RESUMEN
Chemical constituents in soft coral Sarcophyton glaucum were separated and purified by various chromatographic methods. Based on the spectral data, physicochemical properties, and comparison with the data reported in the literature, nine cembranoids, including a new cembranoid named sefsarcophinolide(1) together with eight known cembranoids, namely(+)-isosarcophine(2), sarcomilitatin D(3), sarcophytonolide J(4),(1S,3E,7E,13S)-11,12-epoxycembra-3,7,15-triene-13-ol(5), sarcophytonin B(6),(-)-eunicenone(7), lobophytin B(8), and arbolide C(9), were identified. As revealed by biological activity experiment results, compounds 2-6 had weak acetylcholinesterase inhibitory activity, and compound 5 displayed weak cytotoxicity against K562 tumor cell line.
Asunto(s)
Antozoos , Animales , Acetilcolinesterasa , Línea Celular TumoralRESUMEN
Two new triterpenoid saponins (1 and 2), together with two known saponins (3 and 4) were isolated from Bupleurum marginatum Wall. ex DC. Their structures were elucidated by the comprehensive spectroscopic analysis. Compounds 1 and 2 represented the rare example of an oleanane-type triterpenoid with two sugar moieties at C-3 and C-28. The cytotoxic activity of four compounds was evaluated against normal heptocell BRL-3A in vitro.
RESUMEN
One new monoterpenoid, nerol oxide-8-carboxylic acid (1), and one new flavonoid glycoside, claulansoside A (2), together with six known compounds, clausenamide (3), quercetin (4), isorhamnetin (5), dihydromyric (6), 2",3"-dihydroxyanisolactone (7) and (E,E)-8-(7-hydroxy-3,7-dimethylocta-2,5-dienyloxy)psoralen (8), have been isolated from the peels of Clausena lansium (Lour.) Skeels. Their structures were determined using a combination of 1D, and 2D NMR (HMQC, HMBC, COSY and NOESY) techniques, and HR-ESI-MS analyses. Compounds 1 and 7 exhibited antibacterial activity against Staphylococcus aureus with the diameter of inhibition zones of 11.5 mm and 14.2 mm. Compounds 3 and 6 showed α-glucosidase inhibitory activity in vitro.
Asunto(s)
Clausena/química , Flavonoides/aislamiento & purificación , Monoterpenos/aislamiento & purificación , Monoterpenos Acíclicos , Flavonoides/química , Frutas/química , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Monoterpenos/químicaRESUMEN
A new degraded sesquiterpene, tectoionol C (1), together with five known compounds wilsonol F (2), heterodendrin (3), epi-heterodendrin (4), 7-deoxogeayine (5), and 1H-indole-3-carboxylicacid, methyl ester (6), was isolated from the twigs of Trigonostemon lutescens. Their structures were determined by spectroscopic methods including 1D and 2D NMR techniques, as well as by comparison with published data.
Asunto(s)
Euphorbiaceae/química , Sesquiterpenos/química , Modelos Moleculares , Estructura MolecularRESUMEN
One new diterpenoid, 11alpha,12betaH-dolabella-4,8(17)-dien-3alpha,7beta,18-triol (1) and one new sesquilignan, 9-methoxy-7',8'-cis-7",8"-cis-buddlenol B (2), together with three known compounds, (+)-diasyringaresinol (3), N-methyl-5- hydroxy-delta3-pyrrolin-2-one (4) and marmin (5), have been isolated from Aglaia odorata var. microphyllina. Their structures were determined using 1D and 2D NMR spectroscopy. Compound 1 exhibited cytotoxic activity against the K562 cell line with an IC50 value of 12.5 microg/mL.
