RESUMEN
Three new neolignan derivatives (1-3), together with three known isolariciresinol derivatives (4-6) were isolated from Selaginella picta. Their structures were elucidated by spectroscopic methods (1D/2D NMR, HRESIMS and CD). All isolated compounds were assayed on the neuroprotective activity against the injury of HT-22 cells induced by L-Glutamate in vitro. All compounds displayed potent protective effect on HT-22 cells.
Asunto(s)
Lignanos/química , Fármacos Neuroprotectores/química , Selaginellaceae/química , Animales , Evaluación Preclínica de Medicamentos , Ácido Glutámico/toxicidad , Lignanos/farmacología , Espectroscopía de Resonancia Magnética , Ratones , Estructura Molecular , Fármacos Neuroprotectores/farmacología , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
Five new abietane diterpenoids (complanatins A-E, 1-5) have been isolated from the club moss Lycopodium complanatum, along with two known abietane diterpenoids (xanthoperol and sugiol). Their structures were determined by comprehensive analysis of 1D, 2D NMR, CD and HRESIMS data. The cytotoxic effects of five compounds (1-4, 7) were evaluated in three human lung cancer cell lines (MSTO-211H, NCI-H2052 and NCI-H226). Compounds 3 and 4 exhibited cytotoxic activities against the three cell lines. In addition, a plausible biogenetic pathway of compounds 1-7 was proposed.
Asunto(s)
Abietanos/aislamiento & purificación , Antineoplásicos Fitogénicos/aislamiento & purificación , Lycopodium/química , Abietanos/farmacología , Antineoplásicos Fitogénicos/farmacología , Vías Biosintéticas , Línea Celular Tumoral , Humanos , Estructura MolecularRESUMEN
Three new compounds, pictalignans A (1), B (2), C (3), along with three known analogues, syringaresinol (4), 3,3',5-trimethoxy-4',7-epoxy-8,5'-neoligan-4',9,9'-triol (5), 4,9-dihydroxy-4',7-epoxy-8',9'-dinor-8,5'-neolignan-7'-oic acid (6) were isolated from the 75% aqueous ethanol extract of Selaginella picta. Their structures were established by physicochemical properties and spectroscopic methods, and absolute configurations of new compounds were elucidated by experimental and calculated ECD spectra. Compounds 1-3 are neolignans with additional one or two C6-C3 structural units attached to hydroxypropyl group, which are extremely rare in nature. All new compounds exhibited moderate protective effect against the injury of HT-22 cells induced by L-Glutamate in vitro, and compound 1 showed better protective effect than positive drug with the concentrations of 10⯵M to 15⯵M.