RESUMEN
In this study, the composition of fatty oil from Semen Ziziphi Spinosae and its cardiotonic activity on the heart isolated from a toad were studied. Oil-in-water (O/W) emulsions of fatty oil were prepared by the perfusion method. The fatty oil had a positive inotropic effect on isolated rat hearts at a concentration between 5 × 10(-3) and 2 × 10(-2) mL/10 mL, and the effect was in positive correlation with the concentration of calcium ions. In addition, this effect was inhibited by 2 mg/mL nifedipine, suggesting that the cardiotonic mechanism could be responsible for accelerating the inflow of calcium ions. Gas chromatography-mass spectrometry analysis showed that the main constituents of the fatty oil were 9-octadecenoic acid (43.32%), 9,12-octadecadienoic acid (42.57%), hexadecanoic acid (4.76%), 9-eicosenoic acid (2.95%), stearic acid (2.41%) and arachidic acid (0.81%). This preliminary study revealed that the fatty oil of Semen Ziziphi Spinosae exhibited remarkable cardiotonic activity in the tested models, and it is necessary to further reveal the effective substances of the fatty oil.
Asunto(s)
Corazón/efectos de los fármacos , Aceites de Plantas/farmacología , Animales , Anuros , Ácidos Eicosanoicos/química , Cromatografía de Gases y Espectrometría de Masas , Técnicas In Vitro , Ácido Linoleico/química , Miocardio/metabolismo , Ácido Oléico/química , Ácido Palmítico/química , Aceites de Plantas/química , Ácidos Esteáricos/química , Resistencia a la Tracción/efectos de los fármacosRESUMEN
This study aims to explore and evaluate the effects of Semen Ziziphi Spinosae extracts on the serum levels of interleukin (IL)-6, IFN-γ, IL-1ß, TNF-α, IL-10 and IL-4 in mice, and the regulative effect of Semen Ziziphi Spinosae on the cytokine system. Using an ELISA assay, the serum levels of IL-6, IFN-γ, IL-1ß, TNF-α, IL-10 and IL-4 were examined in mice after intraperitoneal injection (i.p.) with Semen Ziziphi Spinosae extracts. The results showed that the levels of IL-6 and IL-1ß were significantly increased compared with the control groups (p<0.01), while the level of tumour necrosis factor-α (TNF-α) was significantly decreased (p<0.01). Semen Ziziphi Spinosae possesses certain modulation effects on cytokines, and the immuno-regulating function and hypnosis effects of Semen Ziziphi Spinosae may be relevant to these modulation effects on cytokines.
Asunto(s)
Interferón gamma/sangre , Interleucina-10/sangre , Interleucina-1beta/sangre , Interleucina-4/sangre , Interleucina-6/sangre , Extractos Vegetales/farmacología , Factor de Necrosis Tumoral alfa/sangre , Ziziphus/química , Animales , Ensayo de Inmunoadsorción Enzimática , Pérdida de Tono Postural/efectos de los fármacos , Ratones , Extractos Vegetales/químicaRESUMEN
A reverse phase high performance liquid chromatographic (HPLC) method with evaporative light scattering detection (ELSD) was developed for simultaneous determination of jujuboside A, B and betulinic acid in semen Ziziphi spinosae. The analysis was performed by gradient elution, using an aqueous mobile phase (containing 0.1% acetic acid) modified by acetonitrile. The evaporator tube temperature of ELSD was set at 45 degrees C, and with the nebulizing gas flow-rate of 1.8l/min. The method was validated for accuracy, reproducibility, precision and limits of detection and quantification. Quantification of the three active compounds in semen Ziziphi spinosae from different locations was performed by this method, which provides a new tool for quality assessment of semen Ziziphi spinosae.