RESUMEN
Caffeine has known effects on the central nervous and cardiovascular systems. An intake up to 400 mg/day does not give rise to health concerns. Tea, a major source of caffeine, is highly consumed in China. However, the potential health risk of caffeine from tea has not been well evaluated. The present study assessed caffeine intake levels from tea for Chinese adult consumers. We collected 1,398 samples of green, black, dark, jasmine, oolong, white, and yellow tea from 17 provinces. The caffeine content was determined by HPLC. The average contents were 27 (oolong tea) - 43 (yellow tea) mg/g. The leaching rate of caffeine into the water was about 100%. Tea consumption data were from the National Beverage Consumption Survey 2013-2014. Monte Carlo simulations were applied to estimate the distribution of caffeine intake. The average caffeine intake from tea was 180 mg/day of all consumers. Green, dark, and black tea were the primary sources. Males (197 mg/day) consumed more caffeine than females (136 mg/day) on average, but females older than 71 years had the highest intake level (259 mg/day) among all subgroups. Over 90% of Chinese adult tea drinkers have caffeine intake under 400 mg/day.
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Cafeína , Camellia sinensis , Adulto , Bebidas , Cafeína/análisis , Café , Femenino , Humanos , Masculino , TéRESUMEN
Inflammation and neuronal apoptosis aggravate the secondary damage after spinal cord injury (SCI). Rehmannioside A (Rea) is a bioactive herbal extract isolated from Rehmanniae radix with low toxicity and neuroprotection effects. Rea treatment inhibited the release of pro-inflammatory mediators from microglial cells, and promoted M2 polarization in vitro, which in turn protected the co-cultured neurons from apoptosis via suppression of the NF-κB and MAPK signalling pathways. Furthermore, daily intraperitoneal injections of 80 mg/kg Rea into a rat model of SCI significantly improved the behavioural and histological indices, promoted M2 microglial polarization, alleviated neuronal apoptosis, and increased motor function recovery. Therefore, Rea is a promising therapeutic option for SCI and should be clinically explored.
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Apoptosis/efectos de los fármacos , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Microglía/efectos de los fármacos , Microglía/metabolismo , Neuronas/metabolismo , Fármacos Neuroprotectores/farmacología , Traumatismos de la Médula Espinal/metabolismo , Animales , Biomarcadores , Células Cultivadas , Modelos Animales de Enfermedad , Femenino , Mediadores de Inflamación/metabolismo , Microglía/inmunología , Modelos Biológicos , Actividad Motora , FN-kappa B/metabolismo , Extractos Vegetales/química , Extractos Vegetales/farmacología , Ratas , Rehmannia/química , Transducción de Señal , Traumatismos de la Médula Espinal/tratamiento farmacológico , Traumatismos de la Médula Espinal/etiología , Traumatismos de la Médula Espinal/rehabilitaciónRESUMEN
OBJECTIVE: This study was conducted to evaluate the acute and subchronic toxicity of gardenia yellow, a natural colorant widely used in China and other Asian countries. An acute toxicity test was performed in S-D rats of both genders and the lethal dose (LD50) of per oral gardenia yellow was estimated to be more than 15.0 g/kg·bw. In the subchronic study, gardenia yellow was orally administered to rats by gavage at doses of 0, 0.50, 1.50 and 4.50 g/kg·bw/day for 90 days followed by a recovery period of 28 days. No appreciable toxic-related changes were observed in the 0.50 g/kg·bw/day group. When the animals received gardenia yellow at 1.50 g/kg·bw/day or more, body weight loss was observed, and pigments began to deposit in several vital organs, resulting in significant changes of several hematological and biochemical indicators related to the nutritional status of the body, liver and kidney function, more severe in the high dose group. In the recovery period, the alterations of the clinical symptoms and parameters were relieved a lot. Based on the results of the current study, the no observed adverse effect level (NOAEL) of gardenia yellow E500 in rats was set to be 0.50 g/kg·bw/day.