Asunto(s)
Aglaia/química , Diterpenos/aislamiento & purificación , Diterpenos/química , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Células K562 , Estructura Molecular , Plantas Medicinales/químicaRESUMEN
Agarwood is the fragrant resinous heartwood obtained from certain trees in the genus Aquilaria belonging to the family Thymelaeaceae. 2-(2-Phenylethyl)chromones and characteristic sesquiterpenes are the main classes of aromatic compounds isolated from agarwood. Although there are many sesquiterpenes, relatively few 2-(2-phenylethyl)chromones have been determined in agarwood by GC-MS. After analysis of the MS spectra of eighteen 2-(2-phenylethyl)chromone derivatives isolated from agarwood and identified by NMR spectroscopy, together with the reported MS data and characteristic of structures of 2-(2-phenylethyl)chromones, the MS characterization, fragmentation patterns and characteristic fragment peaks for the compounds were deduced and a table summarizing MS characterization of 2-(2-phenylethyl)chromones in agarwood is presented. All the 2-(2-phenylethyl)chromones previously reported in agarwood are substituted by methoxy or/and hydroxy groups, except for one compound. Due to the fact they all possess the same basic skeleton (molecular weight: 250) and similar substituent groups (methoxy or hydroxy groups), a formula (30m + 16n = MW - 250) is provided to calculate the number of methoxy (m) or hydroxy (n) groups according to molecular ion peak or molecular weight (MW). We deduced that the characteristic fragmentation behaviors of the 2-(2-phenylethyl)chromones are the cleavages of the CH2-CH2 bond between chromone moiety and phenyl moiety. Thus, characteristic fragment ions, such as m/z 91 [C7H7], 107 [C7H6+OH], 121 [C7H6+OCH3], 137 [C7H5+OH+OCH3] are formed by different substituted benzyl moieties, while characteristic fragment ions such as m/z 160 [C10H8O2], 176 [C10H7O2+OH], 190 [C10H7O2+OCH3], 220 [C10H6O2+OCH3×2] are formed by different substituted chromone moieties. Furthermore, rules regarding to the relationship between the positions of hydroxy or methoxy groups and the relative abundances of benzyl and chromone fragment ions have been deduced. Elucidation of how the positions of hydroxy or methoxy groups affect the relative abundances of benzyl and chromone fragment peaks is also provided. Fifteen unidentified compounds of an artificial agarwood sample analyzed by GC-MS, were preliminary determined as 2-(2-phenylethyl)chromones by analysis of their MS characterization and by comparison of their MS spectra with those of 18 standard compounds or 2-(2-phenylethyl)chromones reported in literature according to the above-mentioned methods and rules. This report will be helpful for the analysis and structural elucidation of 2-(2-phenylethyl)chromones in agarwood by GC-MS, and provides fast and reliable characterization of the quality of agarwood.
Asunto(s)
Flavonoides/química , Thymelaeaceae/química , Madera/química , Fraccionamiento Químico , Flavonoides/aislamiento & purificación , Cromatografía de Gases y Espectrometría de Masas , Peso Molecular , Ácido Palmítico/química , Ácido Palmítico/aislamiento & purificación , Aceites de Plantas/química , Aceites de Plantas/aislamiento & purificaciónRESUMEN
Five new 2-(2-phenylethyl)chromone derivatives, qinanones A-E (1-5), together with eight known 2-(2-phenylethyl)chromone derivatives (6-13), were isolated from the Et2O extract of high-quality Chinese agarwood "Qi-Nan" originating from Aquilaria sinensis. The structures of the new 2-(2-phenylethyl)chromones were elucidated by spectroscopic techniques (UV, IR, 1D and 2D NMR) and MS analyses. In the bioassay for acetylcholinesterase inhibitors, compounds 1-6, 10, and 12 exhibited weak inhibitory activities (inhibition percentage ranged from 10 % to 24 % at the concentration of 50 µg/mL). Compared with other agarwoods, "Qi-Nan" was different in containing 2-(2-phenylethyl)chromones with unsubstituted chromone rings.
