Effects of black cohosh on the plasminogen activator system in vascular smooth muscle cells.

OBJECTIVE: The rhizome of the Cimicifuga racemosa plant (commonly known as black cohosh) has been used for menopausal complaints. Studies regarding the cardiovascular effects of black cohosh are lacking. We investigated the effect of black cohosh on the plasminogen activator system in cultured vascular smooth muscle cells (VSMCs). METHODS: VSMCs were isolated from rat aortae. Expression of plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) proteins were evaluated by Western blot analysis and enzyme-linked immunosorbent assay, respectively. The activities of PAI-1 and t-PA in the conditioned media were assessed by fibrin overlay zymography. A 40% 2-propanol extract of black cohosh was used. RESULTS: Black cohosh extract (BcEx) stimulated the protein expression of PAI-1, but it did not affect that of t-PA. Vitamin E, a potent antioxidant, inhibited the BcEx-induced increase in PAI-1 expression, while ICI 182,780, an estrogen receptor antagonist, had no effect. Fibrin overlay zymography revealed that BcEx increased the activity of PAI-1 in the conditioned media, while concurrently decreasing that of free t-PA by inducing a binding to PAI-1. CONCLUSIONS: BcEx induces PAI-1 protein expression in the VSMCs likely via an oxidant mechanism. It also stimulates the enzyme activity of PAI-1 and reduces that of free t-PA. These findings suggest that black cohosh might exert a negative influence on fibrinolysis.

Reproductive health characteristics of urban South Korean women.

AIMS: To characterize the reproductive health of urban South Korean women of reproductive age. METHODS: A cross-sectional online survey on menstrual patterns, sexual behavior, utilization of contraception, and perception of gynecological diseases was administered to 500 women from 15 to 39 years of age in five major cities of South Korea. RESULTS: Among the respondents, 76.8 and 25% experienced dysmenorrhea and abnormal uterine bleeding, respectively. Two-thirds of the respondents with dysmenorrhea used analgesics. After analgesics, herbal medicine was the next most commonly used treatment. The mean age of first sexual intercourse was 24.3 years, and 68.8% of the sexually active women used contraception. The most commonly used contraceptive method was the condom (79.7%), and many women (up to 62.2%) demonstrated a distorted understanding of oral contraceptives. Although many women were aware of general gynecological diseases such as cervical cancer (77%), endometriosis (64.4%), and leiomyoma (62.6%), only 22.2% of the respondents had visited a clinic for a gynecological examination, and among those who had visited a clinic, the main reason for the visit was pregnancy (44.1%). CONCLUSION: The results of this survey provide insight into and allow us to characterize the reproductive health status of urban South Korean women.

Modulation of the genomic estrogen receptor pathway by water extracts of Cirsium japonicum.

We investigated the estrogenic activity of Cirsium japonicum water extracts, which have long been used to treat vascular-related diseases. The activity of the extracts was characterized in a transient transfection system, using estrogen receptor isoforms and estrogen-responsive luciferase plasmids in HEK 293 cells. The extract activated both and estrogen receptors. Activation was inhibited by the estrogen receptor antagonist ICI 182,780, indicating that the effects were mediated through the estrogen receptor isoforms. Treatment with the extracts increased expression of the progesterone receptor and pS2 genes and expression of estrogen receptor was decreased in MCF-7 cells. These results suggested that the Cirsium japonicum water extracts showed estrogenic effects and may be a potential clinical application for treatment of estrogen related vascular diseases.