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Gardenia/toxicidad , Extractos Vegetales/toxicidad , Administración Oral , Animales , Peso Corporal/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Femenino , Dosificación Letal Mediana , Hígado/efectos de los fármacos , Masculino , Nivel sin Efectos Adversos Observados , Ratas , Ratas Sprague-Dawley , Pruebas de Toxicidad Aguda , Pruebas de Toxicidad SubcrónicaRESUMEN
Contrast-induced acute kidney injury (CI-AKI) is a common complication of iodinated contrast medium administration during cardiac catheterization. Statin treatment has been shown to be associated with reduced risk of CI-AKI; however, the results are inconsistent, especially for patients with chronic kidney disease (CKD). Thus, we conducted a network meta-analysis to evaluate the effects of statins in the prevention of CI-AKI. We systematically searched several databases (including, Embase, PubMed, the Cochrane Library, and ClinicalTrials.gov ) from inception to January 31, 2018. The primary outcome was occurrence of CI-AKI in patients with CKD undergoing cardiac catheterization. Both pairwise and network meta-analysis were performed. Finally, 21 randomized controlled trials with a total of 6385 patients were included. Results showed that statin loading before contrast administration was associated with a significantly reduced risk of CI-AKI in patients with CKD undergoing cardiac catheterization (odds ratio: 0.46; P < .05). Atorvastatin and rosuvastatin administered at high dose may be the most effective treatments to reduce incidence of CI-AKI, with no difference between these 2 agents.
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Lesión Renal Aguda/inducido químicamente , Lesión Renal Aguda/prevención & control , Atorvastatina/administración & dosificación , Cateterismo Cardíaco/efectos adversos , Medios de Contraste/efectos adversos , Inhibidores de Hidroximetilglutaril-CoA Reductasas/administración & dosificación , Riñón/efectos de los fármacos , Insuficiencia Renal Crónica/epidemiología , Rosuvastatina Cálcica/administración & dosificación , Lesión Renal Aguda/epidemiología , Lesión Renal Aguda/fisiopatología , Anciano , Atorvastatina/efectos adversos , Investigación sobre la Eficacia Comparativa , Esquema de Medicación , Femenino , Humanos , Inhibidores de Hidroximetilglutaril-CoA Reductasas/efectos adversos , Riñón/fisiopatología , Masculino , Persona de Mediana Edad , Ensayos Clínicos Controlados Aleatorios como Asunto , Insuficiencia Renal Crónica/fisiopatología , Factores de Riesgo , Rosuvastatina Cálcica/efectos adversos , Resultado del TratamientoRESUMEN
Keshan disease is a potentially fatal cardiomyopathy in humans. Selenium deficiency, T-2 toxin, and myocarditis virus are thought to be the major factors contributing to Keshan disease. But the relationship among these three factors is poorly described. This study aims to explore whether selenium deficiency aggravates T-2 toxin-induced cardiomyocyte injury and its underlying mechanism. Cardiomyocytes were isolated from neonatal rat and cultured at the physiological (2.0⯵M) or lower concentrations of selenium with different concentrations of T-2 toxin. Our results showed that selenium deficiencies aggravated T-2 toxin-induced cardiomyocyte injury in a concentration-dependent manner as demonstrated by MTT bioassay, LDH activity, reactive oxygen species levels and caspase 3 protein expressions. T-2 toxin treatment significantly increased mRNA expressions for stress proteins GRP78 and CHOP in cardiomyocytes compared with the control. Selenium deficiencies further promoted GRP78, CHOP and p-eIF2α expressions. Knockdown of CHOP by the specific small interfering RNA eliminated the effect of selenium deficiencies on T-2 toxin-induced injury. It could be concluded that selenium deficiency aggravates T-2 toxin-induced cardiomyocyte injury through initiating more aggressive endoplasmic reticulum stress.
