 ratio increased (P < 0.05), level of LPO, IgG and IgM lowered (P < 0.05) significantly, while those in the control group were not changed singificantly (P > 0.05). Plasma viscosity of treatment group also got better improved than before (P < 0.05), and there was a significantly difference between the two groups after treatment (P < 0.05). CONCLUSION: SFI Can improve the immune funtion of CHF patients, and can be taken as an importmant auxiliary treatment for CHF.

[Clinical observation of "dan shao tang" in treating mesangial proliferative glomerulonephritis of deficiency of yin with damp-heat symptom].

OBJECTIVE: To explore the effect of "Dan Shao Tang" (DST) on mesangial proliferative glomerulonephritis (MsPGN) of deficiency of Yin with damp-heat symptom. METHODS: 90 patients with MsPGN of deficiency of Yin with damp-heat symptom were randomly divided into two groups. 54 patients in treatment group were treated with DST and western medicine, and 36 patients in control group were treated only with western medicine. We observed the effect and indexes change including renal function, proteinuria, hematuria, lipid before and after treatment. RESULTS: After three months treatment, the general effective rate in treatment group was 75.93%, which was markedly higher than that in control group (47.22%, P < 0.01). Treatment group is obviously better than control group on decreasing proteinuria and hematuria, improving renal function, ameliorating lipid metabolism (P < 0.05). CONCLUSION: DST is effective on MsPGN of deficiency of Yin with damp-heat symptom.

[Clinical study of zhuyutongmai capsule on treating senile cerebral infarction and effect on function of vascular endothelium].

OBJECTIVE: To evaluate the clinical study of Zhuyutongmai (ZYTM) Capsule in treating senile cerebral infarction and effect on function of vascular endothelium. METHODS: Sixty-four aged patients with cerebral infarction were randomly divided into the treated group treated with ZYTM and the control group treaded with Naoluotong. The clinical effect was observed. The hemorrheologic and hemodynamic parameters, the blood levels of endothelin (ET) and nitric oxide (NO) were determined before and after treatment. RESULTS: After treatment of ZYTM, the hemorrheologic and hemodynamic parameters were all significantly improved (P < 0.01 or P < 0.05), the therapeutic effect of ZYTM was better than that of Naoluotong (P < 0.05), compared with the control group after treatment, the level of ET in the treated group were lower (P < 0.01), and level of NO were higher (P < 0.01). CONCLUSION: ZYTM has good effect in treating senile cerebral infarction.

[Clinical study on dan shao tang in treating IgA nephropathy of deficiency of yin with damp-heat symptom].

OBJECTIVE: To explore the effect of Dan Shao Tang(DST) in treating IgA nephropathy(IgAN) of deficiency of Yin with damp-heat symptom. METHODS: 90 patients with IgAN of deficiency of Yin with damp-heat symptom were randomly divided into two groups. 50 patients in treatment group were treated with DST and western medicine and 40 patients in control group were treated only with western medicine. The effects and changes of the indexes including renal function, hematuria, proteinuria, blood IgA before and after treatment were observed. RESULTS: After six months treatment, the general effective rate in treatment group was 70.00%, which was markedly higher than that in control group(37.50%, P < 0.01). Treatment group is obviously better than control group on decreasing hematuria, proteinuria, blood IgA and improving renal function(P < 0.05). CONCLUSION: DST is effective on IgAN of deficiency of Yin with damp-heat symptom.

[Clinical observation of wenshen xiezhuo decoction in treating pations with chronic renal failure].

OBJECTIVE: To observe curative effect of Wenshen Xiezhuo Decoction (WSXZD) in treating pations with chronic renal failure (CRF). METHODS: Sixty patients with CRF of deficienncy of spleen-Yang and kidney-Yang with damp pathogen and blood stasis symptom were randomly divided into the treated group and the control group, 30 in each group. Both groups were treated with low-protein diets, controlling hypertension and symptomatic treatment. And WSXZD was given additionally to the treated group. The curative effect and the changes of integral of traditional Chinese medicine (TCM) symptoms, renal function, serum calcium, serum phosphorus and ALB were observed after two months. RESULTS: The significant and total effective rate of the treated group were higher than that of the control group (P < 0.05, P < 0.01). Compared with the control group, integral of TCM symptoms and the levels of serum phosphorus were decreased significantly, the levels of ALB were increased significantly, and renal function was improved significantly in the treated group (P < 0.05, P < 0.01). CONCLUSION: The curative effect of conventional western medicine therapy plus WSXZD in treating pations with CRF of deficiency of spleen-Yang and kidney-Yang with damp pathogen and blood stasis symptom was significantly better than that of conventional western medicine therapy.

[The clinical study on Dan Shao Tang in treating simple hematuria of masked nephritis of deficiency of Yin with damp-heat symptom].

OBJECTIVE: To explore the effect of Dan Shao Tang (DST) in treating simple hematuria of masked nephritis of deficiency of Yin with damp-heat symptom. METHODS: Sixty patients of simple hematuria of masked nephritis of deficiency of Yin with damp-heat symptom were divided into two groups in random, 35 patients in treatment group were treated with Dan Shao Tang and westen medicine, and 25 patients in control group were treated only with western medicine. The effect, change of hematuria and immunity index of the two groups were observed three months later. RESULTS: The total efficiency of treatment group (65.71%) is better than that of control group (36%). Treatment group is obviously better than control group on decreasing hematuria and improving immune function. CONCLUSION: Dan Shao Tang is effective to treatment on simple hematuria of masked nephritis of dificiency of Yin with damp-heat symptom effectively.

[Clinical observation on colquhounia root tablet in treating lipid metabolism disturbance secondary to nephrotic syndrome].

OBJECTIVE: To study the effect of Colquhounia root tablet (CRT) in treating nephrotic syndrome with sequential lipid metabolism disorder (NS-LMD). METHODS: The 96 patients with NS-LMD were randomly divided into two groups, the 60 cases in the treated group treated with CRT and the 36 cases in the control group treated with hormone or cytotoxic medicine. The curative effect and the related indexes were determined before and after treatment. RESULTS: After six months treatment, the general effective rate in the treated group was 88. 33%, which was markedly higher than that in the control group (69.44%, P < 0.05). The levels of the treated group in ameliorating lipid metabolism disorder and renal dysfunction were also higher than those in the control group (P < 0.05). CONCLUSION: CRT could improve NS-LMD, improve renal function, eliminate urinary protein and increase plasma albumin. It is highly effective with low toxicity and safe.

[Clinical study of treatment on chronic uric acid nephropathy by integrating Western and traditional Chinese medicine].

OBJECTIVE: To investigate the ameliorative effect on chronic uric acid nephropathy (CUAN) by integrating western and traditional Chinese medicine (IWTCM). METHODS: The 136 CUAN patients were divided into two groups at random, the therapy group of 86 patients were treated by Chinese medicine and allopurinol, and the control group of 50 patients were treated only by allopurinol. The curative effect and the related index such as blood uric acid, renal function, urinary protein, microproteins, blood lipid and hyperviscosity were determined before and after being treated. RESULTS: After three months treatment, the total effective rate in the therapy group (90.7%) was significantly higher (P < 0.01) than that of the control group (56%). The therapy group is also superior to the control group in improving renal function, lipid metabolism and hyperviscosity, decreasing blood uric acid, urinary protein, microproteins in evidence (P < 0.05, P < 0.01). CONCLUSION: IWTCM can obviously improve the ameliorative effect on chronic uric acid nephropathy.