The mechanisms of biogenic methane metabolism by synergistic biodegradation of coal and corn straw.

The mechanisms associated with the biomethane metabolism through the synergistic biodegradation of both coal and corn straw were explored to improve the utilization rate of corn straw. This applies to the filling of the goaf with corn straw and the production of biomethane using indigenous bacteria in the mine water with coal. The results showed that new macromolecular substances (e.g., Tetracosane and Pentacosane) were produced on the third day. A lower coal rank leads to a lower biodegradation rate of low-molecular-weight substances (e.g., butyric acid and valeric acid). Under the addition of coal samples, the biodegradation rate of cellulose, hemicellulose and lignin in corn straw could reached up to 29.82%, 35.79% and 6.16%, respectively. The addition of corn straw promoted the complementary advantages of archaeal genera (such as Methanosarina and Methanospirillum) and decreased the adverse bacterial genera (such as Desulfovibrio and Pseudomonas) in the fermentation system of single coal.

Supplementation of Sesamin Alleviates Stress-Induced Behavioral and Psychological Disorders via Reshaping the Gut Microbiota Structure.

Sesamin, a lignan from sesame seed, has been reported to attenuate chronic mild stress-induced depressive-like behaviors. Gut microbiota play pivotal roles in mediating psychological behaviors by regulating gut barrier integrity and systemic inflammatory responses. Here, we found that oral sesamin administration (50 mg/kg·bodyweight/day) significantly attenuated depressive, aversive, repetitive, and anxiety-like behaviors in a long-term multiple nonsocial stress-treated mice model. Sesamin inhibited stress-induced gut barrier integrity damage, reduced circulating lipopolysaccharide (LPS) levels, and suppressed neuroinflammatory responses. Moreover, sesamin treatment also restructured the gut microbiome by enhancing the relative abundances of Bacteroidales and S24-7. The correlation analysis indicated that the microbiota composition changes were strongly correlated with behavioral disorders, serotonin, norepinephrine, and LPS levels. In conclusion, sesamin has preventive effects on stress-induced behavioral and psychological disorders, which might be highly related to the reshaped microbiota composition. This study provides a clue for understanding the systemic mechanism of anti-depression effects of sesamin.

Doliroside A from Dolichos falcata Klein suppressing amyloid ß-protein 42 fibrillogenesis: An insight at molecular level.

A bioactive chemical constituent, doliroside A, from Chinese traditional herbal medicine Dolichos falcata Klein was isolated, purified and identified by 60% ethanol extraction, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy. Molecular interaction mechanism between doliroside and amyloid ß42 protein was evaluated by thioflavin T fluorescence (ThT), circular dichroism (CD), atomic force microscope (AFM), and differential scanning calorimeter (DSC) from the aspects of kinetics, secondary structure, morphology, and thermodynamics, respectively. Results show that the purity of doliroside A is 99.9% by HPLC, and its chemical structure is identified by 1H- and 13C-NMR. Doliroside A is observed to be concentration-dependent inhibiting the fibrillation of Aß42 with the IC50 value of 26.57 ± 1.6 µM. CD and DSC results imply that doliroside A can bind to the nuclei and oligomers of Aß42 to form a stable complex and suppress Aß42 fibrillation. AFM images show that doliroside A, after bound to the nuclei and oligomers, redirect Aß42 into off-pathway, amorphous oligomers. These findings not only provide a full insight into the molecular interaction mechanisms between Aß42 and doliroside A, but also facilitate the development of new native anti-AD drug of doliroside A compound.