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1.
Menopause ; 28(12): 1340-1350, 2021 10 04.
Artículo en Inglés | MEDLINE | ID: mdl-34610616

RESUMEN

OBJECTIVE: Postmenopausal women are at increased risk of metabolic diseases such as obesity and diabetes. Therefore, the chemoprevention of postmenopausal changes in health via dietary supplements is important. Syringic acid (SA) is a phenolic compound present in the fruit of the assai palm, Euterpe oleracea, and in the mycelium of the shiitake mushroom, Lentinula edodes. This compound shows no affinity for estrogen receptors and may exert disease-preventive effects. Reportedly, dietary SA ameliorates high-fat diet-induced obesity in mice; however, its effects on estrogen deficiency-induced obesity are still unclear. Therefore, in this study, we investigated whether and how dietary SA affects these factors in ovariectomized (OVX) mice. METHODS: Ten-week-old OVX mice were fed SA-containing diets (100 mg/kg body weight/d) for 12 weeks. Their body weights, food intake, and uterus weights as well as other parameters were measured and comparisons were made with mice in the control group. RESULTS: Dietary SA did not affect the body weight, food intake, or uterus weight of OVX mice over the study period; however, the SA-fed group showed lower fat mass (ie, visceral, subcutaneous, and total fat) than the OVX-control group (11.1 ±â€Š3.3 vs. 8.3 ±â€Š2.4, P < 0.05; 7.9 ±â€Š1.1 vs. 5.9 ±â€Š1.6, P < 0.05; 19.0 ±â€Š4.2 vs. 14.1 ±â€Š3.8, P < 0.05, respectively). Furthermore, blood analysis revealed that SA-treatment resulted in a dose-dependent decrease and increase in serum triglyceride (59.2 ±â€Š8.3 vs. 43.9 ±â€Š12.2 mg/dL P < 0.05) and adiponectin (7.7 ±â€Š0.3 vs. 9.5 ±â€Š0.6 µg/mL, P < 0.05) levels, respectively. CONCLUSIONS: These results suggest that the SA diet improves lipid metabolism without affecting the uterus in OVX mice. Therefore, dietary SA has potential applicability for the prevention of postmenopausal obesity and type 2 diabetes.


Asunto(s)
Diabetes Mellitus Tipo 2 , Animales , Peso Corporal , Dieta Alta en Grasa/efectos adversos , Femenino , Ácido Gálico/análogos & derivados , Humanos , Ratones , Obesidad/prevención & control , Ovariectomía
2.
Biosci Biotechnol Biochem ; 82(8): 1408-1416, 2018 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-29629624

RESUMEN

Cutaneous exposure to food allergens can predispose individuals to food allergies. Soybean, a major allergenic food, is an ingredient in various cosmetic products. However, the types of soybean proteins that are percutaneously sensitizing in humans or animal models remain unknown. In this study, BALB/c mice were dorsally shaved and epicutaneously exposed to a crude soybean extract including sodium dodecyl sulfate or distilled water alone. Specific IgEs secreted in response to 7S globulin (Gly m 5), 11S globulin (Gly m 6), Gly m 3, and Gly m 4 were measured using enzyme-linked immunosorbent assays or immunoblots. Exposure to soybean extract elicited the secretion of soybean-specific IgEs. Of the soybean proteins, 7S and 11S globulins acted as percutaneous sensitizers in 6/9 mice (67%). Additionally, IgE bound specifically and preferentially to the 7S globulin ß subunit. In conclusion, this is the first report to identify percutaneously sensitizing soybean allergens in a mouse model.


