RESUMEN
In the present study, a simple and efficient method for the preparative separation of 3-CQA from the extract of Helianthus tuberosus leaves with macroporous resins was studied. ADS-21 showed much higher adsorption capacity and better adsorption/desorption properties for 3-CQA among the tested resins. The adsorption of 3-CQA on ADS-21 resin at 25°C was fitted best to the Langmuir isotherm model and pseudo-second-order kinetic model. Dynamic adsorption/desorption experiments were carried out in a glass column packed with ADS-21 to optimise the separation process of 3-CQA from H. tuberosus leaves extract. After one treatment with ADS-21, the content of 3-CQA in the product was increased 5.42-fold, from 12.0% to 65.2%, with a recovery yield of 89.4%. The results demonstrated that the method was suitable for large-scale separation and manufacture of 3-CQA from H. tuberosus leaves.
Asunto(s)
Ácido Clorogénico/aislamiento & purificación , Cromatografía/métodos , Helianthus/química , Extractos Vegetales/aislamiento & purificación , Resinas Sintéticas/química , Adsorción , Ácido Clorogénico/análisis , Cromatografía/instrumentación , Cinética , Extractos Vegetales/análisis , Hojas de la Planta/químicaRESUMEN
Two new flavonoid glycosides, named quercetin-3-O-(2â³-O-tigloyl)-α-L-rhamnopyranoside (1) and quercetin-3-O-(3â³-O-tigloyl)-α-L-rhamnopyranoside (2), together with 10 known flavonoids (3-12), were isolated from the whole plant of the halophyte Limonium franchetii. Their structures were elucidated on the basis of extensive spectroscopic analysis including 2D NMR and HR-EI-MS. In addition, primary bioassays showed that compound 1 had moderate cytotoxic activity against rat C6 glioma cell lines.
Asunto(s)
Medicamentos Herbarios Chinos/aislamiento & purificación , Glicósidos/aislamiento & purificación , Plumbaginaceae/química , Quercetina/análogos & derivados , Quercetina/aislamiento & purificación , Animales , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Glioma/tratamiento farmacológico , Glicósidos/química , Glicósidos/farmacología , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Quercetina/química , Quercetina/farmacología , RatasRESUMEN
Eleven flavonoids were isolated from the leaves of the halophyte Apocynum venetum. Among them, the isolation of plumbocatechin A (1), 8-O-methylretusin (2) and kaempferol 3-O-(6â³-O-acetyl)-ß-D-galactopyranoside (7) was reported for the first time from this plant. Their structures were identified by using spectral methods, including 2D NMR experiments, and confirmed by comparing with the literature data. In addition, the antifouling activities of these compounds against the marine fouling bacteria, Bacillus thuringiensis, Pseudoalteromonas elyakovii and Pseudomonas aeruginosa, have been evaluated in this article.