Asunto(s)
Acetilcolinesterasa/metabolismo , Inhibidores de la Colinesterasa/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Flavonoides/aislamiento & purificación , Thymelaeaceae/química , Inhibidores de la Colinesterasa/química , Inhibidores de la Colinesterasa/farmacología , Medicamentos Herbarios Chinos/farmacología , Flavonoides/química , Flavonoides/farmacología , Estructura Molecular , QiRESUMEN
Two new acridone alkaloids, 3-methoxy-1,4,5-trihydroxy-10-methylacridone (1) and 2,3-dimethoxy-1,4,5-trihydroxy-10-methylacridone (2), were isolated from the ethanol extract of the branch of Atalantia buxifolia. Their structures were elucidated by spectroscopic methods including 1D and 2D NMR. Compounds 1 and 2 exhibited significant antibacterial activity against Staphylococcus aureus and weak inhibitory effect on acetylcholinesterase.
Asunto(s)
Acridinas/aislamiento & purificación , Acridinas/farmacología , Alcaloides/aislamiento & purificación , Alcaloides/farmacología , Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Inhibidores de la Colinesterasa/aislamiento & purificación , Inhibidores de la Colinesterasa/farmacología , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Rutaceae/química , Acetilcolinesterasa , Acridinas/química , Acridonas , Alcaloides/química , Antibacterianos/química , Inhibidores de la Colinesterasa/química , Medicamentos Herbarios Chinos/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Raíces de Plantas/química , Staphylococcus aureus/efectos de los fármacosRESUMEN
A new denitroaristolochic acid, demethylaristofolin C (1), together with six known alkaloids, crebanine N-oxide (2), (-)-sukhodianine-ß-N-oxide (3), palmatine (4), corydalmine (5), dehydrocorydalmine (6), and corynoxidine (7), was isolated from the tubers of Stephania succifera. The structure of demethylaristofolin C was elucidated by spectroscopic techniques (UV, IR, 1D, and 2D NMR) and HR-ESI-MS analyses. These compounds exhibited antibacterial activities against Staphylococcus aureus and methicillin-resistant S. aureus strains in different degrees.
Asunto(s)
Alcaloides/aislamiento & purificación , Alcaloides/farmacología , Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Fenantrenos/aislamiento & purificación , Fenantrenos/farmacología , Stephania/química , Alcaloides/química , Antibacterianos/química , Berberina/análogos & derivados , Alcaloides de Berberina , Medicamentos Herbarios Chinos/química , Resistencia a la Meticilina/efectos de los fármacos , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Fenantrenos/química , Tubérculos de la Planta/química , Staphylococcus aureus/efectos de los fármacosRESUMEN
A new sesquiterpene lactone, named tomenphantopin H (1), together with two known germacranolides, 2ß-methoxy-2-deethoxy-8-O-deacylphantomolin-8-O-tiglinate (2) and 2-deethoxy-2-hydroxyphantomolin (3), was isolated from the whole plant of Elephantopus tomentosus Linn. The new compound was completely elucidated using a combination of 1D and 2D NMR techniques (COSY, HMQC, and HMBC) and HR-ESI-MS analyses. All compounds exhibited antibacterial activity.
Asunto(s)
Antibacterianos/aislamiento & purificación , Asteraceae/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Lactonas/aislamiento & purificación , Sesquiterpenos/aislamiento & purificación , Antibacterianos/química , Antibacterianos/farmacología , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Lactonas/química , Lactonas/farmacología , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Sesquiterpenos/química , Sesquiterpenos/farmacología , Sesquiterpenos de Germacrano/química , Sesquiterpenos de Germacrano/aislamiento & purificaciónRESUMEN
A new tetranortriterpenoid, named 6-deacetyl-severinolide, together with six known tetranortriterpenoids severinolide, acetyl-isoepiatalantin, 7-isovaleroylcycloepiatalantin, cycloepiatalantin, 7-isovaleroylcycloseverinolide, and atalantin, was isolated from the ethanol extract of the roots of Atalantia buxifolia collected in Hainan. Their structures were elucidated by spectroscopic methods including 1D and 2D NMR (HMQC, ¹H-¹H COSY, HMBC, and NOESY).
