Efficacy evaluation of acupoints massage on asthenopia of video display terminal under different exposure dose / 中国针灸

<p><b>OBJECTIVE</b>To observe the efficacy differences of acupoints massage for asthenopia of video display terminal (VDT) under different exposure dose.</p><p><b>METHODS</b>One hundred and two cases (204 eyes) were divided into a low exposure group and a high exposure group, fifty-one cases in each group. The same intervention of acupoints massage on Cuanzhu (BL 2), Jingming (BL 1), Sizhukong (TE 23), Sibai (ST 2) and Taiyang(EX-HN 5) were given to the two groups, one acupoint for 5 min and once everyday, one month of which made a course. The symptom score, tear film break-up time (BUT) and Schirmer I test(SIT) were observed before and after treatment.</p><p><b>RESULTS</b>(1) The correlation coefficient of cubic curve model of the exposure dose was the biggest with symptom improvement index (P = 0.000), which indicated that the lower VDT exposure index was, the more obvious the symptom improved. The symptom improvement indices of low exposure group and high exposure group, which were (52.31 +/- 16.65)% and (28.93 +/- 13.35)% respectively, were statistical significant difference (P = 0.000). (2) Compared to before treatment, the levels of BUT and SIT in the two groups were both significantly higher (P < 0.05). Compared with the high exposure group, the levels of BUT and SIT in the low exposure group were increased by 0.826 s (P = 0.022) and 1.029 mm (P = 0.033), respectively, after the impact of BUT and SIT was corrected before the research.</p><p><b>CONCLUSION</b>The acupoints massage can improve the symptoms and ocular physiology for patients with VDT asthenpia, and it is more effective for the low exposure cases.</p>

Anticancer effect of 5-fluorouracil combined with extract of Rosa roxburghii Tratt on human endometrial adenocarcinoma / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate anticancer effects of 5-fluorouracil (5-FU) combined with CL, extract of Rosa roxburghii Tratt on human endometrial adenocarcinoma cell line (JEC).</p><p><b>METHODS</b>JEC cells cultured in vitro in the logarithmic growth phase were seeded in the culture plate and divided into the control group (RPMI 1640), the positive group (10(-4) mol/L 5-FU), the CL groups (at the dose of 0.01, 0.1, 1, 10, and 100 microg/mL), and the CL (0.01, 0.1, 1, 10, and 100 microg/mL) combined with 5-FU groups. Effects of 5-FU combined with CL on JEC cell growth were drawn and measured by MTT and growth curves. Effects of CL combined with 5-FU on the JEC cell differentiation was analyzed by detecting the reduction capability of nitrobenzene thiocyanate (NBT) and lactate dehydrogenase (LDH) contents in the cultured medium. Effects of CL combined with 5-FU on the JEC cell apoptosis and cell proliferation cycle were detected by acridine orange (AO)/ethidium bromide (EB) fluorescent staining and flow cytometry (FCM).</p><p><b>RESULTS</b>The proliferation inhibitory effect of CL combined with 5-FU on JEC cells was enhanced when compared with that of CL or 5-FU alone (P<0.05). The percentages of NBT positive JEC cells and apoptotic JEC cells increased in the 5-FU combined with CL groups when compared with 5-FU group or the CL group alone (P<0.05). The LDH concentration of the JEC cell culture supernate decreased in 5-FU combined with CL groups (P<0.05). Furthermore, the percentage of G0-G1 phase JEC cells treated by 5-FU combined with CL was higher than that of 5-FU or CL alone (P<0.05).</p><p><b>CONCLUSION</b>CL could enhance anticancer effects of 5-FU. Its mechanisms might be correlated with reinforcing the cytotoxicity of 5-FU, inducing cell differentiation and apoptosis, and inhibiting cell proliferation and division.</p>

Anticancer effect of CL extract of Rosa roxburghii / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate anticancer effects and potential mechanisms of CL, extract of Rosa roxburghii.</p><p><b>METHOD</b>In vitro anticancer effect was observed in Ehrlich's ascites carcinoma (EAC) mice model. Cell toxicity of CL on human endometrial adenocarcinoma cell line JEC (JEC) cells was measured by MTT reduction test and growth curves drawing by trypan blue dye exclusion method. Lactate dehydrogenase (LDH) concentration of cultured medium was detected by auto-biochemistry-meter. Cell differentiation was showed by detection of NBT reduction ability. Apoptosis was showed by AO/EB fluorescent staining and flow cytometer detection. Cell proliferation cycle was detected by flow cytometer.</p><p><b>RESULT</b>Comparing with the negative group, life span of EAC mice treated with CL was prolonged (P <0.05), and thymus index and spleen index of them were raised (P <0.05). The inhibitory effect of CL on JEC cells was in concentration-and time-dependent manner. IC50 of CL on JEC cells was 0.05 microg mL(-1) in 96 hours. Growth curves showed right-shift with CL concentration increasing. The number of NBT positive JEC cells increased and the LDH concentration of cultured medium declined with CL increasing. Apoptosis of JEC cells with CL treated was induced in concentration-dependent manner, apoptotic percentage of CL 10 microg mL(-1) on JEC cells was 25.59% in 24 hours. CL arrested JEC cells in G2-M phase (P <0.05).</p><p><b>CONCLUSION</b>CL has certainly anticancer effects in vivo and in vitro. Anticancer effect of CL in vivo was in relation to enhancing immune function of EAC mice; anticancer mechanisms of CL on JEC cells may be its direct cytotoxic effect, inducing cell apoptosis and inhibiting cell segmentation.</p>

