Analysis of the Levels of Vitamin A, D and E in Peripheral Blood of Children in Zhejiang Province.

BACKGROUND: This study aims to investigate the serum fat-soluble vitamins A, D, and E levels of children in Zhejiang Province, and to provide a reference range of fat-soluble vitamins for children in Zhejiang Province. METHODS: Between May 2019 and December 2019, 871 children who sent peripheral blood samples to Hangzhou Biozon Medical Institute Co., Ltd. for fat-soluble vitamin A, D, and E analysis were selected, including 432 boys and 439 girls. After the peripheral blood was collected, the serum A, D, and E levels were measured, and the differences in age, gender, season, and region were compared. RESULTS: With age, the level of vitamin A gradually increased (p < 0.01), and vitamin D2, D3, and total vitamin D all increased first and then decreased. Vitamin A (131.79 ± 47.05 ng/mL vs. 121.96 ± 41.01 ng/mL) and E (5.87 ± 2.23 µg/mL vs. 5.56 ± 2.13 µg/mL) levels of girls were higher than boys (p < 0.01), and vitamin D3 (15.25 ± 6.16 ng/mL vs.15.17 ± 7.26 ng/mL) and total vitamin D (18.09 ± 7.01 ng/mL vs. 17.03 ± 8.79 ng/mL) levels of boys were higher than girls (p < 0.01). From the perspective of regional distribution, the levels of vitamin A, D2, and E in Ningbo were higher than those in Hangzhou and other regions. The seasonal distribution of vitamin A and E levels were highest in summer, while vitamin D3 and D levels were highest in fall. The average vitamin concentrations were as follows: vitamin A was (126.81 ± 44.42) ng/mL; vitamin D2 was (1.84 ± 3.16) ng/mL, vitamin D3 was (15.71 ± 6.75) ng/mL, total vitamin D was (17.55 ± 7.91) ng/mL, and vitamin E was (5.72 ± 2.19) µg/mL. The reference value ranges of vitamin A, D2, D3, D, and E were (52.44 - 222.27) ng/mL, (0.01 - 11.66) ng/mL, (4.92 - 30.96) ng/mL, (4.92 - 30.96) ng/mL, and (2.66 - 10.92) µg/mL, respectively. CONCLUSIONS: The childrens' fat soluble vitamin levels in Zhejiang province show significant differences in age, gender, season, and regional distribution. Corresponding reference standards should be formulated as soon as possible, and vitamin supplements should be targeted and reasonable to ensure the healthy development of children.

Effects of Catheter Tracking Management on Urinary Tract Function and Infection Rates in Patients With Spinal Cord Injury.