Asunto(s)
Medicamentos Herbarios Chinos/aislamiento & purificación , Limoninas/aislamiento & purificación , Rutaceae/química , Medicamentos Herbarios Chinos/química , Limoninas/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Raíces de Plantas/químicaRESUMEN
Two new triterpene saponins, ilekudinchosides F (1) and G (2), along with three known saponins were isolated from the leaves of Ilex kudingcha C. J. Tseng. The new compounds were characterized as 3ß,19α-dihydroxy-12α-ethoxy-urs-13(18)-ene-28,20ß-lactone-3-O-[ß-D-glucopyranosyl(1 â 3)]-[α-L-rhamnopyranosyl(1 â 2)]-α-L-arabinopyranoside (1) and 3ß,19α-dihydroxy-12α-methoxy-urs-13(18)-ene-28,20ß-lactone-3-O-[α-L-rhamnopyranosyl(1 â 2)]-α-L-arabinopyranoside (2). The new structures were elucidated by spectroscopic methods including 1D and 2D NMR, HR-TOF-MS, and CD spectrometry, and the known compounds were identified by the comparison of their NMR and HR-TOF-MS data with those reported in the literature.
Asunto(s)
Medicamentos Herbarios Chinos/aislamiento & purificación , Ilex/química , Saponinas/aislamiento & purificación , Triterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Hojas de la Planta/química , Saponinas/química , Triterpenos/químicaRESUMEN
A new diterpene, named yucalexin P-23 (1), together with three known compounds, yucalexin P-15 (2), protocatechuic acid (3), and catalpinic acid (4), was isolated from the stems of Manihot esculenta. Their structures were determined by means of physicochemical evidence and spectral analysis. Compounds 3 and 4 exhibited antimicrobial activity.
Asunto(s)
Diterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Manihot/química , Diterpenos/química , Diterpenos/farmacología , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Tallos de la PlantaRESUMEN
Chemical studies on the constituents from the dragon's blood of Dracaena cambodiana led to the discovery of three new flavonoid derivatives (1- 3) and six known compounds (4- 9). The structures of the three new compounds were elucidated by NMR and MS spectroscopic analyses. All compounds were evaluated for their growth inhibitory activity against human cell lines K-562, SMMC-7721, and SGC-7901, as well as antibacterial activities against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). As a result, compounds 1, 2, 5, 7, and 9 showed cytotoxicity against K-562, SMMC-7721, and SGC-7901 cell lines. All these compounds were observed to exhibit antibacterial activities against S. aureus, and compounds 1- 4, 6, 8, and 9 were observed to exhibit antibacterial activity against MRSA.
Asunto(s)
Antibacterianos/aislamiento & purificación , Dracaena/química , Flavonoides/química , Antibacterianos/química , Antibacterianos/farmacología , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Línea Celular Tumoral , Flavonoides/aislamiento & purificación , Flavonoides/farmacología , Humanos , Espectroscopía de Resonancia Magnética , Espectrometría de Masas/métodos , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Estructura Molecular , Staphylococcus aureus/efectos de los fármacosRESUMEN
A new drimane sesquiterpenoid glycoside, named 7-drimen-3ß,11-diol 3-O-ß-d-glucopyranoside, was isolated from the 95% EtOH extract of the seeds of Antiaris toxicaria (Pers.) Lesch. The chemical structure was completely elucidated using a combination of 1D and 2D NMR techniques (COSY, HMQC, HMBC, and ROESY) and HR-ESI-MS analysis. The compound showed inhibitory activities toward methicillin-resistant Staphylococcus aureus (MRSA), chronic myelogenous leukemia (K562), and human hepatoma (SMMC-7721) cell lines.
Asunto(s)
Antiaris/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Glucósidos/aislamiento & purificación , Sesquiterpenos/aislamiento & purificación , Antiinfecciosos , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Glucósidos/química , Glucósidos/farmacología , Humanos , Células K562 , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Resonancia Magnética Nuclear Biomolecular , Sesquiterpenos Policíclicos , Semillas/química , Sesquiterpenos/química , Sesquiterpenos/farmacologíaRESUMEN
Bioassay-guided fractionation of the ethanolic extract from the seeds of Antiaris toxicaria led to the isolation of three new cardiac glycosides named toxicarioside J, toxicarioside K, and toxicarioside L, together with a known glucostrophalloside. The structures of the new compounds were elucidated by spectroscopic methods including HRESIMS, UV, IR, and 1D, 2D NMR techniques. The cytotoxic activities of these cardiac glycosides against human gastric (SGC-7901) and human hepatoma (SMMC-7721) cell lines were evaluated, and all of them exhibited significant cytotoxicity.
