RESUMEN
The in vivo gene delivery of E. coli cytosine deaminase (cd) cDNA and systemic 5-fluorocytosine (5-FC) administration have been studied extensively because of their clinical relevance to cancer gene therapy. This approach has the potent advantage of a stronger bystander effect compared to the previous thymidine kinase suicide gene system of the herpes simplex virus. However, 5-fluorouracil (5-FU), an active metabolite in cd with 5-FC therapy, is not always effective for every type of tumor since the enzymes responsible for further drug metabolism vary significantly in each tissue. In this study, we aimed to increase the sensitivity of 5-FU by transduction of thymidine phosphorylase (dThdPase) cDNA into brain tumor cells. After retroviral transfer of the cDNA, we obtained 9L murine gliosarcoma cells showing stable expression of the target enzyme (9L-dThdPase). The growth of the cells was identical to wild type (9L-WT) or control-vector transfected (9L-Neo) cells in vitro. Sensitivity to 5-FU was increased in 9L-dThdPase cells. After the adenoviral delivery of cytosine deaminase gene into these cells, 9L-dThdPase cells also demonstrated an increased sensitivity to 5-FC. Moreover, we showed that transduction of dThdPase cDNA prolongs the survival of animals bearing intracerebral tumors after experimental in vivo cytosine deaminase gene therapy. These results suggest that transduction of thymidine phosphorylase may be a beneficial approach to increasing the efficacy of cd/5-FC suicide gene therapy in certain types of tumor.
Asunto(s)
Antimetabolitos Antineoplásicos/farmacología , Neoplasias Encefálicas/terapia , ADN Complementario/genética , Flucitosina/farmacología , Terapia Genética/métodos , Gliosarcoma/terapia , Nucleósido Desaminasas/genética , Timidina Fosforilasa/genética , Adenoviridae/genética , Animales , Antimetabolitos Antineoplásicos/farmacocinética , Neoplasias Encefálicas/enzimología , Neoplasias Encefálicas/genética , Citosina Desaminasa , ADN Complementario/administración & dosificación , Flucitosina/farmacocinética , Fluorouracilo/farmacocinética , Fluorouracilo/farmacología , Vectores Genéticos/genética , Gliosarcoma/enzimología , Gliosarcoma/genética , Masculino , Nucleósido Desaminasas/metabolismo , Ratas , Ratas Endogámicas F344 , Timidina Quinasa/genética , Timidina Quinasa/metabolismo , Timidina Fosforilasa/biosíntesis , Timidina Fosforilasa/metabolismo , Transducción GenéticaRESUMEN
We evaluated the effectiveness of transtracheal heating and humidification system in maintaining body temperature during general anesthesia with low flow gases in 12 gastric cancer patients. Patients were divided into two group; Control group A in which a hot-water circulating system was used and group B in which a transtracheal heating and humidification system by ANAMED HUMITUBE was used, during gastric cancer operation. Compared to the hot-water circulating system, the transtracheal heating and humidification system is more effective for maintaining body temperature and humidification after abdominal lavage by warm saline water. But there was no difference between the two groups about awakening from general anesthesia. We concluded that transtracheal heating and humidification system by ANAMED HUMITUBE is effective in maintaining body temperature under general anesthesia with low flow gases.
Asunto(s)
Anestesia General/instrumentación , Temperatura Corporal , Hipertermia Inducida/instrumentación , Tráquea , Anciano , Humanos , Persona de Mediana Edad , Monitoreo Intraoperatorio , Neoplasias Gástricas/cirugía , Tráquea/fisiologíaRESUMEN
Five new resin-glycosides, named merremosides a (1), b (2), c (3), d (4), and e (5), were isolated from the tuber of Merremia mammosa (LOUR.) HALLIER f. (Convolvulaceae), an Indonesian medicinal plant. The structures of 1, 2, 3, 4, and 5 have been elucidated on the basis of chemical and physiochemical evidence, including syntheses of (11S)-(+)- and (11R)-(--)-jalapinolic acid (16b and 18b) and the glycosidic acid designated as merremoside i (6).
Asunto(s)
Glicósidos/química , Oligosacáridos/química , Plantas Medicinales/química , Secuencia de Carbohidratos , Glicósidos/aislamiento & purificación , Datos de Secuencia Molecular , Estructura Molecular , Oligosacáridos/aislamiento & purificación , Resinas de Plantas/químicaRESUMEN
Four new resin-glycosides named merremosides f (6), g (7), h1 (8), and h2 (9) were isolated from the tuber of Merremia mammosa (Lour.) Hallier f. (Convolvulaceae), an Indonesian medicinal plant. Their chemical structures have been elucidated on the bases of their chemical and physicochemical properties.
Asunto(s)
Glicósidos/química , Oligosacáridos/química , Plantas Medicinales/química , Secuencia de Carbohidratos , Glicósidos/aislamiento & purificación , Datos de Secuencia Molecular , Estructura Molecular , Oligosacáridos/aislamiento & purificación , Resinas de Plantas/químicaRESUMEN
Bioassay-guided fractionation monitored by mushroom tyrosinase (EC 1.14.18.1) activity, afforded six inhibitors from three Bolivian medicinal plants, Buddleia coriacea, Gnaphalium cheiranthifolium, and Scheelea princeps. These inhibitors, which are all known phenolic compounds, inhibited the oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) mediated by a mushroom tyrosinase.