RESUMEN
Arsenic (As) pollution is a serious and longstanding problem, which has obvious threaten to aquatic organisms. The study aimed to explore the mitigation effect of natural antioxidant zinc (Zn) on As toxicity in the foregut and midgut of common carp (Cyprinus carpio L.), and in-depth disclose related signal cascade. Carps were treated with Zn2+ (1 mg/L) and/or As3+ (2.83 mg/L) for a period of 30 days. Under As exposure, the foregut and midgut showed obvious burst of reactive oxygen species (ROS) and breakdown of antioxidant system. What followed is the activation of the endogenous and exogenous apoptotic pathways, and the rise of autophagy level prompted by the increase in LC3 II and the down-regulation of p62. Mitochondrial swelling, cristae fragmentation and autophagosomes were observed under the electron microscope, which also means the occurrence of apoptosis and autophagy. In addition, As induced the activation of p38 mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK) and the inhibition of extracellular signal-related kinase (ERK) in MAPK signaling, and up-regulated the level of autophagy through the inhibition of the phosphatidylinositol 3 kinase (PI3K)/AKT/ mammalian target of rapamycin (mTOR) signaling cascade. However, Zn supplementation has clearly reversed the above phenomenon, and it basically has no effect on foregut and midgut. In conclusion, this study shows that Zn can alleviate the damage caused by subchronic As exposure, which provides a reference for the use of Zn preparations in aquaculture.
Asunto(s)
Intoxicación por Arsénico , Carpas , Contaminantes Químicos del Agua , Animales , Apoptosis , Quinasas MAP Reguladas por Señal Extracelular , Fosfatidilinositol 3-Quinasa , Fosfatidilinositol 3-Quinasas , Proteínas Proto-Oncogénicas c-akt , Especies Reactivas de Oxígeno , Serina-Treonina Quinasas TOR , Contaminantes Químicos del Agua/toxicidad , ZincRESUMEN
Arsenic pollution is a common threat to aquatic ecosystems. The effects of chronic exposure to arsenite on the brains of aquatic organisms are unknown. This study was designed to evaluate arsenic-induced brain damage in common carp (Cyprinus carpio) and the ameliorating effects of divalent zinc ion (Zn2+) supplementation from the aspects of oxidative stress (OxS), tight junction (TJ), apoptosis and autophagy. After arsenite exposure (2.83 mg/L) for 30 days, oxidative damage to the brain was determined, as indicated by inhibited antioxidants system (catalase-superoxide dismutase system, and glutathione system) and elevated levels of biomacromolecule peroxidation (malondialdehyde and 8-hydroxydeoxyguanosine). Moreover, we also found functional damage to the brain as suggested by injuries to the blood-brain barrier (decreases in tight junction) and nerve conduction (depletion of AChE). Mechanisticly, apoptotic and autophagic cell death were indicated by typical morphologies including karyopyknosis and autophagosome, accompanying by key bio-indicators (Bcl-2, caspase and autophagy related gene family proteins). In contrast, the coadministration of Zn2+ (1 mg/L) with arsenite effectively alleviated this damage as suggested by the recovery of the aforementioned bioindicators. This study provides new insight into the brain toxicity caused by arsenite and suggests the application of zinc preparations in the aquatic pollution of arsenic.
RESUMEN
Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health. However, the toxicity of antibiotics on aquatic organisms, especially the effects on the detoxification system and immune system, has not been thoroughly studied. Lycopene (LYC) is a naturally occurring hydrocarbon carotenoid, which has received extensive attention as a potential antioxidant. The aim of this study was to investigate whether LYC alleviates exogenous toxicity in carp induced by sulfamethoxazole (SMZ) and the underlying molecular mechanisms. The grass carp were treated with SMZ (0.3 µg L-1) and/or LYC (10 mg per kg body weight) for 30 days. Indexes, such as hepatic function-related including histopathological changes and biochemical parameters, detoxification system-related including the cytochrome P450 enzyme system and antioxidant system, and immune system-related including inflammatory and apoptosis processes were detected. The results showed that SMZ stress leads to significant pathological damage of the liver and induction of oxidative stress. LYC coadministration recovered the cytochrome p450-1A1 homeostasis and decreased SMZ-induced accumulation of intracellular reactive oxygen species (ROS). Mechanistically, indicators in the innate immune system (such as toll like receptors (TLRs), tumor necrosis factor-α (TNF-α), interleukin (IL)-1ß, IL-6 and IL-8) and the apoptosis pathway (p53, PUMA, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax), and Caspase-9/3) disclosed adaptive activation under SMZ exposure; these anomalies returned to normal or close-to-normal levels after LYC coadministration. Therefore, LYC dietary supplement possesses liver protective function against exogenous toxic compounds like SMZ, making LYC a functional aquatic feed ingredient for aquiculture.