Context: Patients with bone-marrow injuries, such as spinal cord injuries (SCIs), usually have urinary dysfunction, changes to the urethra's anatomical structure, and pathophysiological changes of the urinary system, which can lead to urodynamic changes. If a patient receives improper treatment, repeated infections of the urinary system can easily occur, causing hydronephrosis and damage to renal function. Objective: The study intended to explore the effects of catheter follow-up management for patients with SCIs on the function of the bladder and the urinary tract and on urinary tract infections (UTIs), selecting antibiotics reasonably according to a bacterial culture and drug sensitivity test. Design: The research team designed a randomized controlled trial. Setting: The study took place at the Hebei Cangzhou Hospital of Integrated Traditional Chinese Medicine (TCM)-Western Medicine (WM) in Cangzhou City, Hebei Province, People's Republic of China. Participants: Participants were 92 patients with SCIs who were treated at the hospital between January 2020 and December 2021. Intervention: The research team randomly divided participants into an intervention group (n = 45) and a control group (n = 47). The control group received routine treatment, while the intervention group received catheter follow-up management. Outcome Measures: At baseline and postintervention after six weeks of treatment, the research team: (1) examined participants' bladder function, (2) examine urodynamic indexes including measurement of the maximum bladder volume, maximum urethral closure pressure, maximum urinary flow rate, and maximum detrusor pressure, and (3) assessed participants' QoL using the World Health Organization Quality of Life Questionnaire Abbreviated (WHOQOL-BREF). Results: Improvements in bladder function, urodynamic indexes, QoL, and UTIs occurred in both groups. The intervention group's: (1) total effective rate for bladder function was 91.11%, which was significantly higher than that of the control group (P = .022); (2) maximal bladder volume, urethral closure pressure, and urinary flow rate were 365.59 ± 54.43 ml, 81.19 ± 8.8 cmH2O, and 18.60 ± 2. 43 ml/s, respectively, and were significantly higher than those of the control group (all P = .000); (3) maximal detrusor pressure was 47.48 ± 5.64 cmH2O, which was significantly lower than that of the control group (p=0.000); (4) scores on the WHOQOL-BREF's subdimensions and total score were significantly higher than those in the control group: psychological, 17.92 ± 1.55; physiological, 30.30 ± 1.82; independence, 22.43 ± 1.40; social relations, 16.82 ± 1.32; environment, 21.19 ± 1.85; and total score, 110.02 ±16.64 (all P = .000); (5) incidence of urinary tract infection was 17.78 which was significantly lower than that of the control group (P = .003). The distribution of bacterial species in the UTIs of the intervention and control groups wasn't significantly different (P = .869). The two bacterial groups were Escherichia coli and Enterococcus. Drug sensitivity tests showed that the Escherichia coli were less susceptible to gentamicin, levofloxacin, and piperacillin than to ciprofloxacin, and the Enterococcus were less susceptible to gentamicin, ciprofloxacin, and levofloxacin than to piperacillin. Conclusions: For patients with SCIs, catheter follow-up management can be helpful in restoring the function of the bladder and urinary tract, can improve patients' QoL, and reduce their rate of UTIs. Clinically, medical practitioners should select antibiotics reasonably according to a bacterial culture and drug-sensitivity test.

Difference in intestinal microbiome between children with atopic dermatitis and healthy children / 中华皮肤科杂志

Objective To investigate the difference in intestinal microbiome between children with atopic dermatitis(AD)and healthy children. Methods Totally, 35 children with AD were enrolled from the Department of Dermatology, Jiading Hospital of Traditional Chinese Medicine from April 2015 to April 2017, and 27 healthy children served as control group. Total DNA was extracted from the feces of the subjects, and the V3-V4 region of the 16S rRNA gene of the bacteria was amplified by PCR. High-throughput sequencing was performed using the Illumina Miseq sequencing platform to analyze the diversity of bacterial flora. The top 15 abundant bacteria were determined at phylum, genus, and species levels, and compared between the two groups. Statistical analysis was carried out using Wilcoxon rank sum test. Results The intestinal microbiome in the two groups mainly consisted of Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria. At the phylum level, the abundance of Bacteroidetes and Fusobacteria was significantly lower in the AD group(29.16％± 19.96％, 0.06％± 0.17％, respectively)than in the healthy control group(39.06％± 15.98％, 0.50％± 1.71％, respectively, P=0.042, 0.041). At the genus level, the abundance of Bacteroides was significantly lower in the AD group(23.77％± 18.08％)than in the healthy control group(33.1％± 15.75％, P=0.029). There was no significant difference in the distribution of the top 15 abundant species between the two groups(all P>0.05). Conclusion There are some differences in the composition of intestinal microbiome and relative abundance of bacteria between children with AD and healthy children.

Difference in intestinal microbiome between children with atopic dermatitis and healthy children / 中华皮肤科杂志