Asunto(s)
Alérgenos/administración & dosificación , Hipersensibilidad a los Alimentos , Globulinas/administración & dosificación , Glycine max/química , Extractos Vegetales/farmacología , Piel/efectos de los fármacos , Administración Cutánea , Alérgenos/efectos adversos , Animales , Western Blotting , Ensayo de Inmunoadsorción Enzimática , Globulinas/efectos adversos , Humanos , Inmunoglobulina E/metabolismo , Ratones Endogámicos BALB C , Extractos Vegetales/administración & dosificación , Piel/inmunología , Glycine max/inmunología
3.
Biosci Biotechnol Biochem ; 82(7): 1207-1215, 2018 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-29606082

RESUMEN

The levels of food allergens in worm-wounded or non-wounded green soybeans (edamame) and mature soybeans were investigated by immunoblotting and enzyme-linked immunosorbent assay (ELISA), using allergen-specific antibodies. Non-wounded and worm-wounded soybeans showed similar total protein profiles after Coomassie brilliant blue staining, but some protein bands were observed to have been changed by worm wounding. Immunoblotting with specific antibodies for major soybean allergens (Gly m 5, Gly m 6, Gly m Bd 30 K, and Kunitz soybean trypsin inhibitor) revealed that protein band profiles and intensities were not significantly changed by worm wounding. In contrast, levels of the pollen-related soybean allergens Gly m 4 and Gly m 3 were strongly increased by worm wounding in both green and mature soybeans, as detected by immunoblotting and ELISA. These results suggested that the pollen-related food allergen risk (i.e., oral allergy syndrome; OAS) from soybeans might be enhanced by worm wounding of soybeans.


Asunto(s)
Alérgenos/metabolismo , Hipersensibilidad a los Alimentos/inmunología , Glycine max/metabolismo , Lepidópteros/fisiología , Polen/inmunología , Alérgenos/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Western Blotting , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Conducta Alimentaria , Proteínas de Plantas/metabolismo , Colorantes de Rosanilina , Glycine max/parasitología
4.
J Nutr Sci Vitaminol (Tokyo) ; 64(6): 483-486, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30606971

RESUMEN

Oral tolerance prevents allergic responses, but cutaneous exposure to food allergens predisposes individuals to food allergies. Soybean, a major allergenic food, is also an ingredient in various cosmetic products. However, it remains to be determined whether oral tolerance prevents percutaneous sensitization to soybean proteins in humans or animal models. In this study, BALB/c mice were divided into three groups; the SS group fed a soybean-containing diet, and the CS and control (C) groups fed a soybean-free diet. After being dorsally shaved, the CS and SS groups were epicutaneously exposed to a soybean extract while the control group was exposed to only the carrier. Specific IgE and IgG1 immunoglobulins secreted in response to the soybean proteins were measured using enzyme-linked immunosorbent assays. Exposure to the soybean extract elicited the secretion of IgE and IgG1 specific for Gly m 5 and Gly m 6, and trypsin inhibitor. Oral soybean consumption attenuated the secretion of all the soybean-specific IgEs and IgG1s, suggesting that percutaneous sensitization to soybean proteins is attenuated by oral tolerance.


Asunto(s)
Antígenos de Plantas/uso terapéutico , Dieta , Glycine max/química , Hipersensibilidad/prevención & control , Enfermedades de la Piel/prevención & control , Piel/efectos de los fármacos , Proteínas de Soja/uso terapéutico , Administración Cutánea , Alérgenos/administración & dosificación , Alérgenos/uso terapéutico , Animales , Antígenos de Plantas/administración & dosificación , Antígenos de Plantas/efectos adversos , Cosméticos/química , Ensayo de Inmunoadsorción Enzimática , Femenino , Hipersensibilidad a los Alimentos/inmunología , Intolerancia Alimentaria , Hipersensibilidad/metabolismo , Inmunoglobulina E/metabolismo , Inmunoglobulina G/metabolismo , Ratones Endogámicos BALB C , Enfermedades de la Piel/inducido químicamente , Enfermedades de la Piel/inmunología , Enfermedades de la Piel/metabolismo , Proteínas de Soja/administración & dosificación , Proteínas de Soja/efectos adversos , Proteínas de Soja/inmunología , Glycine max/efectos adversos , Glycine max/inmunología
5.
Biosci Biotechnol Biochem ; 77(4): 789-95, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23563566

