Clinical treatment of lumbar disc herniation.

OBJECTIVE: To investigate the clinical effect of radiofrequency ozone and injection of anti-inflammatory analgesic solution into the internal orifice of nerve root combined with traditional Chinese medicine hook operation in the treatment of lumbar disc herniation. METHODS: Patients with lumbar disc herniation who were admitted to our hospital on December 20, 2017 and June 19, 2019 were selected as the main research objects, and the included patients were divided into control group, basic group and comprehensive group by random number table method. Control group was treated with radiofrequency ozone therapy, basic group was treated with injection of anti-inflammatory analgesic solution into the internal orifice of nerve root in addition to the control group, comprehensive group was treated with traditional Chinese medicine hook operation in addition to the basic group. The clinical treatment effects were observed. RESULTS: A total of 153 patients were included in this study, including 40 in the control group, 40 in the basic group, and 73 in the comprehensive group. The results showed that the NRS scores of control group were 3±0.98, 2±0.93 and 2±0.85 at 1 month, 3 months and 1 year after treatment, respectively. NRS scores in the basic group were 3±0.18, 2±0.33, and 2±0.15, respectively. NRS scores in the comprehensive group were 2±0.78, 1±0.54, and 1±0.77, respectively. Compared with the control group, there were significant differences in basic group and comprehensive group at each time point (P < 0. 05). At the same time, compared with the basic group, the NRS score of the comprehensive group was statistically different (P < 0.05). CONCLUSION: Radiofrequency ozone and injection of anti-inflammatory analgesic solution into the internal orifice of nerve root combined with hook operation can obtain good short-term and medium-term effects in the treatment of lumbar disc herniation. It is a safe and effective minimally invasive treatment method. KEY WORDS: Internal orifice of nerve root, Lumbar disc herniation, Ozone.

[Protective effects and machanism of muskone on pheochromocytoma cell injure induced by glutamate].

OBJECTIVE: To explore the mechanism of glutamate (Glu)-induced PC12 cell apoptosis and the protective effect of muscone. METHOD: PC12 cells were randomly divided into six groups: normal group (Normal), model group injured by glutamate (Glu), nimodipine group (Nim) and muskone groups (Mus) of high, middle and low doses. The PC12 cells were pretreated with or without different concentrations of muskone for 30 min and then exposed to glutamate. MTT assay for cell survival, flow cytometric detection of apoptotic cells, DCF assay for reactive oxygen species (ROS) and flow cytometric assay was performed to determine the mitochondrial membrane potential in PC12 cell. RESULT: PC12 cell damage, the concentrations of [Ca2+], and apoptosis induced by Glu were decreased after being administrated with paeoniflorin. CONCLUSION: Muskone inhibited Glu-induced apoptosis in PC12 cells. The mechanism is related to inhibiting intracellular Ca2+ overload and maintaining mitochondral membrance potential.