[Studies on characteristic fingerprints of fat-soluble components of Marsdenia tenacissima by GC].

OBJECTIVE: To study characteristic fingerprints of the fat-soluble components of different Marsdenia tenacissima samples by GC and GC-MS. METHOD: The sample was split in the 280 degrees C injection port, with 10:1 split ratio, and separated on a fused silica capillary column (DB-5, 30 m x 0.25 mm I. D., 0.25 microm film). The temperature program was 70 degrees C for 5 min, 8 degrees C x min(-1) to 200 degrees C, then 5 degrees C x min-1 to 260 degrees C, 260 degrees C for 10 min. The high pure nitrogen was used as a carrier gas, The FID temperature was 300 degrees C. RESULT: There are 15 common peaks in the GC fingerprints of M. tenacissima and they are identified by GC-MS. The analyses on cluster and similarity degree of the finger the crude drugs from various habitats have been performed. CONCLUSION: The method has good repeatability. The GC fingerprint combined with the HPLC fingerprint of M. tenacissim can be used to evaluate its quality integrally.

Analysis of Psoralea corylifolia L. fruits in different regions.

Application of multivariate data analysis has become a popular method in the last decades, mainly because it can provide information not otherwise accessible. The information includes classification, searching similarities, finding relationships, finding physical significance to principal components, etc. Twenty-two Chinese medicinal herbs containing twelve constituents were collected and determined by HPLC. The results were studied by hierarchical cluster analysis (HCA) and principal components analysis (PCA). It was shown that the samples could be clustered reasonably into three groups, hence corresponding with the typical habitats of Psoralea corylifolia L.

Determination of five coumarins in Cnidii Fructus by micellar electrokinetic capillary chromatography.

A micellar electrokinetic capillary chromatography (MEKC) method for the separation and quantification of the coumarins in Cnidii Fructus was developed for the first time. Within 25 minutes, 5 major coumarins were separated using 18 mM borate, 12 mM phosphate and 50 mM SDS (pH 9.2) containing 20 % methanol. The relative standard deviations of migration times and peak areas were less than 5 %. The total contents of the five coumarins in plants from different habitats ranged from 13.6 to 44.6 mg/g, and recoveries ranged from 96.0 to 102.8 %. The effects of buffer concentration, SDS, pH value and organic modifier on the separation were investigated.