RESUMEN

Royal jelly (RJ), the exclusive food for queen bees, is taken as a dietary supplement because it is highly rich in nutrients. However, RJ is known to induce an anaphylactic response in some individuals. We evaluated in the present study the hypoallergenicity of alkaline protease-treated RJ in vitro and in vivo. We first confirmed that this treated RJ contained the same levels of vitamins, minerals and specific fatty acid as in untreated RJ. We then showed that the IgE-binding capacity of the treated RJ was very significantly reduced by conducting in vitro assays of the blood from RJ-sensitive patients. An in vivo skin-prick test on the RJ-sensitive patients also showed that, in the majority of the patients (3 out of 4 tested), the treated RJ did not evoke any allergenic response. It is thus advantageous to prepare hypoallergenic RJ by a protease enzyme treatment for its safe consumption.


Asunto(s)
Alérgenos/inmunología , Alérgenos/metabolismo , Proteínas Bacterianas/metabolismo , Abejas/química , Endopeptidasas/metabolismo , Ácidos Grasos/inmunología , Ácidos Grasos/metabolismo , Adulto , Alérgenos/química , Animales , Basófilos/inmunología , Basófilos/metabolismo , Ácidos Grasos/química , Ácidos Grasos Monoinsaturados/análisis , Femenino , Liberación de Histamina/inmunología , Humanos , Hidrólisis , Mastocitos/inmunología , Mastocitos/metabolismo , Minerales/análisis , Proteolisis , Piel/inmunología , Vitaminas/análisis
6.
J Nutr Sci Vitaminol (Tokyo) ; 59(5): 462-9, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24418881

RESUMEN

Miso paste (miso), a fermented soybean food, is popular in Japan and other Asian countries. However, the soybean is known to induce an allergenic response in some individuals. In the present study, we evaluated the allergenicity of various kinds of miso available in Japan. Total proteins were extracted from Amakuti-kome miso, Karakuti-kome miso, Mugi-miso and Mame-miso, and the protein profiles were analyzed. The major protein bands detected in the intact soybean extract were not present in any of the miso samples, which instead showed various low molecular weight protein bands of approximately 10-25 kDa. The existence levels of six major soybean allergens were determined by Western blotting using specific antibodies. We found that the allergen levels varied among miso and allergen types; however, allergen levels were consistently lower in miso than in the soybean extract. We obtained similar results for IgE-ELISA experiments using serum IgE from soybean allergy patients. Taken together, these results indicate that compared to soybean extract, various types of miso contain small quantities of intact soybean allergens. Additionally, several lines of evidence indicated that the allergen levels were exceptionally low in the dark-colored Karakuti-kome miso and Mame-miso, which are produced with relatively long fermentation periods, suggesting that the duration of fermentation might be a key factor in the hypoallergenicity of miso.


Asunto(s)
Alérgenos/análisis , Antígenos de Plantas/análisis , Dieta , Hipersensibilidad a los Alimentos/inmunología , Fragmentos de Péptidos/efectos adversos , Alimentos de Soja/efectos adversos , Proteínas de Soja/efectos adversos , Alérgenos/efectos adversos , Alérgenos/química , Alérgenos/metabolismo , Antígenos de Plantas/efectos adversos , Antígenos de Plantas/química , Antígenos de Plantas/metabolismo , Aspergillus/metabolismo , Bacillus/metabolismo , Western Blotting , Dieta/etnología , Ensayo de Inmunoadsorción Enzimática , Fermentación , Hipersensibilidad a los Alimentos/sangre , Hipersensibilidad a los Alimentos/dietoterapia , Hipersensibilidad a los Alimentos/etiología , Humanos , Inmunoglobulina E/análisis , Inmunoglobulina E/metabolismo , Japón , Peso Molecular , Fragmentos de Péptidos/análisis , Fragmentos de Péptidos/aislamiento & purificación , Fragmentos de Péptidos/metabolismo , Pigmentación , Extractos Vegetales/efectos adversos , Extractos Vegetales/química , Proteolisis , Alimentos de Soja/análisis , Alimentos de Soja/economía , Alimentos de Soja/microbiología , Proteínas de Soja/análisis , Proteínas de Soja/química , Proteínas de Soja/metabolismo , Factores de Tiempo
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