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Enfermedades Carenciales , Estrés del Retículo Endoplásmico/genética , Miocitos Cardíacos/patología , Selenio/deficiencia , Toxina T-2/toxicidad , Animales , Animales Recién Nacidos , Supervivencia Celular , Células Cultivadas , Chaperón BiP del Retículo Endoplásmico , Proteínas de Choque Térmico/genética , Ratas , Especies Reactivas de Oxígeno/metabolismo , Factor de Transcripción CHOP/genéticaRESUMEN
BACKGROUND: China has the world's largest tea plantation area in the world. To sustain high yields of the tea, multiple pesticides are used on tea crops to control pests. Organophosphorus (OP) pesticides are among the most widely used types of agricultural pesticides in China. As tea is a significant potential source of exposure to pesticide residues, the public concern has increased in relation to pesticide residues found in tea in China. The aim of the study was to estimate cumulative dietary exposure to OP residues from tea infusion for Chinese tea consumers to determine whether exposure to OP residues from tea infusion is a cause of health concern for tea consumers in China. METHODS: OP residue data were obtained from the China National Monitoring Program on Food Safety (2013-2014), encompassing 1687 tea samples from 12 provinces. Tea consumption data were obtained from the China National Nutrient and Health Survey (2002), comprising 506 tea consumers aged 15-82 years. The transfer rates of residues from tea leaves into tea infusions were obtained from the literature. The relative potency factor (RPF) approach was used to estimate acute cumulative exposure to 20 OP residues from tea infusion using methamidophos as the index compound. Dietary exposure was calculated in a probabilistic way. RESULTS: For tea consumers, the mean and the 99.9th percentile (P99.9) of cumulative dietary exposure to OP residues from tea infusion equalled 0.08 and 1.08 µg/kg bw/d. When compared with the acute reference dose (ARfD), 10 µg/kg bw/d for methamidophos, this accounts for 0.8 and 10.8% of the ARfD. CONCLUSIONS: Even when considering OP residues from vegetables, fruits and other foods, there are no health concerns based on acute dietary exposure to OP residues from tea infusion. However, it is necessary to strengthen the management of the OP pesticides used on tea in China to reduce the risk of chronic dietary exposure to OPs from tea infusion.
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Exposición Dietética/análisis , Compuestos Organofosforados/análisis , Residuos de Plaguicidas/análisis , Té/química , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , China , Femenino , Humanos , Masculino , Persona de Mediana Edad , Medición de Riesgo/métodos , Adulto JovenRESUMEN
The complete chloroplast sequence of Dendrobium officinale, an endangered and economically important traditional Chinese medicine, was reported and characterized. The genome size is 152,018 bp, with 37.5% GC content. A pair of inverted repeats (IRs) of 26,284 bp are separated by a large single-copy region (LSC, 84,944 bp) and a small single-copy region (SSC, 14,506 bp). The complete cp DNA contains 83 protein-coding genes, 39 tRNA genes and 8 rRNA genes. Fourteen genes contained one or two introns.
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Cloroplastos/genética , ADN de Cloroplastos/genética , Dendrobium/genética , Genoma del Cloroplasto/genética , Genoma de Planta/genética , Composición de Base/genética , Secuencia de Bases , China , Mapeo Cromosómico , Orden Génico/genética , Genes de Plantas/genética , Tamaño del Genoma/genética , Secuencias Invertidas Repetidas/genética , Medicina Tradicional China , Hojas de la Planta/genética , ARN Ribosómico/genética , ARN de Transferencia/genética , Análisis de Secuencia de ADNRESUMEN
Fungi have evolved powerful genomic and chemical defense systems to protect themselves against genetic destabilization and other organisms. However, the precise molecular basis involved in fungal defense remain largely unknown in Basidiomycetes. Here the complete genome sequence, as well as DNA methylation patterns and small RNA transcriptomes, was analyzed to provide a holistic overview of secondary metabolism and defense processes in the model medicinal fungus, Ganoderma sinense. We reported the 48.96 Mb genome sequence of G. sinense, consisting of 12 chromosomes and encoding 15,688 genes. More than thirty gene clusters involved in the biosynthesis of secondary metabolites, as well as a large array of genes responsible for their transport and regulation were highlighted. In addition, components of genome defense mechanisms, namely repeat-induced point mutation (RIP), DNA methylation and small RNA-mediated gene silencing, were revealed in G. sinense. Systematic bioinformatic investigation of the genome and methylome suggested that RIP and DNA methylation combinatorially maintain G. sinense genome stability by inactivating invasive genetic material and transposable elements. The elucidation of the G. sinense genome and epigenome provides an unparalleled opportunity to advance our understanding of secondary metabolism and fungal defense mechanisms.
