A comprehensive review on the ethnobotany, phytochemistry, pharmacology and quality control of the genus Lycium in China.

The Lycium genus, perennial herbs of the Solanaceae family, has been an important source of medicines and nutrient supplements for thousands of years in China, where seven species and three varieties are cultivated. Among these, Lycium barbarum L. and Lycium chinense Mill., two "superfoods", together with Lycium ruthenicum Murr, have been extensively commercialized and studied for their health-related properties. The dried ripe fruits of the genus Lycium are well recognized as functional foods for the management of various ailments including waist and knee pain, tinnitus, impotence, spermatorrhea, blood deficiency and weak eyes since ancient times. Phytochemical studies have reported numerous chemical components in the Lycium genus, categorized as polysaccharides, carotenoids, polyphenols, phenolic acids, flavonoids, alkaloids and fatty acids, and its therapeutic roles in antioxidation, immunomodulation, antitumor treatment, hepatoprotection and neuroprotection have been further confirmed by modern pharmacological studies. As a multi-functional food, the quality control of Lycium fruits has also attracted attention internationally. Despite its popularity in research, limited systematic and comprehensive information has been provided on the Lycium genus. Therefore, herein, we provide an up-to-date review of the distribution, botanical features, phytochemistry, pharmacology and quality control of the Lycium genus in China, which will provide evidence for further in-depth exploration and comprehensive utilization of Lycium, especially its fruits and active ingredients in the healthcare field.

Chemical characterization and 5α-reductase inhibitory activity of phenolic compounds in goji berries.

Lycium fruits have a high content of phenolics as bioactive constituents with various pharmacological effects, but there is a lack of comparative analysis and chemical profiling of phenolics in Lycium fruit varieties. An ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MSE) combined with chemometrics was developed to characterize the phenolics in fruits from four Lycium species, including Lycium barbarum L. (LBL), L. chinense Mill. (LCM), L. barbarum var. auranticarpum (LBA) and L. ruthenicun Murr. (LRM). 63 phenolics were identified according to reported tandem mass fragmentation patterns and the UNIFI scientific informatics platform. Nine phenolics (5, 18, 20, 29, 31, 37, 41, 43, 60) were common and predominant components among four types of Lycium fruit. The partial least squares discriminant analysis (PLS-DA) and the orthogonal partial least squares discriminant analysis (OPLS-DA) were analyzed on the basis of a matrix created from 653 sets of data, and 20 Lycium fruits were classified into four groups. Further analysis identified that phenolics profiles were characteristic for each Lycium species, and five markers (13, 29, 31, 35, 99) could be utilized for fruit identification. Subsequently, inhibitory activity against 5α-reductase of phenolic extracts of Lycium fruits showed that LBL extract was the relative better effective, followed by LCM, whereas LBA and LRM showed no activity, which might be associated with the high contents of marker compounds (29, 31, 35, 43, 71, 99) in LBL. These findings will provide guidance for the development of Lycium phenolics with beneficial properties for the prevention and treatment of Benign prostatic hyperplasia (BPH).