RESUMEN
OBJECTIVE@#To explore the role of sinomenine in treatment of allergic rhinitis mice model and its possible mechanism.@*METHOD@#We used ovalbumin (OVA) to make allergic rhinitis model of BALB/c mice. Saline was used in the control group. When we challenged the mice with OVA intranasally, the mice in sinomenine treatment group were feed by the food containing sinomenine. Mice were then killed 24 h after the last OVA challenge. The noses of mice from each group were removed en bloc and fixed, then each section was stained with hematoxylin and eosin. ELISA assay was used to measure the concentration of anti-OVA IgE, IL-4 and IFN-gamma. The proteins expressive level of T-bet and GATA3 were examined.@*RESULT@#Nasal mucosa of the mice in sinomenine treatment group were not hyperplasia and without obvious infiltration of eosinophils. The concentration of anti-OVA IgE, IL-4 and IFN-gamma in the serum and T-bet and GATA3 expression levels of sinomenine treatment group were lower than those of allergic rhinitis group.@*CONCLUSION@#The sinomenine can be used to treat allergic rhinitis mice, and the mechanism may rely on the improvements of the Th1/Th2 imbalance.
Asunto(s)
Animales , Masculino , Ratones , Modelos Animales de Enfermedad , Eosinófilos , Metabolismo , Factor de Transcripción GATA3 , Metabolismo , Inmunoglobulina E , Sangre , Interferón gamma , Sangre , Interleucina-4 , Sangre , Ratones Endogámicos BALB C , Morfinanos , Usos Terapéuticos , Mucosa Nasal , Patología , Fitoterapia , Rinitis Alérgica , Rinitis Alérgica Perenne , Sangre , Quimioterapia , Proteínas de Dominio T Box , Metabolismo , Células TH1 , Células Th2RESUMEN
OBJECTIVE@#To analyze the results of serum in allergic rhinitis and investigate the specific clinic allergen and serum immunoglobulin E (IgE) levels.@*METHOD@#Allergy Screen method was used to detect the specific allergen and total serum IgE level of 134 cases of Allergic rhinitis.@*RESULT@#The dust mite was the most common allergen in inhalation group in 134 cases of allergic rhinitis, the positive rates was 90%; then were donly, feline and scurfy fungus, the positive rates were 16%, 9%. The positive rates of total IgE was 54%. The serum IgE levels between 100 to 200 kU/L, there was 21 cases together, but there existed 7 negative cases. There were 51 cases' IgE levels more than 200 kU/L, the rates was 70.8%, but there still existed 4 negative cases.@*CONCLUSION@#Allergy screen method can find relevant allergen and provide basis for the prevention and treatment of allergic disease.