RESUMEN
Affinity chromatography is characterized by its high specificity,high recovery rate and sensitivity,and it has been widely used in the selection of active ingredients of traditional Chinese medicine,separation and enrichment of low molecular weight sugars and protein peptides,research on mechanism of action and discovery of targets.This paper reviewed the application of affinity chromatography and its adsorption isotherm model,kinetic model and adsorption thermodynamic mechanism in the field of traditional Chinese medicine.This summarizes and provides thinking for comprehensive applications of affinity chromatography theory in the field of active ingredient screening,purification and medicine interaction.
Asunto(s)
Adsorción , Cromatografía de Afinidad , Medicamentos Herbarios Chinos , Química , Medicina Tradicional China , Modelos TeóricosRESUMEN
Linalool, as a major volatile compound, is widely distributed in natural plant essential oil. In addition, it can also be artificially synthesized. Linalool is used frequently as an important ingredient of perfumes and household detergents. It is still employed in food flavor and industries. Besides, linalool has some positive effect on healthcare. Many studies have showed that linalool exhibited a variety of pharmacological activities, including analgesic, anxiolytic, sedative, anti-inflammatory, anti-tumor and anti-bacterial effects. Therefore, linalool will be a promising agent for clinical application. This article reviews the pharmacological effects and formulation studies of linalool so as to provide a theoretical basis for its further development and utilization.
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Animales , Humanos , Ansiolíticos , Química , Farmacología , Antiinflamatorios , Química , Farmacología , Química Farmacéutica , Medicamentos Herbarios Chinos , Química , Farmacología , Hipnóticos y Sedantes , Química , Farmacología , Monoterpenos , Química , FarmacologíaRESUMEN
Affinity chromatography is a chromatographic method for separating molecules using the binding characteristics of the stationary phase with potential drug molecules. This method can be performed as a high throughput screening method and a chromatographic separation method to screen a variety of active drugs. This paper summarizes the history of affinity chromatography, screening technology of affinity chromatography, and application of affinity chromatography in screening bio-active compounds in herbal medicines, and then discusses its application prospects, in order to broaden applications of the affinity chromatography in drug screening.
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Animales , Humanos , Cromatografía de Afinidad , Métodos , Evaluación Preclínica de Medicamentos , Métodos , Medicamentos Herbarios Chinos , Química , Farmacología , Plantas Medicinales , QuímicaRESUMEN
Recent years, chemical constituents from spice volatile oils have gained worldwide concern owing to its multiple pharmacological effects and safety for using as the natural antibacterial agents. However, their poor dissolution, strong volatility, serious irritation, weak stability, easy oxidation and low bioavailability characteristics are the major obstacle in the preparation of effective oral formulation and practical application. Therefore, there is an urgent need to select a novel carrier system that can delivery the chemical constituents from spice volatile oils more efficiently with improving their stability as well as alleviating the irritation, and develop the functional food, health products and even medicine for exerting their pharmacological effects, which also is the focus and nodus of the research on their application. This review presents recent systematic studies on their novel carrier systems, including cyclodextrin inclusion complex, liposomes, nanoemulsions, nanoparticles, solid dispersion and so on, and summarizes the characteristics, application range and problems of each novel carrier systems, in order to provide some beneficial thoughts in further developing new products of chemical constituents from spice volatile oils.
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Química Farmacéutica , Métodos , Portadores de Fármacos , Química , Nanopartículas , Química , Aceites Volátiles , Química , Extractos Vegetales , Química , EspeciasRESUMEN
Bufalin is an active compound of the traditional Chinese medicine Chansu, which exhibits significant anti-tumor activities in many solid tumors and leukemia cell lines. Bufalin could introduce apoptosis, reverse drug-resistance, and prevent migration and invasion of tumor cells. This paper reviewed the latest research progress of the in vitro and in vivo anti-tumor effect and mechanism of bufalin on a series of cancers, such as hepatocellular carcinoma, lung cancer, colon cancer, gastric cancer, leukemia, bladder cancer, and its formulation study is also summarized for the reference of its further study and application.
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Animales , Antineoplásicos , Química , Farmacología , Usos Terapéuticos , Bufanólidos , Química , Farmacología , Usos Terapéuticos , Química Farmacéutica , Métodos , Neoplasias , Quimioterapia , PatologíaRESUMEN
As many traditional Chinese medicines have been founded to have protective effect on liver damage in recent years, they have also got involved in increasingly wide clinical application. Meanwhile, with the development of new hepatic protective formulations of traditional Chinese medicines, we have set increasingly higher requirements for quality control methods and measures. This essay summarizes the advance in studies on hepatic protective formulations of traditional Chinese medicine and their quality control methods in the combination of relevant domestic and foreign literatures, looking into the future of the development of new hepatic protective formulations of traditional Chinese medicines.
Asunto(s)
Animales , Humanos , Química Farmacéutica , Medicamentos Herbarios Chinos , Estándares de Referencia , Usos Terapéuticos , Hígado , Hepatopatías , Quimioterapia , Medicina Tradicional China , Estándares de Referencia , Fitoterapia , Estándares de Referencia , Control de CalidadRESUMEN
Nanocarriers generally made of natural or artificial polymers ranging in size from about 10-1 000 nm, possess versatile properties suitable for drug delivery, including good biocompatibility and biodegradability, potential capability of targeted delivery and controlled release of incorporated drugs, and have been extensively used in the development of new drug delivery systems (DDS). These types of nano-DDS have considerable potential to traditional Chinese medicine (TCM), and recently have attracted increasing efforts on the TCM research and development. In this review, the recently published literature worldwide is covered to describe the latest advances in the applications as TCM delivery carriers, and to highlight the characteristics and preparation methods of some selected examples of promising nanocarriers such as nanoparticles, lipid nanoparticles, nanoemulsions, nanomicelles and nanoliposomes.
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Portadores de Fármacos , Química , Medicina Tradicional China , Métodos , Nanoestructuras , Química , Nanotecnología , MétodosRESUMEN
<p><b>OBJECTIVE</b>To determine the encapsulation efficiency of silybin liposomes.</p><p><b>METHOD</b>After Silybin liposomes solution was separated through sphadex G-100 column, eluent collected was determined by the first derivative UV spectrometry.</p><p><b>RESULT</b>The calibration curve of Silybin was linear in the range of 0.5-30.0 mg x L(-1) (r = 0.9998). The average recovery was 101.7%. The relative standard deviation was 1.8%. The results showed that the encapsulation efficiency ranged from 65.1%-83.0% when the drug/phospholipid ratio varied from 0.02-0.06. The concentration of SLB in liposomes prepared by optimum technique was 760.4 mg x L(-1) (n = 4).</p><p><b>CONCLUSION</b>The method is accurate, simple and suitable for the quality control of Silybin liposomes.</p>