Asunto(s)
Antiaris/química , Antineoplásicos Fitogénicos/farmacología , Cardenólidos/farmacología , Glicósidos Cardíacos/farmacología , Extractos Vegetales/farmacología , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Bioensayo , Cardenólidos/química , Cardenólidos/aislamiento & purificación , Glicósidos Cardíacos/química , Glicósidos Cardíacos/aislamiento & purificación , Línea Celular Tumoral , Supervivencia Celular , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Concentración 50 Inhibidora , Espectroscopía de Resonancia Magnética , Medicina Tradicional de Asia Oriental , Estructura Molecular , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Plantas Medicinales/química , Semillas/químicaRESUMEN
The investigation of chemical constituents from the branches of Calophyllum inophyllum Linn led to the isolation of a new prenylated xanthone, named caloxanthone Q (1), together with three known compounds, 2-deprenylrheediaxanthone B (2), jacareubin (3), and 6-deoxyjacareubin (4). Their structures were completely elucidated on the basis of spectroscopic methods (UV, IR, HR-ESI-MS, 1D NMR, and 2D NMR).
Asunto(s)
Calophyllum/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Xantonas/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Humanos , Células K562 , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Tallos de la Planta/química , Piranos/química , Piranos/aislamiento & purificación , Xantenos/química , Xantenos/aislamiento & purificación , Xantonas/químicaRESUMEN
Two new 2-(2-phenylethyl)chromones, (5S(*),6R(*),7S(*))-5,6,7-trihydroxy-2-(3-hydroxy-4-methoxyphenethyl)-5,6,7,8-tetrahydro-4H-chromen-4-one (1) and (5S(*),6R(*),7R(*))-5,6,7-trihydroxy-2-(3-hydroxy-4-methoxyphenethyl)-5,6,7,8-tetrahydro-4H-chromen-4-one (2), were isolated from the Chinese eaglewood of Aquilaria sinensis (Lour.) Gilg. Their structures were established by detailed MS and NMR spectroscopic analysis, as well as comparison with the literature data.
Asunto(s)
Cromonas/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Flavonoides/aislamiento & purificación , Thymelaeaceae/química , Cromonas/química , Medicamentos Herbarios Chinos/química , Flavonoides/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , EstereoisomerismoRESUMEN
Chemical investigation of the ethanol extract of the aerial parts of Scyphiphora hydrophyllacea Gaertn.collected in Hainan Province of China resulted in the isolation of a new iridoid, scyphiphin C (1) and a known iridoid glycoside, shanzhiside methyl ester (2). Their structures were elucidated by a study of their physical and spectral data.
Asunto(s)
Helechos/química , Iridoides/química , Etanol/química , Iridoides/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Estructura Molecular , Extractos Vegetales/químicaRESUMEN
Volatile oils of five different kinds of Chinese eaglewood were extracted with aether at room temperature. The chemical constituents and relative contents of the volatile oils were analysed by GC-MS. It showed that all the five volatile oils were mainly composed of sesquiterpenes, aromatic constituents and fatty acids. Several sesquiterpenes, such as hinesol, nootkatone, valerenic acid, velleral, guaiol, gamma-gurjunene, gamma-selinene, viridiflorol, isoaromadendrene epoxide, valencene, alpha-costol et. al., together with several aromatic constituents, 2,4-di-tert-butylphenol,4-methyl-2,6-di-tert-butylphenol, phenylpropionic acid, 1-(benzyloxy)-8-naphthol, anisylacetone, et. al. were found in the volatile oils of Chinese eaglewood for the first time. The samilarities and differences of the volatile oils from the five kinds of Chinese eaglewood were compared. It suggested that the quality of Chinese eaglewood could be evaluated by GC-MS analyse of the volatile oil.