RESUMEN
Herein, we presented two novel turn-on colorimetric and fluorescent probes based on a F- triggered SiO bond cleavage reaction, which displayed several desired properties for the quantitative detection for F-, such as high specificity, rapid response time (within 3â¯min) and naked-eye visualization. The fluorescence intensity at 574â¯nm (absorbance at 544â¯nm) of the solution was found to increase linearly with the concentration of F- (0.00-30.0⯵M) with the detection limit was estimated to be 0.47⯵M/0.48⯵M. Based on these excellent optical properties, the probes were employed to monitor F- in real water samples and tea samples with satisfactory. Furthermore, it was successfully applied for fluorescent imaging of F- in living nude mice, suggesting that it could be used as a powerful tool to predict and explore the biological functions of F- in physiological and pathological processes.
Asunto(s)
Colorantes Fluorescentes/química , Fluoruros/análisis , Imagen Óptica/métodos , Espectrometría de Fluorescencia/métodos , Xantonas/química , Animales , Colorimetría , Límite de Detección , Modelos Lineales , Ratones , Ratones Desnudos , Té/química , Agua/químicaRESUMEN
One new phenanthrene derivative phoimbrtol A (1) with seven known compounds, loddigesiinol B (2), shanciol B (3), (-)-medioresinol (4), (-)-pinoresinol (5), quercetin 3-O-ß-L-arabinofuranoside (6), luteolin 7-O-ß-glucoside (7) and platycaryanin D (8) have been isolated from the ethyl acetate extract of the air-dried whole plant of Pholidota imbricata Hook. Their inhibitory effects on nitric oxide (NO) production and 1,1-diphenyl-2-picrylhydrazil (DPPH) radical scavenging activity were examined. Among these compounds, 8 exhibited the most potent activity at NO production inhibitory assay and DPPH radical scavenging assay, stronger than those of the familiar antioxidative agents, quercetin and resveratrol.
Asunto(s)
Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Depuradores de Radicales Libres/aislamiento & purificación , Depuradores de Radicales Libres/farmacología , Orchidaceae/química , Fenantrenos/aislamiento & purificación , Fenantrenos/farmacología , Animales , Antioxidantes/química , Compuestos de Bifenilo/farmacología , Medicamentos Herbarios Chinos/química , Flavonoides/química , Depuradores de Radicales Libres/química , Macrófagos/efectos de los fármacos , Ratones , Óxido Nítrico/biosíntesis , Resonancia Magnética Nuclear Biomolecular , Fenantrenos/química , Picratos/farmacología , Quercetina/farmacología , Resveratrol , Estilbenos/farmacologíaRESUMEN
A metabonomic approach based on ultra-performance liquid chromatography coupled to mass spectrometry (UPLC/MS) was used to study the nephrotoxicity of rhizoma alismatis (RA) in rats. Potential biomarkers of RA toxicity were identified and the toxicological mechanism is discussed. Urine samples were collected from control and treated rats at various stages and analyzed by UPLC/MS in positive ionization mode. Histopathological analysis was used to evaluate renal function. The differences in the metabolic profiles of the control and treated rats were clearly distinguishable with principal components analysis (PCA) of the chromatographic data, and significant changes in 13 metabolite biomarkers were detected in the urine. This metabonomic method combined with PCA could discriminate the treated rats from the control rats on days 60, 120, and 180 after treatment, before serious organic renal damage was apparent on day 180 with histopathology. This research indicates that UPLC/MS-based metabonomic analysis of urine samples can be used to predict the chronic nephrotoxicity induced by rhizoma alismatis.