RESUMEN
Soil microplastics (MPs) have attracted widespread attention recently. Most studies have explored how soil MPs affect the soil's physicochemical parameters, matter circulation, and soil microbial community assembly. Similarly, a key concern in agricultural development has been the use of phosphorus (P) fertiliser, which is essential for plant health and development. However, the relationship between MPs and phosphate fertilisers and their effects on the soil environment and plant growth remains elusive. This study assessed the influence of adding low-density polyethylene MPs (1%) with different phosphate fertiliser application rates on microbial communities and rice biomass. Our results showed that MPs changed the structure of soil bacterial and phoD-harbouring microbial communities in the treatment with P fertiliser at the same level and suppressed the interactions of phoD-harbouring microorganisms. In addition, we found that MPs contamination inhibited rice growth; however, the inclusion of P fertiliser in MP-contaminated soils reduced the inhibitory action of MPs on rice growth, probably because the presence with P fertiliser promoted the uptake of NO3--N by rice in MP-contaminated soils. Our results provide further insights into guiding agricultural production, improving agricultural management, and rationally applying phosphate fertilisers in the context of widespread MPs pollution and global P resource constraints.
Asunto(s)
Oryza , Suelo , Suelo/química , Microplásticos , Plásticos , Fertilizantes/análisis , Oryza/microbiología , Fósforo , FosfatosRESUMEN
Several studies demonstrate that estradiol can prevent arterial calcification. However, little is known regarding the effect of puerarin, a phytoestrogen extracted from Radix Puerariae, on arterial calcification. The aim of the present study was to determine whether puerarin reduced osteoblastic differentiation of calcifying vascular smooth muscle cells (CVSMCs). The CVSMCs were isolated from mice aorta and treated with different concentrations of puerarin. The alkaline phosphatase (ALP) activity, osteocalcin secretion and Runx2 expression were determined. To examine whether estrogen receptors (ERs) PI3K and Akt play a role in this effect, ICI182789, phosphoinositide 3-kinase (PI3K) inhibitor, LY294002, or the Akt inhibitor, 1L-6-hydroxymethyl-chiro-inositol 2-(R)-2-O-methyl-3-O-octadecylcarbonate (HIMO) was used. Our results showed puerarin could inhibit ALP activity, osteocalcin secretion and Runx2 expression in CVSMCs. Puerarin could induce the activation of Akt. Furthermore, pretreatment of ICI182780, LY294002, HIMO could abolish the effect of puerarin on ALP activity in CVSMCs. Our experiment demonstrated that puerain could attenuate the osteoblastic differentiation of VSMCs through the ER/PI3K-Akt signal pathway.
Asunto(s)
Diferenciación Celular/efectos de los fármacos , Isoflavonas/farmacología , Músculo Liso Vascular/citología , Osteoblastos/citología , Fitoestrógenos/farmacología , Fosfatasa Alcalina/metabolismo , Animales , Aorta/citología , Aorta/metabolismo , Diferenciación Celular/genética , Células Cultivadas , Subunidad alfa 1 del Factor de Unión al Sitio Principal/metabolismo , Estrés del Retículo Endoplásmico/efectos de los fármacos , Estrés del Retículo Endoplásmico/fisiología , Isoflavonas/aislamiento & purificación , Isoflavonas/uso terapéutico , Ratones , Músculo Liso Vascular/efectos de los fármacos , Músculo Liso Vascular/metabolismo , Osteocalcina/metabolismo , Fosfatidilinositol 3-Quinasas/fisiología , Fitoestrógenos/aislamiento & purificación , Fitoestrógenos/uso terapéutico , Fitoterapia , Raíces de Plantas/química , Proteínas Proto-Oncogénicas c-akt/fisiología , Pueraria/química , Transducción de Señal/efectos de los fármacos , Calcificación Vascular/tratamiento farmacológicoRESUMEN
Based on the 1961-2009 weather data from 21 meteorological stations in Ningxia, this paper analyzed the spatiotemporal variation trend of regional agricultural climate resources in Ningxia, Northwest China. In 1961-2009, the air temperature in Ningxia increased gradually from south to north, with the mean annual temperature increased by 0.4 degrees C x (10 a)(-1) , while the annual precipitation in most regions decreased gradually, with a decrement 4.26 mm x (10 a)(-1). Both the frost-free period and the duration of crop growth season prolonged. The regions with > or = 10 degrees C accumulated temperature being > or = 3200 degrees C x d extended southwardly, and thereby, the regions adaptive for planting mid and late rice increased. In 2001-2009, most regions were adaptive for plating winter wheat, and the whole Ningxia was adaptive for plating spring wheat. In the southern mountain regions, the region with mean temperature in July being < or = 20 degrees C decreased gradually, and accordingly, the regions adaptive for planting potato decreased.