Asunto(s)
Antioxidantes/farmacología , Carpas , Hígado/efectos de los fármacos , Licopeno/farmacología , Sulfametoxazol/toxicidad , Animales , Apoptosis/efectos de los fármacos , Carpas/metabolismo , Citocromo P-450 CYP1A1/metabolismo , Citocinas/genética , Citocinas/metabolismo , Mediadores de Inflamación/metabolismo , Hígado/metabolismo , Hígado/patología , Mitocondrias Hepáticas/efectos de los fármacos , Mitocondrias Hepáticas/metabolismo , Mitocondrias Hepáticas/ultraestructura , Estrés Oxidativo/efectos de los fármacos , Mapas de Interacción de Proteínas , Especies Reactivas de Oxígeno/metabolismo , Receptores Toll-Like/metabolismoRESUMEN
The potential antagonistic mechanism between zinc (Zn) and arsenic (As) on renal toxicity was investigated in common carp. The results showed that by increased Zn efflux and retention (as reflected by zinc transporter 1 (ZnT-1), Zrt- and Irt- 1ike protein (ZIP) and metallothionein (MT) expression), Zn co-administration significantly recovered the antioxidant function (catalase, CAT) and the level of renal barrier function (Occludin, Claudins and Zonula Occludens) in comparison to As treatment. Interestingly, Zn co-administration with As resulted in carps undergoing reduction of heat shock response (HSPs), a low induction of autophagy flux (Beclin-1, microtubule-associated protein 1 light chain 3 (LC3) and sequestosome 1 (P62)) and decreased endoplasmic reticulum (ER) stress (activating transcription factor 6 (ATF-6), inositol requiring-1α (IRE1) and PKR-like ER kinase (PERK)) in the aspect of mRNA or protein levels. All these alleviated protein quality control processes induced by Zn under As stress was correlated with the no longer loosen tight connection, less swollen endoplasmic reticulum as well as reduced formation of autophagosomes and autophagic vesicles. Mechanically, post-transcriptional regulated protein quantities compromising phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway was demonstrated true causative forces inside the cell for Zn against As poisoning. In conclusion, we suggested the potential renal protective effect of Zn supplementation against As exposure by the modulation of protein quality control processes.
Asunto(s)
Arsénico , Carpas , Animales , Apoptosis , Arsénico/toxicidad , Autofagia , Estrés del Retículo Endoplásmico , Zinc/toxicidadRESUMEN
Epidemiological and laboratory investigations have extensively indicated that arsenic exposure accounts for several kidney diseases. Zinc has been suggested as a possible natural preventive and therapeutic agent. This study is designed to explore the beneficial effect of zinc supplementation against arsenic-induced renal toxicity in common carp, and the results point to signaling pathway possibly compromised. In the present study, renal injury was induced in common carp by waterborne exposure to arsenic (2.83 mg/L) for 30 days, and zinc (1 mg/L) was simultaneously supplemented. First, the arsenic-exposed fish showed histological and functional renal alterations (indicated by hematoxylin-eosin staining, biochemical indexes and a TUNEL assay). Moreover, as a reactive oxygen species (ROS) stimulant, arsenic was found to induce oxidative toxicity as determined by increased renal ROS, malondialdehyde, protein carbonyl and 8-hydroxydeoxyguanosine levels. When antioxidant-mediation attempts (through superoxide dismutase and glutathione)-mediated to restore homeostasis failed and ROS increased to extreme levels, inflammation (indicated by elevated inducible nitric oxide synthetase, tumor necrosis factor-alpha and interleukins levels) and apoptosis (through both mitochondrial- and death receptor-dependent pathways) were triggered. However, abnormalities in the upstream mediators Nrf2, NF-κB and MAPK were significantly ameliorated and blocked by treatment with zinc. In conclusion, zinc exerts a substantial protective effect against arsenic-triggered subchronic renal injury in common carp via the amelioration of oxidative stress, suppression of apoptosis and reduced inflammation through Nrf2, NF-κB and MAPK signaling.
