RESUMEN
Cotton plant provides economically important fiber and cottonseed, but cottonseed contributes 20% of the crop value. Cottonseed value could be increased by providing high value bioactive compounds and polyphenolic extracts aimed at improving nutrition and preventing diseases because plant polyphenol extracts have been used as medicinal remedy for various diseases. The objective of this study was to investigate the effects of cottonseed extracts on cell viability and gene expression in human colon cancer cells. COLO 225 cells were treated with ethanol extracts from glanded and glandless cottonseed followed by MTT and qPCR assays. Cottonseed extracts showed minor effects on cell viability. qPCR assay analyzed 55 mRNAs involved in several pathways including DGAT, GLUT, TTP, IL, gossypol-regulated and TTP-mediated pathways. Using BCL2 mRNA as the internal reference, qPCR analysis showed minor effects of ethanol extracts from glanded seed coat and kernel and glandless seed coat on mRNA levels in the cells. However, glandless seed kernel extract significantly reduced mRNA levels of many genes involved in glucose transport, lipid biosynthesis and inflammation. The inhibitory effects of glandless kernel extract on gene expression may provide a useful opportunity for improving nutrition and healthcare associated with colon cancer. This in turn may provide the potential of increasing cottonseed value by using ethanol extract as a nutrition/health intervention agent.
Asunto(s)
Neoplasias del Colon/tratamiento farmacológico , Gossypium/química , Extractos Vegetales/farmacología , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Neoplasias del Colon/genética , Neoplasias del Colon/metabolismo , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Humanos , ARN Mensajero , Semillas/químicaRESUMEN
Vascular endothelial growth factor (VEGF) plays an important role in chronic inflammation associated with several diseases. Many plant extracts have nutritional and healthy benefits by down-regulating VEGF expression, but there was no report on VEGF regulation by cottonseed extracts in any biological system. The objective was to investigate cell viability and VEGF expression regulated by gossypol and ethanol extracts using lipopolysaccharides (LPS) as a control. MTT, qPCR and immunoblotting techniques were used to monitor cell viability, VEGF mRNA and protein levels in mouse RAW264.7 macrophages. Gossypol dramatically reduced macrophage viability but cottonseed extracts and LPS exhibited minor effect on cell viability. VEGFb mRNA levels were approximately 40 fold of VEGFa in the macrophages. Gossypol increased VEGFa and VEGFb mRNA levels up to 27 and 4 fold, respectively, and increased VEGF protein. LPS increased VEGFa mRNA by sixfold but decreased VEGFb mRNA. LPS increased VEGF protein in 2-4 h but decreased in 8-24 h. Glanded seed extracts showed some stimulating effects on VEGF mRNA levels. Glandless seed coat extract showed increased VEGFb mRNA levels but its kernel extract reduced VEGF mRNA levels. This study demonstrated that gossypol and ethanol extracts differentially regulated cell viability and VEGF expression in mouse macrophages.
Asunto(s)
Gossypium/química , Gosipol/farmacología , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Extractos Vegetales/farmacología , Semillas/química , Factor A de Crecimiento Endotelial Vascular/genética , Secuencia de Aminoácidos , Animales , Supervivencia Celular/efectos de los fármacos , Aceite de Semillas de Algodón , Relación Dosis-Respuesta a Droga , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Gosipol/química , Lipopolisacáridos/farmacología , Ratones , Estructura Molecular , Extractos Vegetales/química , ARN Mensajero/genética , Factor A de Crecimiento Endotelial Vascular/química , Factor A de Crecimiento Endotelial Vascular/metabolismoRESUMEN
Flowering time is a critical trait reflecting the adaptation of plants to their environments. Our initial research has shown that exogenous methyl jasmonate (MeJA) significantly promoted the floret opening of sorghum. To better understand the mechanism of this phenomenon in sorghum, the comparative transcriptome analysis was performed. Transcriptomic analysis showed that the most number of differentially expressed genes was presented between control plants and plants treated with 2.0 mM exogenous MeJA in 2.5 h. A large number of differentially expressed genes were assigned to the subcategory of carbohydrate metabolism and lipid metabolism. The transcriptomic analysis of differentially expressed genes involved in glycolysis/gluconeogenesis and tricarboxylic acid cycle indicated a close relationship between carbohydrates metabolism and flowering. In addition, potassium uptake proteins and aquaporins also played important role in response to the exogenous MeJA in the flowering process. These results provide insights into the effect of MeJA on flowering time and explore the possible molecular mechanism of advancing the flowering period by spraying MeJA. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-021-02743-6.