Dietary squalene supplementation alleviates diquat-induced oxidative stress and liver damage of broiler chickens.

The aim of this study was to explore the protective effects of squalene supplementation on growth performance, oxidative status, and liver function of diquat-challenged broilers. One hundred forty-four 1-day-old male Ross 308 broiler chicks were allocated to 3 groups, and each group consisted of 6 replicates of 8 birds each. The three groups were as follows: 1) nonchallenged broilers fed with a basal diet (control group), 2) diquat-challenged broilers fed a basal diet, and 3) diquat-challenged broilers fed with a basal diet supplemented with 1.0 g/kg of squalene. Broilers were intraperitoneally injected with 20 mg/mL of diquat solution at a dosage of 1 mL/kg of BW or an equivalent amount of saline at 20 d. Compared with the control group, weight gain and BW change rate during 24 h after injection were decreased by diquat challenge (P < 0.05), and the diquat-induced compromised growth performance was improved by squalene supplementation (P < 0.05). Diquat administration reduced plasma superoxide dismutase activity and increased malondialdehyde accumulation and glutathione peroxidase activity in both plasma and the liver (P < 0.05). In contrast, plasma glutathione peroxidase activity in diquat-challenged broilers was reduced by squalene supplementation (P < 0.05). The hepatic glutathione level was reduced by diquat administration (P < 0.05), whereas its level in plasma and the liver of diquat-challenged broilers was increased by squalene supplementation (P < 0.05). The relative liver weight of broilers was increased by diquat challenge (P < 0.05), with its value being intermediate in the squalene-supplemented group (P > 0.05). The plasma aminotransferase activities and total bilirubin concentration were increased by diquat challenge (P < 0.05), which were reduced by squalene supplementation (P < 0.05). The mRNA abundance of hepatic nuclear factor erythroid 2-related factor 2 (P < 0.05) was upregulated by diquat treatment, regardless of squalene supplementation. The mRNA abundance of hepatic glutathione peroxidase 1 and B-cell lymphoma/leukemia 2-associated X protein was upregulated by diquat challenge (P < 0.05), which was reversed by squalene administration (P < 0.05). Squalene increased NAD(P)H quinone dehydrogenase 1 mRNA abundance and decreased caspase 3 mRNA abundance in the liver of diquat-challenged broilers (P < 0.05). The results suggested that squalene can increase weight gain, improve oxidative status, and alleviate liver injury in diquat-challenged broilers.

[Studies permeation of puerarin and its phospholipid complex through mouse skin in vitro].

OBJECTIVE: To investigate the skin permeation of puerarin and its phospholipid complex and compare the differences between their permeation rates and cumulative permeation amounts. METHOD: Performing a test of permeation through the mouse skin in vitro in an improved Franz diffusion cell. RESULT: The cumulative permeation amount of phospholipid complex was higher than that of puerarin in the first hour and then increased slowly, meanwhile the permeation rate of puerarin rose higher than that of the complex. CONCLUSION: Phospholipid complex of puerarin can permeate through the mouse skin rapidly up to a certain amount in a short time, then begins to release drug slowly and lastingly.

[Studies on constituents of Ilex chinensis Sims].

Several compounds characterised as protocatechuic acid, caffeic acid, syringin, rotundic acid and pedunculoside were isolated from the leaves of Ilex chinensis, a Chinese crude drug. A novel compound cyclohexanone pedunculosyl-3,23-O-acetal was identified and proposed as a pedunculoside derivative produced in the extracting procedure.

Glucosylsterols in extracts of Euryale ferox identified by high resolution NMR and mass spectrometry.

The structures of three glucosylsterols in a glycolipid mixture from the product of the naturally dried medicinal plant Euryale ferox Salisb. have been elucidated by 500 MHz nuclear magnetic resonance and mass spectroscopic methods and characterized as follows: 24-methylcholest-5-enyl-3 beta-O-pyranoglucoside, 24-ethylcholest-5-enyl-3 beta-O-pyranoglucoside, and 24-ethylcholesta-5,22E-dienyl-3 beta-O-pyranoglucoside. These constituents may be the active substances of the medicinal plant.