Asunto(s)
Antiinflamatorios/farmacología , Antioxidantes/farmacología , Arsénico/toxicidad , Carpas/metabolismo , Riñón/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Zinc/farmacología , Animales , Apoptosis/efectos de los fármacos , Proteínas de Peces/metabolismo , Riñón/metabolismo , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Factor 2 Relacionado con NF-E2/metabolismo , FN-kappa B/metabolismo , Estrés Oxidativo/efectos de los fármacos , Transducción de Señal/efectos de los fármacosRESUMEN
Arsenic and copper, two toxic pollutants, are powerful inducers of oxidative stress. Exposure to copper and arsenic can cause intestinal injury in cockerel. This study was carried out to investigate the effects of these two pollutants on the gastrointestinal tract of cockerels. Experimental results showed that the activity of antioxidant enzymes (catalase and glutathione peroxidase) was inhibited and the ionic balance was destroyed after exposure to copper sulfate (300 mg/kg) and/or arsenic trioxide (30 mg/kg). However, the expression of pro-inflammatory cytokines (nuclear factor kappa-B, cyclooxygenase-2, tumor necrosis factor-α, and prostaglandin E2 synthases) increased markedly. Damages to the biofilm structure and inflammatory cell infiltration were simultaneously observed during histological examination. Heat-shock proteins were also expressed in large quantities after exposure to the poisons. Collectively, exposure to arsenite and/or Cu2+ can cause rectal damage in cockerels, inducing inflammation and an imbalance in immune system responses. Sometimes, exposure to both pollutants can produce even more toxic effects. Heat-shock proteins can protect the tissue from the exotoxins but the specific mechanisms require exploration. After oral ingestion of toxins, the rectum can still be damaged, necessitating attention to the safety of poultry breeding, human food safety, and environmental protection.
Asunto(s)
Trióxido de Arsénico/efectos adversos , Sulfato de Cobre/efectos adversos , Proteínas de Choque Térmico/metabolismo , Homeostasis/efectos de los fármacos , Inflamación/metabolismo , Recto/efectos de los fármacos , Animales , Trióxido de Arsénico/administración & dosificación , Pollos , Sulfato de Cobre/administración & dosificación , Suplementos Dietéticos/efectos adversos , Recto/metabolismoRESUMEN
This study was designed to evaluate the effects of zinc on inflammation and tight junction (TJ) in different intestinal regions of common carp under sub-chronic arsenic insult. Fish were exposed to zinc (0, 1â¯mg/L) and arsenic trioxide (0, 2.83â¯mg/L) in individual or combination for a month. Inflammatory infiltration and TJ structure changes were displayed by H&E staining and transmission electron microscope. To further explore these changes, biochemical indicator (SOD), gene or protein expressions of inflammatory responses (NF-κB, IL-1ß, IL-6 and IL-8) and TJ proteins (Occludin, Claudins and ZOs) were determined. In the anterior intestine, arsenic decreased activity of SOD, mRNA levels of Occludin, Claudins and ZOs, increased mRNA levels of ILs. However, unlike the anterior intestine, arsenic has an upregulation effects of Occludin and Claudin-4 in the mid intestine. These anomalies induced by arsenic, except IL-8, were completely or partially recovered by zinc co-administration. Furthermore, transcription factor (NF-κB) nuclear translocation paralleled with its downstream genes in both intestinal regions. In conclusion, our results unambiguously suggested that under arsenic stress, zinc can partly relieve intestinal inflammation and disruption of tight junction segment-dependently.
