RESUMEN
PURPOSE: In this study, we elucidated the effects of berberine, a major alkaloid component contained in medicinal herbs, such as Phellodendri Cortex and Coptidis Rhizoma, on expression of monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) in a human retinal pigment epithelial cell line (ARPE-19) caused by lipopolysaccharide (LPS) stimulation. METHODS: ARPE-19 cells were cultured to confluence. Berberine and LPS were added to the medium. MCP-1 and IL-8 mRNA were measured by real-time polymerase chain reaction. MCP-1 and IL-8 protein concentrations in the media were measured using enzyme-linked immunosorbent assay. RESULTS: After stimulation with LPS, MCP-1 and IL-8 mRNA in ARPE-19 cells reached maximum levels at 3 h, and MCP-1 and IL-8 protein in the culture media reached maximum levels at 24 h. Berberine dose-dependently inhibited MCP-1 and IL-8 mRNA expression of the cells and protein levels in the media stimulated with LPS. CONCLUSIONS: These findings indicate that berberine inhibited the expression of MCP-1 and IL-8 induced by LPS.
Asunto(s)
Berberina/farmacología , Quimiocina CCL2/genética , Regulación de la Expresión Génica/efectos de los fármacos , Interleucina-8/genética , Degeneración Macular/genética , Epitelio Pigmentado Ocular/metabolismo , ARN/genética , Células Cultivadas , Quimiocina CCL2/biosíntesis , Ensayo de Inmunoadsorción Enzimática , Humanos , Interleucina-8/biosíntesis , Lipopolisacáridos/farmacología , Degeneración Macular/metabolismo , Degeneración Macular/patología , Epitelio Pigmentado Ocular/efectos de los fármacos , Epitelio Pigmentado Ocular/patologíaRESUMEN
PURPOSE: To examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on the disruption of the barrier function in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin-1beta (IL-1beta). METHODS: ARPE-19 cells were cultured to confluence. Berberine and IL-1beta were added to the medium. Barrier functions were evaluated by measuring transepithelial electrical resistance (TER) and the permeability to horseradish peroxidase (HRP) and sodium fluorescein (SF). RESULTS: Berberine dose-dependently inhibited decreased TER and increased the permeability to HRP and SF in the cells stimulated with IL-1beta. CONCLUSIONS: Berberine dose-dependently inhibited the disruption of the barrier function in the ARPE-19 cell line induced by IL-1beta.
Asunto(s)
Berberina/farmacología , Barrera Hematorretinal/fisiología , Epitelio Pigmentado Ocular/efectos de los fármacos , Transporte Biológico/efectos de los fármacos , Línea Celular , Permeabilidad de la Membrana Celular , Relación Dosis-Respuesta a Droga , Impedancia Eléctrica , Electrofisiología , Fluoresceína/metabolismo , Peroxidasa de Rábano Silvestre/metabolismo , Humanos , Interleucina-1beta/farmacología , Epitelio Pigmentado Ocular/metabolismoRESUMEN
PURPOSE: The aims of this study were to examine the in vivo effects of berberine, an alkaloid isolated from some medicinal herbs, on monocyte chemotactic protein-1 (MCP-1) and cytokine-induced neutrophil chemoattractant-1 (CINC-1) expression in rat lipopolysaccharide (LPS)-induced uveitis. METHODS: LPS was injected intraperitoneally. Berberine was orally administered. MCP-1 mRNA and CINC-1 mRNA were measured by semiquantitative reverse-transcription polymerase chain reaction and real-time polymerase chain reaction. MCP-1 and CINC-1 protein concentration in the aqueous humor were measured by enzyme-linked immunosorbent assay. Histopathologic study was performed in the anterior ocular segments. RESULTS: Berberine dose-dependently inhibited LPS-induced MCP-1 mRNA and CINC-1 mRNA expression of the iris-ciliary body. The alkaloid inhibited chemokines, protein and cell levels in the aqueous humor in rats stimulated with LPS. On histopathologic study, the inflammatory cell infiltration was diminished by the berberine treatment. CONCLUSIONS: These findings indicate that berberine dose-dependently inhibited the expression of MCP-1 and CINC-1 induced by LPS and diminished the anterior uveitis.
Asunto(s)
Berberina/uso terapéutico , Quimiocina CCL2/genética , Quimiocinas CXC/genética , Expresión Génica/efectos de los fármacos , ARN Mensajero/metabolismo , Uveítis Anterior/tratamiento farmacológico , Animales , Quimiocina CXCL1 , Cuerpo Ciliar/metabolismo , Modelos Animales de Enfermedad , Ensayo de Inmunoadsorción Enzimática , Iris/metabolismo , Lipopolisacáridos/toxicidad , Masculino , Reacción en Cadena de la Polimerasa , Ratas , Ratas Wistar , Uveítis Anterior/inducido químicamente , Uveítis Anterior/metabolismoRESUMEN
PURPOSE: The aims of this study were to examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on interleukin 8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) expression in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin 1beta (IL-1beta) or tumor necrosis factor alpha (TNF-alpha). METHODS: ARPE-19 cells were cultured to confluence. Berberine and IL-1beta or TNF-alpha were added to the medium. IL-8 mRNA and MCP-1 mRNA were measured by semiquantitative reverse-transcription polymerase chain reaction and real-time polymerase chain reaction. IL-8 and MCP-1 protein concentrations in the media were measured using enzyme-linked immunosorbent assay. RESULTS: Berberine dose-dependently inhibited IL-8 mRNA and MCP-1 mRNA expression of the cells and protein levels in the media stimulated with IL-1beta or TNF-alpha. CONCLUSION: These findings indicate that berberine dose-dependently inhibited the expression of IL-8 and MCP-1 induced by IL-1beta or TNF-alpha.