RESUMEN
Plants from different functional groups show distinct response to nitrogen (N) addition, however, the patterns and underlying mechanisms across the natural N availability gradient remain unexplored. In present studies, effects of soil N fertility on aboveground N pools and production were examined, and the N: P stoichiometry was compared among forbs, graminoids, and legumes on an alpine grassland located in northeast of Qinghai-Tibet Plateau. Mean N: P ratios of the whole community in 2008 and 2009 were 9. 83 and 11.57, respectively, indicating that the sub-alpine grassland was mainly N-limited. The partial redundancy analysis (partial RDA) showed that the biomass percentage of forbs increased, while those of legumes and graminoids decreased with the soil N availability. It suggested that legumes and graminoids were more competitive than forbs in the natural N-limited grassland, which may be due to the N2-fixation ability and high nutrient use efficiency, respectively. Under the projected increasing N deposition, the vegetation tended to shift from graminoids and legumes to forbs. Significant relationships were found between the percentage of legumes biomass and N: P (phosphorus) ratio for the whole community and non-legumes, indicating that legumes could improve the community N status as well as non-legumes N status on the alpine grassland.