Asunto(s)
Arsénico/efectos adversos , Carpas , Enterotoxinas/efectos adversos , Enfermedades de los Peces/prevención & control , Intestinos/efectos de los fármacos , Sustancias Protectoras/farmacología , Zinc/farmacología , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Suplementos Dietéticos/análisis , Enfermedades de los Peces/inducido químicamente , Inflamación/inducido químicamente , Inflamación/prevención & control , Inflamación/veterinaria , Intestinos/fisiología , Uniones Estrechas/efectos de los fármacos , Uniones Estrechas/fisiologíaRESUMEN
Waterborne exposure to arsenic trioxide (As2O3) is inevitable due to its widespread industrial and agricultural applications. Oxidative stress and cascaded programmed cell death is now hypothesized to be the dominant mechanisms of arseniasis evidenced in vivo and in vitro. This study aimed to explore the interaction of divalent zinc ion (Zn2+), an efficient reactive oxygen species (ROS) scavenger with arsenite in the heart of common carp, and extensively investigated the exact signaling molecules involved. Significant induction of cardiotoxicity including oxidative stress, apoptosis and autophagy was evident in heart tissues following arsenite exposure (Pâ¯<â¯0.05). The dissipation of antioxidant enzymes (SOD and CAT) was induced by ROS burst, leading to oxidative damage and lipid peroxidation (MDA). Arsenite induced classic apoptotic hallmarks, characterized by chromatin degradation and subsequent formation of clumps adjacent, and elevated expression of Bax/Bcl-2 and Caspase family, and also increased autophagic flux evidenced by accelerated formation (LC3) and degradation (p62) of autophagosomes. PI3K/Akt/mTOR pathway was phosphorylated inhibited, while MAPK signaling (p38, ERK and JNK) displayed elevated phosphorylation levels in arsenite-exposed heart tissues. In contrast, above phenomena were effectively inhibited by Zn2+, which supplement attenuated arsenite-induced myocardial toxicity through inhibition of apoptosis and autophagy via PI3K/Akt/mTOR pathway, as well as suppressing intracellular ROS cluster via activating antioxidative system via MAPK pathway. Our results provided experimental explanation and evidences for cardiotoxicity of arsenite. Furthermore, our findings hint that the application of zinc preparations may provide a candidate for the prevention and treatment for arsenic poisoning.
Asunto(s)
Arsénico/toxicidad , Carpas/fisiología , Contaminantes Químicos del Agua/toxicidad , Animales , Antioxidantes/metabolismo , Apoptosis/efectos de los fármacos , Trióxido de Arsénico , Arsenitos , Autofagia/efectos de los fármacos , Cardiotoxicidad , Carpas/metabolismo , Suplementos Dietéticos , Estrés Oxidativo/efectos de los fármacos , Fosfatidilinositol 3-Quinasas/metabolismo , Fosforilación , Especies Reactivas de Oxígeno/metabolismo , Transducción de Señal/efectos de los fármacos , Zinc/químicaRESUMEN
The aim of this study is to investigate whether copper (Cu) could induce testicular poisoning and influence the mitochondrial dynamics, apoptosis, and autophagy in chickens. For this purpose, thirty-six 1-day-old male Hy-line chickens were divided into control group (C group) and test group (Cu group). The chickens were exposed to 0 (C group) or 300 mg/kg (Cu group) of copper sulfate (CuSO4) for 30, 60, and 90 days. CuSO4 was added into the basal diet to make supplements. Testis tissues were subjected to observation of ultrastructure and detection of testis-related indexes. The results indicated that in the test group, the levels of the pro-apoptotic genes were up-regulated and the levels of the anti-apoptotic genes were down-regulated; the levels of mitochondrial fission-related genes markedly increased, and the levels of mitochondrial fusion-related genes were highly decreased; autophagy-related gene (autophagy-associated gene 4B (ATG4B), dynein, microtubule-associated protein 1 light chain 3 beta (LC3-II), ATG5, and beclin-1) levels were increased, while mammalian target of rapamycin (mTOR) and LC3-I levels were declined. The results of transmission electron microscopy (TEM) demonstrated that Cu induced mitochondrial fragmentation, which induced autophagy and apoptosis in chicken testes. In conclusion, CuSO4 exposure can influence the mitochondrial dynamics balance and lead to mitochondria-initiated intrinsic pathway of apoptosis and autophagy, which triggers the testicular poisoning in chickens. What is more, there is a correlation among mitochondrial dynamics, apoptosis, and autophagy.