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Ganoderma/genética , Genoma Fúngico , Mapeo Cromosómico , Cromosomas Fúngicos/química , Cromosomas Fúngicos/metabolismo , Metilación de ADN , Elementos Transponibles de ADN/genética , ADN de Hongos/química , ADN de Hongos/genética , ADN de Hongos/metabolismo , Ganoderma/clasificación , Silenciador del Gen , Familia de Multigenes , Filogenia , ARN Interferente Pequeño/metabolismo , Análisis de Secuencia de ADNRESUMEN
Twenty one-year-old local male dogs were randomly assigned into four groups (five dogs per group). The control and the ethylene glycol (EG) groups were fed basal diets without and with EG, and the EG+sodium selenite (EG+SS) and EG+selenium yeast (EG+SY) groups were fed basal diets with EG containing SS and SY, respectively. Blood, urine, and renal samples were taken after 18 weeks of feeding. The results showed that compared with the control group, the serum calcium levels and antioxidase activities significantly decreased in the EG group. Serum creatinine, urea nitrogen, and malondialdehyde (MDA) levels and urine calcium and oxalate levels significantly increased. Calcium oxalate crystal deposition and osteopontin (OPN) messenger RNA and protein expression in the renal tissues significantly increased. These changes above in the EG group were reversed within limits by adding selenium in the diets (both EG+SS and EG+SY groups). Further, compared with the EG+SS group, the EG+SY group showed better effects in decreasing the formation of EG-induced calcium oxalate renal calculi and OPN expression and improving antioxidant capability in dogs. It indicates that organic selenium has the potential value to alleviate the formation of EG-induced calcium oxalate renal calculi.
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Antioxidantes/metabolismo , Oxalato de Calcio/química , Glicol de Etileno/química , Cálculos Renales/prevención & control , Osteopontina/metabolismo , Selenio/administración & dosificación , Alimentación Animal , Animales , Nitrógeno de la Urea Sanguínea , Calcio/orina , Creatinina/sangre , Dieta , Perros , Masculino , Malondialdehído/sangre , Oxalatos/orina , Estrés Oxidativo , Selenio/química , UrinálisisRESUMEN
MAIN CONCLUSION: Twenty-nine genes related to phenolic acid biosynthesis were identified in the Salvia miltiorrhiza genome. Nineteen of these are described for the first time, with ten genes experimentally correlating to phenolic acid biosynthesis. Vast stores of secondary metabolites exist in plants, many of which possess biological activities related to human health. Phenolic acid derivatives are a class of valuable bioactive pharmaceuticals abundant in the widely used Chinese medicinal herb, Salvia miltiorrhiza. The biosynthetic pathway for phenolic acids differs in this species from that of other investigated plants. However, the molecular basis for this is unknown, with systematic analysis of the genes involved not yet performed. As the first step towards unraveling this complex biosynthetic pathway in S. miltiorrhiza, the current genome assembly was searched for putatively involved genes. Twenty-nine genes were revealed, 19 of which are described here for the first time. These include 15 genes predicted in the phenylpropanoid pathway; seven genes in the tyrosine-derived pathway; six genes encoding putative hydroxycinnamoyltransferases, and one CYP98A, namely CYP98A78. The promoter regions, gene structures and expression patterns of these genes were examined. Furthermore, conserved domains and phylogenetic relationships with homologous proteins in other species were revealed. Most of the key enzymes, including 4-coumarate: CoA ligase, 4-hydroxyphenylpyruvate reductase and hydroxycinnamoyltransferase, were found in multiple copies, each exhibiting different characteristics. Ten genes putatively involved in rosmarinic acid biosynthesis are also described. These findings provide a foundation for further analysis of this complex and diverse pathway, with potential to enhance the synthesis of water-soluble medicinal compounds in S. miltiorrhiza.
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Genes de Plantas , Hidroxibenzoatos/metabolismo , Salvia miltiorrhiza/genética , Secuencia de Aminoácidos , Cinamatos/metabolismo , Depsidos/metabolismo , Datos de Secuencia Molecular , Salvia miltiorrhiza/metabolismo , Ácido RosmarínicoRESUMEN
Porcine splenocytes were isolated in vitro, treated with different levels of dexamethasone (DEX), and stimulated by concanavalin A. Further, the normal (non-DEX-supplemented) or DEX-treated (0.01 µmol/L) splenocytes were incubated with 0, 0.5, 2, and 5 µmol/L Na2SeO3. The splenocyte proliferation, IL-2 production, intracellular glutathione peroxidase 1 (GPx1) mRNA level and activity and thioredoxin reductase 1 mRNA level were measured. The results showed that addition of 0.5 or 2 µmol/L Na2SeO3 significantly promoted normal and DEX-treated splenocyte proliferation, IL-2 production and GPx1 mRNA expression and activity (P < 0.05), respectively. The maximum effect was observed in DEX-treated splenocytes with 0.5 µmol/L Na2SeO3. Thus, our results show that the immune state modulation of Se is stronger in DEX-treated splenocytes than normal splenocytes. The mechanism underlying this effect may be increased in GPx1 expression induced by Se. Our results explain the controversy of varying reports on the immune state modulation induced by Se.
