[Review of chemical constituents, pharmacological effects and clinical applications of Jiaotai Pills and predictive analysis of its quality marker(Q-marker)].

Jiaotai Pills is a traditional medical prescription to treat the incompatibility of heart and kidney. It has the distinctive functions of heart and kidney communication, sedation and hypnosis, anti-anxiety and depression, as well as the improvement of insulin resistance. However, this pill is broadly used to cure insomnia, anxiety, depression, and diabetes in the contemporary clinical trials. Based on the article, it illustrates the research progress of the chemical ingredients, pharmacological actions, and clinical applications of Jiaotai Pills. With respect to the "five principles" of Q-marker in Chinese medicine, the Q-marker of Jiaotai Pills is comprehensively predicted and analyzed, noting that berberine, epiberberine, coptisine chloride, palmatine chloride, berberine chloride, berberrubine chloride, ferulic acid, cinnamic acid, cinnamaldehyde, proanthocyanidin B2 can be treated as the Q-marker of Jiaotai Pills. In addition, these components of Q-marker have been selected as indicators to provide a significant reference for the quality control and surveillance research of Jiaotai Pills.

[Establishment of rat heart-kidney insomnia model consistent with traditional Chinese medicine syndrome and its serum metabolomics].

Enzyme-linked immunosorbent assay(ELISA) and metabolomics were used to analyze and compare two animal models of heart-kidney insomnia, in order to explore a more ideal animal model and preliminarily explore the essence of heart-kidney insomnia. Based on the clinical symptoms and disease characteristics of heart-kidney insomnia, the animal model of heart-kidney insomnia was reproduced through intraperitoneal injection with p-chlorophenylalanine(PCPA) and multi-factor interaction. The animal model of disease-syndrome combination was evaluated by behavioral observation, ELISA and metabolomics. Wistar rats were randomly divided into normal group, PCPA group and compound model group(FH). The rats' behavior, body weight, adrenal index and spleen index were recorded. The levels of corticotropin releasing hormone(CRH) and adrenocorticotropin(ACTH) in serum were detected by ELISA, and the differential metabolites in serum were detected by UPLC-QE-MS. The body weight and adrenal index in FH group were significantly lower than those in PCPA group(P<0.05); whereas ACTH and CRH in FH group were significantly higher than those in PCPA group by ELISA; nine potential biomarkers were identified by serum sample statistics. There were four main metabolic pathways in cardiorenal insomnia: pentose phosphate metabolism, alanine, aspartic acid and glutamic acid metabolism, histidine metabolism, and taurine and subtaurine metabolism. PCPA and multi-factor interaction method can successfully replicate the insomnia model, but multi-factor modeling method is more similar to clinical traditional Chinese medicine syndrome. Animal behavior, ELISA and metabolomics were used to evaluate the rat model of cardiorenal insomnia from in vitro to in vivo, from macro to micro, and from individual to the whole.

[Quality Evaluation of Rhei Radix et Rhizoma Based on SPE and HPLC Characteristic Chromatogram with An Internal Standard].

Objective: To establish a quality evaluation method for Rhei Radix et Rhizoma based on SPE and internal standard HPLC characteristic chromatogram. Methods: Using water reflux extraction and SPE methods, the total Rhei Radix et Rhizoma anthraquinones were obtained. By HPLC method, the characteristic chromatogram of Rhei Radix et Rhizoma was established,and the qualitative research was performed with DAD and MSn. Relative area of the characteristic peaks in the samples was calculated by the addition of an internal standard to evaluate the quality of Rhei Radix et Rhizoma. Results: The results showed that the optimum condition were as follows,the solid-liquid ratio was 1∶ 150; the extraction time was 60 min. SPE optimum condition for the introduction of Accu BOND Ⅱ SPE ODS-C18 Cartridges SPE were as follows, the loaded concentration was 1 m L, the SPE column was rinsed twice with 3 m L 0. 3% formic acid, then rinsed with 3 m L 25% methanol( containing 0. 3% formic acid) and finally eluted with 3 m L of methanol. The recovery rate was 96. 3% ~ 103%. With 5-hydroxymethylfurfural as an internal standard, the characteristic chromatogram was established, and twelve peaks were selected as characteristic peaks. In ten batches of samples, the RSD of relative retention time was < 3%,and the relative area of the characteristic peaks were calculated. The principal component analysis and cluster analysis with the results of the Chinese Pharmacopoeia( 2015 edition),which indicated that the characteristic chromatogram could reflect the anthraquinones in Rhei Radix et Rhizoma, and distinguish the Rheum palmatum, Rheum tanguticum and Rheum officinale. Conclusion: It is concluded that the established method is simple,fast and specific, which can provide a reference for the evaluation of the quality of Rhei Radix et Rhizoma.

[Optimization of Scutellaria baicalensis extraction process by orthogonal experiment combined with pharmacodynamic index].

To optimize the Scutellaria baicalensis extraction process, the filter paper method and the bacteriostatic ratio method were adopted to determine the in vitro bacteriostatic efficacy of water extracts and 60% alcohol extracts from S. baicalensis. The quantitative analysis of multi-components by single-marker (QAMS) was used to determined the contents of four active components, baicalin, wogonoside, baicalein and wogonin. In addition, with the bacteriostatic ratio and the overall desirability of the contents of four active components as indexes, the orthogonal experiment was adopted to detect the effect of water addition, extraction frequency and extraction time. The optimal extraction process was to add 12 times of water for the first time, 10 times of water for the second time, extract for 2 time, 2 h for each time. This optimization process is stable and feasible, with a higher bacteriostatic ratio in extracts.