Asunto(s)
Apoptosis/efectos de los fármacos , Autofagia/efectos de los fármacos , Pollos/crecimiento & desarrollo , Cobre/toxicidad , Dinámicas Mitocondriales/efectos de los fármacos , Testículo/efectos de los fármacos , Animales , Apoptosis/genética , Proteínas Reguladoras de la Apoptosis/genética , Autofagia/genética , Pollos/genética , Expresión Génica/efectos de los fármacos , Masculino , Dinámicas Mitocondriales/genética , Proteínas Mitocondriales/genética , Testículo/ultraestructuraRESUMEN
Arsenic-based drugs as food additive were used in poultry. However, excessive arsenic exposure can disturb myocardial cell metabolism, which results in the inhibition of growth and development of chickens. Since disordered mitochondria influences cardiac physiology and pathology, a better understanding of the mechanisms modulating cardiomyocyte mitochondria process is critical for identifying the potent detoxication targets under arsenic exposure in chickens. Male Hy-line chickens (1-day-old) were fed either a basal diet or an arsenic trioxide (As2O3)-supplemented diet containing 7.5, 15, and 30 mg/kg As2O3 for 90 d. The concentrations of ions ([Na, Mg, Al, Si, K, Ca, As, Mn, Fe, Zn] and [Cr, Ni, Cu, Ba]) significantly increased and decreased in the heart of chicken under As2O3 exposure, respectively. Moreover, we observed that As2O3 decreased high-density lipoprotein cholesterol concentrations and increased total cholesterol concentrations in the serum. We also observed arterial wall degeneration, biochemical character of mitochondria undergoing either fission or fusion, typical apoptotic cells, typical DNA fragments and TdT-mediated dUTP nick end labeling positive nuclei under As2O3 exposure in the heart. Further quantitative real-time PCR demonstrated that B cell lymphoma/leukemia 2 (Bcl2) were significantly decreased and dynamin-related protein 1 (Drp1), Optic atrophy 1 (Opa1), mitochondrial fission factor 1 (Mfn1), Mfn2, p53, caspase-8, Bcl-2 associated X protein (Bax), caspase-3, caspase-9 and cytochrome C were significantly increased in all As2O3 group. In conclusion, As2O3 can disturb the trace elements homeostasis, which might favor the development of mitochondrial damage. Moreover, we suspected that As2O3-increased mitochondrial dynamics might trigger the apoptosis to limit cell metabolism. These features might identify the role of the mitochondrial dynamics under arsenic-induced cardiovascular disease in the chickens.
Asunto(s)
Trióxido de Arsénico/toxicidad , Arsénico/toxicidad , Cardiotoxicidad/metabolismo , Pollos/fisiología , Mitocondrias/metabolismo , Oligoelementos/metabolismo , Animales , Apoptosis/efectos de los fármacos , Cardiotoxicidad/etiología , Masculino , Mitocondrias/efectos de los fármacos , Distribución Aleatoria , Oligoelementos/deficienciaRESUMEN
Copper (Cu) is an essential trace element for organism of function properly. Overexposure to Cu causes chronic cardiac impairment. The aim of this study was to investigate the change of 28-trace element, inflammatory response, the possible mitochondrial dynamics and apoptosis under Cu exposure in the heart of chickens. Cupric sulfate (CuSO4) (300 mg/kg) was administered in a basal diet to male Hy-line chickens (one-day-old) for 90 days. Results showed the concentrations of Cu in the Cu group were increased by 57.8%, 27.57% and 57.2% at 30, 60 and 90 days, respectively. The Cu supplement caused trace elements imbalance, including reduced concentrations of B, Al, Ni, Ba, Pb and increased Li, Na, Mg, Si, K, Ca, V, Mn, Fe, Co, Zn, As, Mo in the heart of chickens. Exposure to Cu induced the TUNEL positive nuclei, histopathological alterations and ultrastructural apoptotic features. Moreover, Cu exposure activated the NF-κB-mediated pro-inflammatory cytokines, decreased the mRNA levels of opa1, mfn1, mfn2, Bcl-2, increased the mRNA levels of drp1, Bax, caspase-3, caspase-9, P53, while not altered Fas and caspase-8 compared with the control group. Similarly, western blot results showed the same trend of mRNA. Correlation analysis indicated that mitochondrial fission and intrinsic apoptosis might function synergistic. Moreover, mitochondrial network seem to function as cytosolic sensors for the induction of NF-κB mediated inflammatory responses. In summary, we speculated that Cu-induced redistribution of trace elements contributed to inflammatory response and disrupted the mitochondrial network via fission and intrinsic apoptosis in the heart of chickens.
