Fingerprints of different samples of processed Rhizoma Coptidi by HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To set up the HPLC fingerprints of different samples of processed Rhizoma Coptidi (RC) in order to offer evidence for identifying different processed RC and preliminary discussion on processing mechanism.</p><p><b>METHOD</b>Seven different samples of processed RC were determined by HPLC and the results were analyzed by Hierarchical clustering and similarity evaluation system for chromatographic fingerprint of TCM.</p><p><b>RESULT</b>The characteristic of common peaks for fingerprints of different samples of processed RC is distinct, but there are differences among various processed RC. The different samples of processed RC were classified into two and the result of hierarchical clustering is correlated with the property of traditional Chinese drug.</p><p><b>CONCLUSION</b>It is proved that the method which is convenient, rapid and repeat well can be used to identify different samples of processed RC. The change of property of different processed RC is related with processing adjuvant, the possible mechanism is that new components are produced or the contents of known components have changed due to the adjuvant during processing.</p>

Probing into objectivity of adverse drug reaction of traditional Chinese medicine / 中国中药杂志

The adverse drug reaction (ADR) of traditional Chinese medicine (TCM) has been concerned nowadays. Some bans were published to limit their use abroad. There wasn't a feasible criterion to reply to the ADR of TCM yet in China. The ADR of 6 kinds of typical TCM which state attached importance to was chosen to discuss. Based on the retrospect investigation, the objectivity of ADR was explored in this study.

Thermodynamic study on antibacterial effect of different extracts from Radix Isatis / 中国结合医学杂志

<p><b>OBJECTIVE</b>To study and analyze the antibacterial effects of different extracts from Radix Isatis.</p><p><b>METHODS</b>Staphylococcus aureus was used as the studied object in the experiment. Antibacterial effects of extracts from Radix Isatis were observed by thermocalrimetry on Staphylococcus aureus, together with common pharmacological experiments.</p><p><b>RESULTS</b>The total extract, ethyl acetate (EtOAc) extract, n-butylalcohol (nBuOH) extract, chloroform (CHCl(3)) extract and petroleum (P.E.) extract had antiviral effects to some extent while the residue after extracting had no antibacterial activity. The potency of antiviral activity among them was as follows: nBuOH extract > EtOAc extract > CHCl(3) extract > total extract > P.E. extract.</p><p><b>CONCLUSION</b>The antibacteriall effects of Radix Isatis were not limited to any active portion, showing that Radix Isatis exerts its antibacterial effects by cooperation of different active fractions in varied ways.</p>

Effects of different extracts from Radix isatidis on lymphocytes of mice by biothermodynamics / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the different extracts from Radix Isatidis on multiplication of mice lymphocytes.</p><p><b>METHOD</b>Lymphocytes were separated and cultured. Immunological activities of different extracts from Radix Isatidis were studied by thermodynamics and the results were tested by the conventional pharmacological experiments.</p><p><b>RESULT</b>The results showed that the water extract and residue had significant immunological effects while organic solvent extracts had immunological activity to some extent.</p><p><b>CONCLUSION</b>The comparison of immunological activity among the extracts from Radix Isatidis were as follows: residue after extracting > general extract > nBuOH extract > EtOAc extract > CHCl3 extract > P E extract.</p>