Objective@#To investigate the difference in intestinal microbiome between children with atopic dermatitis (AD) and healthy children.@*Methods@#Totally, 35 children with AD were enrolled from the Department of Dermatology, Jiading Hospital of Traditional Chinese Medicine from April 2015 to April 2017, and 27 healthy children served as control group. Total DNA was extracted from the feces of the subjects, and the V3-V4 region of the 16S rRNA gene of the bacteria was amplified by PCR. High-throughput sequencing was performed using the Illumina Miseq sequencing platform to analyze the diversity of bacterial flora. The top 15 abundant bacteria were determined at phylum, genus, and species levels, and compared between the two groups. Statistical analysis was carried out using Wilcoxon rank sum test.@*Results@#The intestinal microbiome in the two groups mainly consisted of Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria. At the phylum level, the abundance of Bacteroidetes and Fusobacteria was significantly lower in the AD group (29.16% ± 19.96%, 0.06% ± 0.17%, respectively) than in the healthy control group (39.06% ± 15.98%, 0.50% ± 1.71%, respectively, P = 0.042, 0.041) . At the genus level, the abundance of Bacteroides was significantly lower in the AD group (23.77% ± 18.08%) than in the healthy control group (33.1% ± 15.75%, P = 0.029) . There was no significant difference in the distribution of the top 15 abundant species between the two groups (all P > 0.05) .@*Conclusion@#There are some differences in the composition of intestinal microbiome and relative abundance of bacteria between children with AD and healthy children.

Clinical efficacies of Jiangzhi Decoction for lipid metabolism disorder patients with traditional Chinese medicine syndrome of phlegm and turbidity retardation / 中华全科医师杂志

A total of 101 lipid metabolism disorder patients with traditional Chinese medicine (TCM) syndrome of phlegm and turbidity retardation were collected and randomly divided into group A:Jiangzhi Decoction (JZF) alone (n =30),group B:atorvastatin (ATV) alone (n =39) and group C:JZF plus ATV (n =32).Lipid level,liver and kidney function,total effective rate and TCM scores were observed after a course of 2 weeks.Total effective rate:Group C was significantly superior to group B (90.62％ vs.76.92％,u =1.97,P ＜ 0.05) ; TCM score:Group C had a statistically superior advantage over groups A andB (C vs.A,90.62％ vs.80.00％,u=1.98,P＜0.05; Cvs.B,90.62％ vs.51.28％,u=4.11,P ＜0.01); TC and Improvements of total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C):Group C demonstrated marked decreases in both TC and LDL (CTC & CLDL-C vs.group A,P ＜ 0.01 ; CTC & CLDL-C vs.group B,P ＜ 0.01),no abnormal liver or kidney function occurred during the follow-up period.Thus JZF and ATV may have synergic lipid-lowering effects.And single uses of JZF and ATV have no marked differences in short term efficacy.

Study on clinical efficacy and endothelial function protection of Tianma Gouteng Granule in interfering primary hypertension patients with hyperactivity of liver-yang / 中国医师杂志

Objective To investigate clinical anti-hypertension effect,clinical traditional Chinese medicine (TCM) syndrome improvement and endothelium function such as cardio-ankle vascular index (CAVI),flow-mediated dilatation (FMD) and endothelial micro-particle (EMP) protection of Tianma Gouteng Granule in interfering primary hypertension patients with hyperactivity of liver-yang.Methods Sixty six primary hypertension patients with hyperactivity of liver-yang were randomly divided into the control group (standardized treatment,n =35) and the treatment group (Tianma Gouteng Granule + standardized treatment,n =31) according to the random number method.In four weeks' follow-up treatment,the changes in clinical efficiency,TCM syndrome improvement,CAVI,FMD and EMP were observed.Results (1)Total clinical efficiency ratio was 71.43％ in control vs.70.97％ in treatment without statistical significance (P ＞0.05).(2) TCM syndrome improvement was better in treatment group than that in control group (93.55％ vs 74.29％,x2 =4.39,P ＜ 0.05),especially in improving symptoms such as dissiness,headache,dysphoria in chest,palms and soles,insomia and tinnitus (P ＜ 0.05).(3) In the aspect of endothelium protection,treatment group can greatly improve CAVI compared with control (L-CAVI ∶ t =2.11,P ＜0.05;R-CAVI∶ t =2.15,P ＜0.05) ; the levels of FMD(t =-4.65,P ＜0.01) and EMP (t =4.35,P ＜ 0.01)were significantly improved after treatment (P ＜ 0.05) while no statistical meaning was found between two group.Conclusions Tianma Gouteng Granule can improve TCM syndrome,decrease CAVI level.FMD and EMP were significantly decreased in treatment group.Multi-factor regression analysis showed that EMP level was related to pulse pressure.