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Antiinflamatorios/farmacología , Dexametasona/farmacología , Inmunomodulación/efectos de los fármacos , Selenio/inmunología , Selenio/farmacología , Bazo/efectos de los fármacos , Porcinos/inmunología , Alimentación Animal/análisis , Animales , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Dieta/veterinaria , Suplementos Dietéticos/análisis , Interleucina-2/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Selenoproteínas/genética , Selenoproteínas/metabolismo , Porcinos/metabolismoRESUMEN
OBJECTIVE: To introduce the current foodborne illness report system in China. METHODS: Foodborne illness (food poisoning included) report system and food related unusual cases reported system were characterized by their report definitions, scopes and report procedures as well as their differences. RESULTS: From October, 2010 to June, 2012, there are 2961 centers of disease control and prevention and heath executive organizations at the different local levels registered in the foodborne illness (food poisoning included) report system and 1525 incidents were reported. There were 553 hospitals registered in the food related unusual cases reported system while only 38 cases reported. CONCLUSION: The foodborne illness report system has been set up in China and further efforts in capacities building are needed.
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Notificación de Enfermedades/estadística & datos numéricos , Enfermedades Transmitidas por los Alimentos/prevención & control , China , Notificación de Enfermedades/normas , Humanos , Programas Nacionales de SaludRESUMEN
Dendrobium officinale Kimura et Migo (Orchidaceae) is a traditional Chinese medicinal plant. The stem contains an alkaloid that is the primary bioactive component. However, the details of alkaloid biosynthesis have not been effectively explored because of the limited number of expressed sequence tags (ESTs) available in GenBank. In this study, we analyzed RNA isolated from the stem of D. officinale using a single half-run on the Roche 454 GS FLX Titanium platform to generate 553,084 ESTs with an average length of 417 bases. The ESTs were assembled into 36,407 unique putative transcripts. A total of 69.97% of the unique sequences were annotated, and a detailed view of alkaloid biosynthesis was obtained. Functional assignment based on Kyoto Encyclopedia of Genes and Genomes (KEGG) terms revealed 69 unique sequences representing 25 genes involved in alkaloid backbone biosynthesis. A series of qRT-PCR experiments confirmed that the expression levels of 5 key enzyme-encoding genes involved in alkaloid biosynthesis are greater in the leaves of D. officinale than in the stems. Cytochrome P450s, aminotransferases, methyltransferases, multidrug resistance protein (MDR) transporters and transcription factors were screened for possible involvement in alkaloid biosynthesis. Furthermore, a total of 1061 simple sequence repeat motifs (SSR) were detected from 36,407 unigenes. Dinucleotide repeats were the most abundant repeat type. Of these, 179 genes were associated with a metabolic pathway in KEGG. This study is the first to produce a large volume of transcriptome data from D. officinale. It extends the foundation to facilitate gene discovery in D. officinale and provides an important resource for the molecular genetic and functional genomic studies in this species.
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Dendrobium/genética , Proteínas de Plantas/genética , Transcriptoma , Alcaloides/biosíntesis , Vías Biosintéticas/genética , Dendrobium/enzimología , Marcadores Genéticos , Secuenciación de Nucleótidos de Alto Rendimiento , Medicina Tradicional China , Anotación de Secuencia Molecular , Proteínas de Plantas/metabolismo , Tallos de la Planta , Plantas Medicinales/enzimología , Plantas Medicinales/genética , Análisis de Secuencia de ADN , Repeticiones de TrinucleótidosRESUMEN
The study was carried out to evaluate the subchronic toxicity of meat derived from human lactoferrin gene-modified cattle in male and female Wistar rats. Rats were fed 5% or 10% transgenic meat diet, 5% or 10% conventional meat diet, or AIN93G diet for 90 days. During the study, body weight and food consumption were weighed weekly and clinical observations were conducted daily. At the end of the study, urinary examination, hematology and blood biochemistry examination, macroscopic and microscopic examinations were performed. There were no biologically significant differences in these factors between the rat groups fed transgenic meat diet and conventional meat diet. Therefore, the present 90-day rodent feeding study suggests that meat derived from the transgenic cattle is equivalent to meat from conventional cattle in use as dietary supplements.