Asunto(s)
Pollos/fisiología , Cobre/toxicidad , Oligoelementos/metabolismo , Animales , Apoptosis , Caspasa 3 , Caspasa 9 , Dieta , Masculino , Metales Pesados , Dinámicas Mitocondriales , FN-kappa B , Oligoelementos/análisisRESUMEN
To evaluate the toxicity of arsenic trioxide (As2O3) in the muscular tissues (wing, thigh and pectoral) of birds, 72 one-day-old Hy-line cocks were selected and randomly divided into four groups. They were fed either a commercial diet or an arsenic-supplemented diet containing 7.5, 15 or 30 mg/kg As2O3. The experiment lasted for 90 days and the samples of muscular tissues were collected at 30, 60 and 90 days. The results showed that As2O3 exposure significantly lowered the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GSH-Px)) and inhibition ability of hydroxyl radicals (OH) and increased the malondialdehyde (MDA) contents. Furthermore, the mRNA levels of inflammatory cytokines (tumor necrosis factor-α (TNF-α), nuclear factor-kappa B (NF-κB), cyclooxygenase-2 (COX-2), inducible NO synthase (iNOS), prostaglandin E synthase (PTGEs)) and heat shock proteins (HSPs) in muscular tissue were significantly upregulated in the As2O3 exposure groups. The results indicated that As2O3 exposure resulted in oxidative damage, induced the inflammatory response, and influenced the mRNA levels of HSPs in muscular tissue of cocks. Additionally, the results suggested that HSPs possibly resisted due to the As2O3 exposure-induced oxidative stress and inflammatory response, which provided a favorable environment and played protective roles in the muscular tissues of cocks. The information presented in this study is helpful to understand the mechanism of As2O3 toxicity in bird muscular tissues.
Asunto(s)
Pollos/fisiología , Sustancias Peligrosas/toxicidad , Proteínas de Choque Térmico/metabolismo , Músculos/efectos de los fármacos , Estrés Oxidativo/fisiología , Óxidos/toxicidad , Animales , Trióxido de Arsénico , Arsenicales , Biomarcadores/metabolismo , Catalasa/metabolismo , Citocinas/metabolismo , Glutatión Peroxidasa/metabolismo , Malondialdehído/metabolismo , Músculos/metabolismo , FN-kappa BRESUMEN
The contents of 28 trace elements, 17 amino acid were evaluated in muscular tissues (wings, crureus and pectoralis) of chickens in response to arsenic trioxide (As2O3). A total of 200 one-day-old male Hy-line chickens were fed either a commercial diet (C-group) or an As2O3 supplement diet containing 7.5mg/kg (L-group), 15mg/kg (M-group) or 30mg/kg (H-group) As2O3 for 90 days. The elements content was analyzed by inductively coupled plasma mass spectrometry (ICP-MS). Under As2O3 exposure, the concentration of As were elevated 8.87-15.76 fold, 7.93-15.63 fold and 5.94-12.45 fold in wings, crureus and pectoralis compared to the corresponding C-group, respectively. 19 element levels (lithium (Li), magnesium (Mg), aluminum (Al), silicon (Si), kalium (K), vanadium (V), chromium (Cr), manganese (Mn), nickel (Ni), copper (Cu), selenium (Se), strontium (Sr), molybdenum (Mo), cadmium (Cd), tin (Sn), antimony (Sb), barium (Ba), mercury (Hg) and lead (Pb), 9 element levels (K, Co, Ni, Cu, As, Se, Sr, Sn, Ba and Hg) and 4 element levels (Mn, cobalt (Co), As, Sr and Ba) were significantly increased (P < 0.05) in wing, crureus and pectoralis, respectively. 2 element levels (sodium (Na) and zinc (Zn)), 5 element levels (Li, Na, Si, titanium (Ti and Cr), 13 element levels (Li, Na, Mg, K, V, Cr, iron (Fe), Cu, Zn, Mo, Sn, Hg and Pb) were significantly decreased (P < 0.05) in wing muscle, crureus and pectoralis, respectively. Additionally, in crureus and pectoralis, the content of total amino acids (TAA) was no significant alterations in L and M-group and then increased approximately 10.2% and 7.6% in H-group, respectively (P < 0.05). In wings, the level of total amino acids increased approximately 10% in L-group, whereas it showed unchanged in M and H-group compared to the corresponding C-group. We also observed that significantly increased levels of proline, cysteine, aspartic acid, methionine along with decrease in the tyrosine levels in muscular tissues compared to the corresponding C-group. In conclusion, the residual of As in the muscular tissues of chickens were dose-dependent and disrupts trace element homeostasis, amino acids level in muscular tissues of chickens under As2O3 exposure. Additionally, the response (trace elements and amino acids) were different in wing, thigh and pectoral of chick under As2O3 exposure. This study provided references for further study of heavy metal poisoning and may be helpful to understanding the toxicological mechanism of As2O3 exposure in muscular tissues of chickens.
