Comparison of Toxic Metal Concentrations in Antidiabetic Herbal Preparations (ADHPs) Available in Bangladesh Using AAS and XRF Analytical Tools.

Widespread escalation of type 2 diabetes is a concern throughout the world. Developing countries are leading with patients suffering from diabetes-related complications. Plant-based therapeutic, antidiabetic herbal preparations (ADHPs) are being sought for long and the consumption is increasing in in Bangladesh. Plant-based antidiabetic preparations do not go through the screening procedure in terms of safety. Toxic metals in ADHPs have been investigated by two different methods: atomic absorption spectroscopy (AAS) and X-ray fluorescence (XRF). Then, metal concentrations obtained by AAS and XRF were compared. A total of eleven ADHPs were subjected to nondestructive XRF analysis and destructive AAS analysis. Results from the two methods were analyzed statistically by Pearson correlation coefficient (PCC), r xy . Pearson correlation coefficients were found to be -0.05, 0.94, and 1.00 for Mn, Cu, and Zn, respectively. Zn and Cu had significant strong positive correlation (r xy = 1.00 and 0.94, respectively); however, very weak negative correlation was observed in Mn (r xy = -0.05). The concentrations were regressed to observe the presence of linearity. Linear correlation was found for Zn and Cu which indicates a good agreement between AAS and XRF. However, very weak linear correlation in Mn indicates necessitating requirements for further investigation on getting scientific evidence of toxic metal assessment of the antidiabetic herbal preparations for searching and establishing instrumental agreement.

Microbial and Heavy Metal Contaminant of Antidiabetic Herbal Preparations Formulated in Bangladesh.

The aim of the current study was to evaluate microbial contamination in terms of microbial load (total aerobic count and total coliform count) and specific pathogenic bacteria (Salmonella spp., Escherichia coli, particularly Escherichia coli 0157) in thirteen antidiabetic herbal preparations (ADHPs) from Dhaka City. All the thirteen ADHPs had been found contaminated with fungi and different pathogenic bacteria. From the data, it is found that only two of these preparations (ADHP-1 and ADHP-12) complied with the safety limit (as stated in different Pharmacopoeias and WHO guidelines) evaluated by all different microbial counts. None of these herbal preparations could assure the safety as all of them were contaminated by fungi. The overall safety regarding heavy metal content (Zn, Cu, Mn, Cr, Cd, and Pb) was assured as none of them exceeded the safety limit of the daily intake. Microbial contaminants in these herbal preparations pose a potential risk for human health and care should be taken in every step involved in the preparation of these herbal preparations to assure safety.