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Bovinos/genética , Inocuidad de los Alimentos , Alimentos Modificados Genéticamente/toxicidad , Productos de la Carne/toxicidad , Alimentación Animal , Animales , Animales Modificados Genéticamente , Análisis Químico de la Sangre , Peso Corporal/efectos de los fármacos , Ingestión de Alimentos/efectos de los fármacos , Masculino , Tamaño de los Órganos/efectos de los fármacos , Ratas , Ratas Wistar , Pruebas de Toxicidad Subcrónica , UrinálisisRESUMEN
Salvia miltiorrhiza is an important medicinal plant with great economic and medicinal value. The complete chloroplast (cp) genome sequence of Salvia miltiorrhiza, the first sequenced member of the Lamiaceae family, is reported here. The genome is 151,328 bp in length and exhibits a typical quadripartite structure of the large (LSC, 82,695 bp) and small (SSC, 17,555 bp) single-copy regions, separated by a pair of inverted repeats (IRs, 25,539 bp). It contains 114 unique genes, including 80 protein-coding genes, 30 tRNAs and four rRNAs. The genome structure, gene order, GC content and codon usage are similar to the typical angiosperm cp genomes. Four forward, three inverted and seven tandem repeats were detected in the Salvia miltiorrhiza cp genome. Simple sequence repeat (SSR) analysis among the 30 asterid cp genomes revealed that most SSRs are AT-rich, which contribute to the overall AT richness of these cp genomes. Additionally, fewer SSRs are distributed in the protein-coding sequences compared to the non-coding regions, indicating an uneven distribution of SSRs within the cp genomes. Entire cp genome comparison of Salvia miltiorrhiza and three other Lamiales cp genomes showed a high degree of sequence similarity and a relatively high divergence of intergenic spacers. Sequence divergence analysis discovered the ten most divergent and ten most conserved genes as well as their length variation, which will be helpful for phylogenetic studies in asterids. Our analysis also supports that both regional and functional constraints affect gene sequence evolution. Further, phylogenetic analysis demonstrated a sister relationship between Salvia miltiorrhiza and Sesamum indicum. The complete cp genome sequence of Salvia miltiorrhiza reported in this paper will facilitate population, phylogenetic and cp genetic engineering studies of this medicinal plant.
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Genoma del Cloroplasto/genética , Plantas Medicinales/genética , Salvia miltiorrhiza/genética , Anticodón/genética , Secuencia de Bases , Mapeo Cromosómico , Codón/genética , Exones/genética , Genes de Plantas/genética , Variación Genética , Intrones/genética , Lamiaceae/genética , Repeticiones de Microsatélite/genética , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , Filogenia , Reproducibilidad de los ResultadosRESUMEN
OBJECTIVE: To identify Ephedrae Herba using the ITS2 barcode and to secure its quality and safety in medication. METHOD: Total genomic DNA was isolated from Ephedrae Herba and its closely related species. Nuclear DNA ITS2 sequences were amplified, and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. The Kimura 2-Parameter (K2P) distances were calculated using software MEGA 5.0. Identification analyses were performed using BLAST1, Nearest Distance and neighbor-joining (NJ) methods. RESULT: The intra-specific genetic distances of Ephedrae Herba were ranged from 0 to 0.002. The inter-specific genetic distances between Ephedrae Herba and its closely related species were ranged from 0.004 to 0.034. All the three methods showed that ITS2 could discriminate Ephedrae Herba from its closely related species correctly. CONCLUSION: The ITS2 region is suitable to be used for authentication of Ephedrae Herba, and our study further confirmed the effectiveness of ITS2 to identify traditional Chinese medicinal materials.
