RESUMEN
<p><b>OBJECTIVE</b>To study the gene expression profiles in kidney Yang deficiency in individuals with sub-health status.</p><p><b>METHODS</b>The subjects identified to have Kidney-yang deficiency and sub-health status were enrolled in this study with healthy subjects as the normal control. Using gene chip technique and MAS software, the gene expression profiles in kidney Yang deficiency in the subjects with sub-health status were analyzed.</p><p><b>RESULTS</b>Characteristic profiles of the differentially expressed genes were found between the kidney Yang deficiency group and normal control group. Among the total of 75 differentially expressed genes, 22 were up-regulated (with a ratio greater than 2) and 53 were down-regulated (with a ratio less than 0.5) in kidney Yang deficiency group.</p><p><b>CONCLUSION</b>Gene chip technique allows rapid and high-throughput screening for gene expression in kidney Yang deficiency and sub-health status, and a initial evidence of kidney Yang deficiency-related genes has been obtained.</p>
Asunto(s)
Adulto , Femenino , Humanos , Masculino , Diagnóstico Diferencial , Expresión Génica , Perfilación de la Expresión Génica , Estado de Salud , Enfermedades Renales , Diagnóstico , Medicina Tradicional China , Métodos , Análisis de Secuencia por Matrices de Oligonucleótidos , Transcriptoma , Deficiencia Yang , Diagnóstico , GenéticaRESUMEN
<p><b>OBJECTIVE</b>To evaluate the reliability and validity of the questionnaire we designed for subhealth status survey based on the symptoms described in traditional Chinese medicine.</p><p><b>METHOD</b>By on-the-spot investigation, the recovery rate of the questionnaires was 96.3% and the response rate of the items was 97.2%.</p><p><b>RESULTS</b>Internal consistency reliability coefficient of the questionnaire was high (0.9274-0.9676), and each item was closely associated with its related factors (with Spearman coefficient mostly above 0.6 except for that for bodily symptoms in female). Factor loading was approximately consistent with the structure and content of the questionnaire.</p><p><b>CONCLUSION</b>This subhealth status questionnaire can be reliable and effective.</p>