Asunto(s)
Aminoácidos/análisis , Alimentación Animal/análisis , Pollos/metabolismo , Músculos/metabolismo , Óxidos/toxicidad , Oligoelementos/análisis , Aminoácidos/metabolismo , Alimentación Animal/toxicidad , Animales , Trióxido de Arsénico , Arsenicales , Suplementos Dietéticos/análisis , Relación Dosis-Respuesta a Droga , Masculino , Músculos/química , Análisis Espectral , Oligoelementos/metabolismoRESUMEN
In many organ dysfunctions, arsenic and its compounds are well known to induce apoptosis by the mitochondria and death receptor apoptotic pathways in liver and airway. However, it is less reported that which signaling pathways contribute to excessive apoptosis of chicken immune organs, a major target of toxic metals biotransformation, which suffer from subchronic arsenism. In this study, we investigated whether the mitochondria or death receptor apoptotic pathways activated in the immune organs (spleen, thymus and bursa of Fabricius) of one-day-old male Hy-line chickens exposed to arsenic trioxide (As2O3), which were fed on diets supplemented with 0, 0.625, 1.25 and 2.5 mg/kg BW of As2O3 for 30, 60 and 90 days. We found that (1) Oxidative damage and inflammatory response were confirmed in the immune organs of chickens fed on As2O3 diet. (2) Subchronic arsenism induced typical apoptotic changes in ultrastructure. (3) TdT-mediated dUTP Nick-End Labeling (TUNEL) showed that the number of apoptotic cells significantly increased under subchronic arsenism. (4) As2O3-induced apoptosis of immune organs involved in mitochondrial pathway (decrease of B-cell lymphoma-2 (Bcl-2) and increase of protein 53 (p53), Bcl-2 Associated X Protein (Bax), caspase-9, caspase-3) and death receptor pathway (increase of factor associated suicide (Fas) and caspase-8). In conclusion, this work is the first to demonstrate that the activation of mitochondria and death receptor apoptosis pathways can lead to excessive apoptosis in immune organs of chickens, which suffer from subchronic arsenism, meanwhile, oxidative stress as well as subsequent inflammatory is a crucial driver of apoptosis.
Asunto(s)
Apoptosis/efectos de los fármacos , Intoxicación por Arsénico/patología , Mitocondrias/metabolismo , Estrés Oxidativo/efectos de los fármacos , Óxidos/envenenamiento , Receptores de Muerte Celular/metabolismo , Animales , Apoptosis/fisiología , Trióxido de Arsénico , Arsenicales , Bolsa de Fabricio/patología , Caspasa 3/metabolismo , Caspasa 8/metabolismo , Caspasa 9/metabolismo , Pollos , Etiquetado Corte-Fin in Situ , Inflamación/inducido químicamente , Masculino , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Bazo/patología , Timo/patología , Proteína X Asociada a bcl-2/metabolismoRESUMEN
The aim of this study was to assess the effects of arsenic trioxide (As2O3) in the chicken heart, and 72 1-day-old male Hy-line chickens were fed either a commercial diet (C group) or an arsenic supplement diet containing 7.5 mg/kg (L group), 15 mg/kg (M group), or 30 mg/kg (H group) As2O3 for 90 days. The results showed that exposure to As2O3 merely lowered (P < 0.05) the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) in M and H groups at 90 days, significantly downregulated the inhibition ability of hydroxyl radicals (OH·), and upregulated (P < 0.05) the contents of malondialdehyde (MDA) in As2O3 exposure groups at 30, 60, and 90 days. Meanwhile, the messenger RNA levels of inflammatory cytokines (tumor necrosis factor-α (TNF-α), nuclear factor-kappa B (NF-κB), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and prostaglandin E synthase (PTGEs)) significantly increased (P < 0.05) in As2O3 exposure groups at 30, 60, and 90 days, and histological and ultrastructural damage was observed in As2O3 exposure groups. Additionally, As2O3-induced cardiac enzyme (aspartate transaminase (AST), creatine kinase (CK), creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and α-hydroxybutyrate dehydrogenase (α-HBDH)) levels increased (P < 0.05) at 90 days. These findings suggested that As2O3 exposure led to oxidative stress, inflammatory response, and histological and ultrastructural damage and altered the levels of cardiac enzymes in chicken heart tissues. This result may be helpful for further studies on the toxicological mechanisms of As2O3 in the chicken heart.
