Asperosaponin VI protects alcohol-induced hepatic steatosis and injury via regulating lipid metabolism and ER stress.

BACKGROUND: Asperosaponin VI (AVI) is a natural triterpenoid saponin isolated from Dipsacus asper Wall with documented anti-inflammatory and bone protective effects. Our previous work reported that AVI protects the liver of septic mice from acute inflammatory damage. In this paper, we further explored the protective effect and the potential mechanisms of AVI in alcoholic fatty liver disease (AFLD). METHODS: The Lieber-Decarli model was constructed to evaluate the effect of AVI on AFLD in C57BL/6 J mice. Additional in vitro work was performed to investigate HepG2 cells exposed to alcohol, then analyzed the degree of liver injury by detecting the ALT and AST levels both in the liver and serum. H&E staining and Sirius red staining were used to evaluate the histopathology variations in the liver. Further, observe lipid droplets in the cytoplasm by Oil Red O staining. We detected the expression of inflammatory cytokines with qualitative PCR; ROS, MDA, SOD, and GSH-px levels were analyzed to observe oxidative stress. Finally, exploring the activation of AMPK signaling pathway by real-time PCR and Western blotting. RESULTS: Histological examination of liver tissue combined with serum ALT and AST levels showed a significant protective effect of AVI against alcoholic liver injury in AFLD mice. Compared with the model group, AVI evidently improved antioxidant capacity, reduced inflammatory response and lipid accumulation both in vitro and in vivo. For mechanically, it was found that AVI up-regulated phosphorylation level of AMP-activated protein kinase (AMPK) and inhibited the endoplasmic reticulum stress (ER) pathway in AFLD. CONCLUSION: AVI protects mice from alcohol-induced hepatic steatosis and liver injury through activating AMPK signaling and repress ER stress, suggesting that it might be a potential therapeutic agent for AFLD.

[Chemical constituents from Pulsatillae Radix].

Chemical constituents of ethanol extract of Pulsatillae Radix were investigated. The n-butanol fraction of ethanol extract of Pulsatillae Radix was isolated and purified by macroporous resin and silica gel column chromatography and semi-preparative high performance liquid chromatography. The triterpenoid glycosides were identified by multiple spectral methods. Six compounds were obtained from the n-butanol fraction of ethanol extract of Pulsatillae Radix and identified as 23-aldehyde-cussosaponin C(1), cussosaponin C(2), anemoside B4(3), akebia saponin D(4), pulchinenoside E3(5), and hederacoside C(6). Among them, compound 1 was a new compound.

Study on the effect of Cr(VI) removal by stimulating indigenous microorganisms using molasses.

Molasses have a prominent effect on the bioremediation of Cr(VI) contaminated groundwater. However, its reaction mechanism is not detailed. In this paper, the removal of Cr(VI) with different carbon sources was compared to explore the effect and mechanism of the molasses. The addition of molasses can completely remove 25 mg/L Cr(VI), while the removal efficiency by glucose or emulsified vegetable oil was only 20%. Molasses could rapidly stimulate the reduction of Cr(VI) by indigenous microorganisms and weakened the toxicity on bacteria. The average removal rate of Cr(VI) was 0.42 mg/L·h, 10 times that of glucose system. Compared with glucose, molasses can remediate Cr(VI) at a higher concentration (50 mg/L), and the carbohydrate acted as microbial nutrients. Direct and indirect reduction acted together, the Fe(II) content in the aquifer medium increased from 1.7% to 4.7%. The addition of molasses extract into glucose system could increased the removal rate of Cr(VI) by 2-3 times, and the ions of molasses had no significant effect on the reduction. Excitation emission matrix fluorescence spectra and electrochemical analysis proved that the molasses contained humic acid-like substances, which had the ability of electron shuttle and improved the reduction rate of Cr(VI). In the process of bioreduction, the composition of molasses changed and the electron transport capacity increased from 104.2 to 446.5 µmol/(g C), but these substances could not be used as electron transport media to continuously enhance the reduction effect. This study is of great significance to fully understand the role and application of molasses.