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Código de Barras del ADN Taxonómico/métodos , ADN Espaciador Ribosómico/genética , Ephedra sinica/genética , Ephedra sinica/clasificaciónRESUMEN
OBJECTIVE: To identify Menthae Haplocalycis Herba and its closely related species using DNA barcoding technique. METHOD: Total genomic DNA was isolated from Mentha canadensis and its closely related species. Nuclear DNA ITS2 sequences were amplified, and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner V3.0. The Kimura 2-Parameter (K2P) distances were calculated using software MEGA 5.0. Identification analyses were performed using BLAST1, Nearest Distance and neighbor-joining (NJ) methods. RESULT: The intra-specific genetic distances of M. canadensis were ranged from 0 to 0.006, which were lower than inter-specific genetic distances between M. canadensis and its closely related species (0.071-0.231). All the three methods showed that ITS2 could discriminate M. canadensis from its closely related species correctly. CONCLUSION: The ITS2 region is an efficient barcode for identification of Menthae Haplocalycis Herba, which provides a scientific basis for fast and accurate identification of the herb.
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Código de Barras del ADN Taxonómico/métodos , ADN Espaciador Ribosómico/genética , Plantas Medicinales/genética , Plantas Medicinales/clasificación , Análisis de Secuencia de ADN/métodosRESUMEN
OBJECTIVE: To investigate the effect of recombinant human lactoferrin (rhLf) separated and purified from milk of transgenic cows on improving the iron status of rats suffered from iron deficiency anemia (IDA). METHOD: The IDA model of female weaning Wistar rats was induced by iron deficient diet, and the rats were then randomly divided into 6 groups (12 rats in each group, based on their body weight and the concentration of hemoglobin) : group A (the model control ), group B, C and D (rhLf 0.375. 0.75 or 2.25 g/kg BW), group E (ferrous lactate 0.011 g/kg BW), and group F (ferrous lactate 0.011 g/kg BW + rhLf 0.375 g/kg BW). Test substances were administered by gavage daily for 8 weeks. Related indexes, including general health condition, body weight, hematological parameters, free erythrocyte protoporphyrin (FEP), serum iron (SI), total iron binding capacity (TIBC), serum ferritin (SF), serum Cu and serum Zn, were determined. RESULTS: After 8 weeks of supplementation, body weight, RBC, Hb, SI, TS and SF levels of rats in group E and F were improved and significantly higher than those in group A, while levels of FEP, TIBC and Zn were lower. Indexes of rats in group D were also significantly different from those in group A but with a less degree than those in group E or F. Compared with group A, there were no obvious difference in iron status for group B or C except for more RBC and lower levels of FEP and Zn in group C, as well as the lower level of Zn in group B. SI, TS and SF levels in group F were significantly higher but TIBC was lower than those in group E. CONCLUSION: The iron status of IDA rats could be improved to some degree by the supplementation of rhLf alone, but the effect was weaker than that of ferrous lactate. The effect of combining rhLf with ferrous lactate was more intensive than ferrous lactate or rhLf administered individually.
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Anemia Ferropénica/tratamiento farmacológico , Hierro/sangre , Lactoferrina/uso terapéutico , Anemia Ferropénica/sangre , Animales , Animales Recién Nacidos , Femenino , Humanos , Lactoferrina/biosíntesis , Lactoferrina/genética , Ratas , Ratas Wistar , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/genética , Proteínas Recombinantes/uso terapéuticoRESUMEN
OBJECTIVE: To investigate the effect of high doses apigenin on antioxidase activity of and DNA oxidative damages in rats. METHODS: 100 Wistar rats were randomly divided into five groups including control, dose one to four groups. The trial lasted 13 weeks, the blood samples and liver of rats were collected at the end of the experiment. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) and levels of malondialdehyde (MDA) were determinde, 8-OHdG content, which is indicative of DNA damage were assessed. RESULTS: At 2 g/kg and 4 g/kg apigenin, the activity of SOD and GSH-Px in male rat liver significantly decreased, at 2 g/kg bw dose, the activity of male rat liver GST also greatly decreased. At the dose of 4 g/kg apigenin, GSH-Px activity in serum of both sex rats significantly decreased, its male rat GST activity at dose of 8 g/kg apigenin also greatly decreased. CONCLUSION: High dose apigenin could decrease antioxidase activity of male rat, but, it didn't cause DNA damage.