Asunto(s)
Pollos , Corazón , Óxidos/toxicidad , Animales , Arsénico , Trióxido de Arsénico , Arsenicales , Catalasa , Citocinas , Glutatión Peroxidasa , Hidroxibutirato Deshidrogenasa , Masculino , Malondialdehído , FN-kappa B , Óxido Nítrico Sintasa de Tipo II , Estrés Oxidativo , Factor de Necrosis Tumoral alfaRESUMEN
This study assessed the impacts of dietary arsenic trioxide (As2O3) on 26 mineral element contents in the liver and kidney of chicken. A total of 100 male Hy-line cocks were randomly divided into 2 groups (50 chickens in each group), including an arsenic-treated group (basic diet supplemented with As2O3 at 30 mg/kg) and a control group (basal diet). The feeding experiment lasted for 90 days and the experimental animals were given free access to feed and water. We determined 26 mineral elements in the liver and kidney by inductively coupled plasma mass spectrometry (ICP-MS). The results showed that nine element levels (Al, Mn, Co, Cu, Zn, Se, Cd, Ba, and Pb) were significantly decreased (P < 0.05) in the liver of chickens exposed to As2O3 compared to the control chickens where three element levels (Ni, As, and Hg) increased significantly (P < 0.05). The results in the kidney showed that nine element levels (Al, K, Ca, Cr, Mn, Ni, Sb, Ba, and Pb) were significantly decreased (P < 0.05) in the chickens exposed to As2O3 compared to the control chickens where four element levels (Mo, As, Cd, and Hg) increased significantly (P < 0.05). These results suggest that supplementation of high levels of arsenic affected trace mineral levels in the liver and kidney of chicken, and the effects vary from organ to organ. The aim of this study is to provide references for further study of heavy metal poisoning by detecting the contents of minerals induced by arsenic in chicken.
Asunto(s)
Alimentación Animal , Arsenicales , Espectrometría de Masas/métodos , Óxidos , Alimentación Animal/análisis , Alimentación Animal/toxicidad , Animales , Trióxido de Arsénico , Arsenicales/análisis , Pollos , Riñón/química , Riñón/efectos de los fármacos , Riñón/metabolismo , Hígado/química , Hígado/efectos de los fármacos , Hígado/metabolismo , Óxidos/análisis , Óxidos/toxicidad , Distribución AleatoriaRESUMEN
OBJECTIVE: To investigate systematically the application of functional ingredients among healthy food products during 1996 to 2007, which existed from the 10 years periods by government approved, to assess the character or condition of ingredients, and analyze the reasons of it's centralization and contribution. METHODS: Data of functional/bioactive ingredients in functional/healthy food approved by government during 1996 to 2007 were recorded, and selected qualified products to built nutritional and functional ingredients database. Then, The profile map and contribution of each classified of the data was analyzed by statistics method and computer software. RESULTS: Total 9021 products were approved by gov. during 1996 to 2007, according the condition of samples, qualified 8645 products was as studied samples. The research had showed that Chinese herbs was the main port in ingredients, total 223-378 herbs were used. Nutritional and functional ingredients were second main ingredients, it was high numbers (280 more) among 8645 products. Flavonoids, Saponins and Polysaccharide are applied most widely, which is often to apply as the representing or symbolic substance when the product used a herb material as ingredient. CONCLUSION: Functional ingredients have a variegated appearance along with existent 27 function claims within regulation system. A representing substance was used as a feature that was a universality labeled to declare the characteristics or qualities of herb products. This should be individually reviewed in further and increase the technique on herb and claim.
Asunto(s)
Suplementos Dietéticos , Medicamentos Herbarios Chinos/química , Alimentos Orgánicos/normas , Alimentos Funcionales/normas , China , Seguridad de Productos para el Consumidor , Etiquetado de Alimentos/legislación & jurisprudencia , Humanos , Legislación AlimentariaRESUMEN
OBJECTIVE: To study the effect on the retention factors (RFs) of vitamin and mineral in potato cooked by different methods, to provide reference for evaluation of diet's nutrition and the RF decision of similar vegetables in our country. METHODS: Potato were selected and cooked by different methods, such as frying, braising, boiling deep frying and steaming, according to local tradition and custom. Meanwhile, weights of potato before and after cooking were recorded. Respectively vitamin and mineral contents in every sample were analyzed with the national standard methods. RESULTS: The RF value of vitamin C was higher during braising than that of during frying, boiling, deep frying and steaming. The RF values of thiamin, riboflavin, vitamin B6 and niacin were more loss during boiling and deep frying than those during other methods. The RFs of every minerals were more loss only during boiling. CONCLUSION: Cooking may change the contents of vitamins and minerals in potato. There could be for one nutrient different RFs during different cooking methods.