A comprehensive quality evaluation strategy of Mailuoning oral liquid based on fingerprint, qualitative and quantitative analyses.

In order to obtain comprehensive quality evaluation of one traditional Chinese patent medicine of Mailuoning oral liquid (MLN), one smart strategy combined by fingerprint, qualitative and quantitative analyses were carried out in this study. Firstly, the fingerprints of MLN were established by HPLC-UV and HPLC-ELSD, and explained the similarity of twenty-seven batches of MLN by similarity analysis (SA). Secondly, qualitative analysis was performed by high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (HPLC-QTOF-MS/MS). A total of 60 compounds were identified or tentatively identified based on chemical standards and fragmentation information. Finally, the quantitative method based on UPLC combined with triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS) was developed for the simultaneous determination of 40 target compounds. The results showed that MLN samples of different productive year were clearly discriminated and eight compounds (5-hydroxymethyl-2-furaldehyde, neochlorogenic acid, loganic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isoacteoside, angoroside C) were selected as differential markers for MLN. In a word, this strategy including fingerprint, identification of chemical composition and multiple-component quantification could be well applied to modern quality evaluation of MLN, which could be valuable for the further quality control of more other traditional Chinese patent medicines.

Targeted characterization and guided isolation of chemical components in Scrophulariae Radix based on LC-MS.

How to achieve rapid characterization and efficient isolation of chemical components from traditional Chinese medicines (TCMs) is what the researchers have been exploring. Herein, a strategy integrated diagnostic ion filtering (DIF) and selected ion recording (SIR)-based screen was firstly proposed and successfully applied for targeted characterization and guided isolation of the chemical components from Scrophulariae Radix, one of TCMs. After acquiring the Q-TOF-MS/MS untargeted data, 128 compounds were characterized based on DIF, a self-built database and comparison of the related literatures, in which 38 compounds were reported for the first time. Subsequently, the SIR method of UPLC-QqQ-MS/MS was adopted to guide the isolation of potential new compounds. Finally, three new compounds together with one known compound with the same skeleton were isolated, and unambiguously elucidated by NMR and acid hydrolysis. These results indicated that this integrated analytical approach is effective and reliable in targeted characterizing chemical components and isolating new compounds from the extract of Scrophulariae Radix.

Xingnaojing ameliorates synaptic plasticity and memory deficits in an Aß1-42 induced mouse model of Alzheimer's disease.

The accumulation of insoluble amyloid ß (Aß) peptides is one of the pathological changes in Alzheimer's disease (AD), which induced synaptic plasticity impairment and excitatory amino acid toxicity associated with decreased memory function. Xingnaojing (XNJ), a well-known prescription in traditional Chinese medicine, has been used for the treatment of stroke for many years in China. In this study, we aim to investigate the therapeutic effects of XNJ in a hippocampus of Aß1-42 induced mouse model of AD which showed significant memory loss and impaired synaptic morphology and function. Treatment of XNJ could attenuate spatial and working memory dysfunction, increase dendritic spine density and improve long-term potential (LTP) induction. In addition, XNJ treatment significantly increased the level of N-methyl-d-aspartate receptors (NMDARs) and inhibit the NMDA/α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) ratio in AD mice. XNJ treatment also activated the AKT/mechanistic target of rapamycin (mTOR) pathway, while inhibition of the mTOR pathway by rapamycin could reverse the protective effects of XNJ treatment. In conclusion, XNJ protected against synaptic plasticity and memory impairment in AD mice via the activation of AKT/mTOR signaling pathway, suggesting XNJ as an alternative treatment for AD.