Two new flavones from Fordia cauliflora of Yunnan / 药学学报

<p><b>AIM</b>To study the chemical constituents of the stem of Fordia cauliflora of Yunnan province.</p><p><b>METHODS</b>The constituents were separated and purified by repeated silica column chromatography. The structures were elucidated by physical-chemical properties and spectroscopic data.</p><p><b>RESULTS</b>Six compounds were isolated from the ethanol extract of the stem of Fordia cauliflora. They were identified as: 6-hydroxy-3-methoxy-6",6"-dimethylchromeno-(2", 3" : 7, 8)-flavone (1), 3-methoxy-6-(3-methyl-but-2-enyloxy)-6", 6"-dimethylchromeno-( 2", 3" : 7, 8)-flavone (2), 3, 6-dimethoxy-6", 6"-dimethylchromeno-( 2", 3" : 7, 8)-flavone (3), 7-hydroxy-4'-methoxyisoflavone (4), 7, 4'-dihydroxyisoflavone (5) and karanjin (6).</p><p><b>CONCLUSION</b>Compounds 1 and 2 are new compounds. Compounds 3 -5 were isolated from the plant for the first time.</p>

Chemical constituents from Viscum nudum and their accelerating PC12 cell differentiation / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate chemical constituents of the stem of Viscum nudum and their bioacyivity.</p><p><b>METHOD</b>The major chemical constituents were isolated from the AcOEt-solved part of ethanol-extract of the plant by column chromatography and the active screening test in vitro were taken out for looking for compounds to acccelerate PC12 cell differentiation.</p><p><b>RESULT</b>5 compounds were identified as eriodictyol (1), 5, 7-dihydroxy-3', 4'-dimethoxy flavanone (2), oleanolic (3), 5, 7-dihydroxychromone (4) and homeriodictyol (5) by spectral evidences, in which homeriodictyol (5) had acceleration differentiation to PC12 cell.</p><p><b>CONCLUSION</b>All compounds were obtained from this plant for the first time, and bioactive constituent was observed in the AcOEt-solved part.</p>

Chemical composition of the volatile oil from Cynanchum stauntonii and its activities of anti-influenza virus.

The volatile oil of the roots of Cynanchum stauntonii was examined by gas chromatography-mass spectrometry (GC-MS). Thirty-eight constituents were identified. (E,E)-2,4-Decadienal, 3-efhyl-4-methypentanol, 5-pentyl-3H-furan-2-one, (E,Z)-2,4-decadienal and 2(3H)-furanone,dihydro-5-pentyl were found to be the major components. The volatile oil exhibited the activities against influenza virus in vitro (IC50s=64 microg/ml). In in vivo experiment, it prevented influenza virus-induced deaths in a dose-dependent manner.

Studies on chemical constituents from the root of Mirablis jalapa / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents from the root of Mirabilis jalapa.</p><p><b>METHOD</b>Compounds were isolated from 75% ethanolic extract of the titled herb by silica gel column chromatography, and their structures were elucidated by physical and chemical evidences and spectroscopic analysis.</p><p><b>RESULT</b>Four compounds were obtained and identified as (2, 5-dioxoimidazolidin-4-yl)-urea (1), glycerin monoeicosate (2), boeravinone (3) and beta-sitosterol (4).</p><p><b>CONCLUSION</b>Compound (2) is a new compound, and compound (1) was obtained from this plant for the first time.</p>

Analysis of 1H-NMR fingerprint in stem of Dendrobium loddigesii / 中国中药杂志

<p><b>OBJECTIVE</b>To analyse the 1H-NMR finger-print of the stem of Dendrobium loddigesii.</p><p><b>METHOD</b>Silica gel column chromatography was used to separate the chemical constituents of SCE A of the stem of D. loddigesii. The characteristic signals of the H-NMR finger-print were analysed after determining the structures of the compounds isolated from SCE A.</p><p><b>RESULT</b>1H-NMR finger-prints of the samples of D. loddigesii collected from different regions showed highly characteristic features and reproducibility. Four compounds predominant in SCE A were isolated and their structures were determined by spectral analysis as 1, 2, 3 and 4, respectively.</p><p><b>CONCLUSION</b>Compound 3 and 4 were isolated from D. loddigesii for the first time. The 1H-NMR finger-print of CGE A of the stem of D. loddigesii showed mainly the characteristic signals of the above four compounds and might be utilized for the original authentication of this plant.</p>