A new triterpenoid tetrasaccharide from the root of Scrophularia ningpoensis / 药学学报

A new oleanane-type triterpenoid glycoside, 3beta-O-(f-D-xylopyranosyl-(1-->2)-[beta-D-glucopyranosyl (1-->4)-beta-D-glucopyranosyl-(1 -->3)]-beta-D-fucopyranosyl-11, 13(18) diene-olean-23alpha, 28 diol (1), along with three known phenylethyl glycosides (2-4), was isolated from the root of traditional Chinese medicine Scrophularia ningpoensis. Among them, compounds 2 and 3 were obtained from Scrophularia genus for the first time. The structure of compound 1 was elucidated on the basis of spectroscopic method including 1D, 2D NMR and HR-ESI-MS.

Phenolic components from Petasites tricholobus / 中国中药杂志

<p><b>OBJECTIVE</b>To study the anti-inflammatory and antioxidant effects of Petasites tricholobus and its phenolic components.</p><p><b>METHOD</b>Phenolic compounds were separated purified by column chromatographic methods such as macroporous resin D-101, silica gel, ODS, MCI GEL CHP 20P, Sephadex LH-20. Their structures were identified on the basis of physicochemical property and multiple spectral data.</p><p><b>RESULT</b>Nineteen phenolic compounds were separated from 95% ethanol extracts from P. tricholobus Franch. and identified as sulfonated benzyl glucoside (1), 3-(4beta-D-glucopyranosyloxy-3, 5-dimethoxy) -phenyl-2E-propenol (2), dihydrosyringin (3), tangshenosides II (4), 4-hydroxy-2,6-dimethoxyphenol-1-O-beta-D-glucopyranoside (5), 4-hydroxymethyl-2, 6-dimethoxyphenyl-1-O-beta-D-glucopyranoside (6), arbutin (7), rutin (8), kaempferol-3-O-alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-glucopyranoside (9), quercetin-3-O-beta-D-glucopyranoside (10), kaempferol-3-O-beta-D-glucopyranoside (11), afzelin (12), petasiphenol (13), caffeic acid (14), chlorogenic acid (15), 2-hydroxy-5-acetylbenzoic acid (16), p-hydroxy-benzoic acid (17), protocatechuic aldehyde (18) , and p-hydroxy-phenylpropionic acid (19).</p><p><b>CONCLUSION</b>Above result shows that phenolic compounds contained in P. tricholobus mainly include simple phenols, phenolic glycosides, coffee acid and flavonoid glycosides. Among them, compound 1 was separated from the composite family for the first time; compounds 2-7, 9, 11, 12, 16, 19 were separated from the genus Petasites for the first time, and the others were separated from the plant for the first time. These compounds have been proved to have pharmacological effects such as anti-inflammation, antibiosis, antioxidantion.</p>

Identification of Rhizoma Acori Talarinowii and Determination of Its β-asarone Content / 中药新药与临床药理

Objective To establish a method for identification and quality control of Rhizoma Acori Talarinowii. Methods The medicinal herb of Rhizoma Aeori Talarinowii was identified by its origin and TLC. High-performance liquid chromatography (HPLC) was used to determine the content of β-asarone in Rhizoma Acori Talarinowii. Results The results of TLC showed that there were some spots with the same color in the position having the same relative retention time between the tested medici-hal material and the control. The results of HPLC showed that a good iinearity was in the range of 0. 1～0. 6 βg, the regression equation was Y=37158. 2X-589. 5(r=0. 999 7), and the average recovery was 96. 78 % (RSD=0. 95 %). Conclusion The method is simple and reproducible, and can be used for the quality control of Rhizoma Acori Talarinowii.

Identification of Rhizoma Acori Talarinowii and Determination of Its ?-asarone Content / 中药新药与临床药理

Objective To establish a method for identification and quality control of Rhizoma Acori Talarinowii. Methods The medicinal herb of Rhizoma Acori Talarinowii was identified by its origin and TLC. High-performance liquid chromatography(HPLC) was used to determine the content of ? -asarone in Rhizoma Acori Talarinowii. Results The results of TLC showed that there were some spots with the same color in the position having the same relative retention time between the tested medicinal material and the control. The results of HPLC showed that a good linearity was in the range of 0.1～ 0.6 ? g, the regression equation was Y=37158.2X-589.5(r=0.999 7), and the average recovery was 96.78 % (RSD = 0.95 % ). Conclusion The method is simple and reproducible, and can be used for the quality control of Rhizoma Acori Talarinowii.