RESUMEN
Chemical investigation of the insect-derived actinomycete Amycolatopsis sp. HCa1 with NMR-guided fractionation led to the isolation of eight new tetrasaccharide derivatives containing an unusual nonreducing glucotetraose skeleton, named actinotetraoses A-H (1-8), and one known compound, tigloside (9). The structures of these compounds were determined on the basis of analyses of their spectroscopic data. The in vitro immunosuppressive activity and cytotoxicity of these compounds were evaluated by T cell viability and MTT assays, respectively. Only actinotetraose E (5) displayed weak immunosuppressive activity.
Asunto(s)
Actinobacteria/química , Saltamontes/microbiología , Inmunosupresores/aislamiento & purificación , Oligosacáridos/aislamiento & purificación , Linfocitos T/efectos de los fármacos , Actinobacteria/aislamiento & purificación , Animales , Supervivencia Celular/efectos de los fármacos , Ensayos de Selección de Medicamentos Antitumorales , Fermentación , Células HeLa , Células Hep G2 , Humanos , Inmunosupresores/química , Inmunosupresores/farmacología , Espectroscopía de Resonancia Magnética , Estructura Molecular , Micelio/química , Oligosacáridos/química , Oligosacáridos/farmacología , Rotación ÓpticaRESUMEN
Two new polyketides, arthropsadiol C (1) and massarilactone H (2), together with six known derivatives (3-8) were isolated from the culture broth of the marine-derived fungus Phoma herbarum. Their structures were elucidated on the basis of spectroscopic methods, including 2D NMR techniques. Compounds 2, 4, 5, and 8 showed moderate neuraminidase inhibitory activity with IC(50) values ranging from 4.15 to 9.16 µM.