RESUMEN
Selenium (Se) is a vital microelement for spermatogenesis and male fertility. The aim of this study was to investigate the effects of Se on the male reproductive function and possible mechanisms. Fourty male mice were randomly divided into 0, 0.1, 0.3 and 0.9 mg/kg Se supplementation groups and given with Se dietary intervention for 12 weeks. Our data showed that excessive Se intake damaged the tissue structure of testes and epididymides of the mice, resulting in decreased sperm quality and quantity. Moreover, excessive Se induced oxidative stress, causing DNA damage and activated DNA damage repair factors (Mre11/Rad50/Nbs1), and also disrupted telomere function by shortening telomere length and decreasing TERT expression. Se excess activated the senescence pathway p53/p21/p16, leading to germ cell senescence, and inhibited cell proliferation by suppressing the Sirt1/Foxo1/c-Myc pathway. All of this led to spermatogenic cell apoptosis, thereby causing a decrease of sperm quantity and quality. In conclusion, excessive Se caused reproductive toxicity via inducing telomere dysfunction due to DNA damage, leading to germ cellular senescence and apoptosis in the testes of male mice. Our research provide new proof to explain the underlying mechanism of male reproductive toxicity triggered by excessive Se intake.
Asunto(s)
Desnutrición , Selenio , Ratones , Masculino , Animales , Selenio/farmacología , Semen , Espermatogénesis , Senescencia Celular , Apoptosis , Daño del ADN , TelómeroRESUMEN
In recent years, decabromodiphenyl ethane (DBDPE), a new alternative flame retardant to the decabrominated diphenyl ethers (BDE-209), is widely used in a variety of products. Previous studies have indicated that DBDPE, like BDE-209, could disrupt thyroid function. However, compared with BDE-209, the degrees of thyrotoxicosis induced by DBDPE were not clear. In addition, the mechanism of thyrotoxicosis induced by DBDPE or BDE-209 was still under further investigation. In this study, male rats as a model were orally exposed to DBDPE or BDE-209 by 5, 50, 500â¯mg/kg bw/day for 28 days. Then, we assessed the thyrotoxicosis of DBDPE versus BDE-209 and explored the mechanisms of DBDPE and BDE-209-induced thyrotoxicosis. Results showed that decreased free triiodothyronine (FT3) and increased thyroid-stimulating hormone (TSH) and thyrotropin-releasing hormone (TRH) in serum were observed in both 500â¯mg/kg bw/day BDE-209 and DBDPE group. Decreased total thyroxine (TT4), total T3 (TT3), and free T4 (FT4) were only observed in BDE-209 group but not in DBDPE group. Histological examination and transmission electron microscope examination showed that high level exposure to BDE-209 and DBDPE both caused significant changes in histological structure and ultrastructure of the thyroid gland. Additionally, oxidative damages of thyroid gland (decreased SOD and GSH activities, and increased MDA content) were also observed in both BDE-209 and DBDPE groups. TG contents in the thyroid gland was reduced in BDE-209 group but not in DBDPE group. Both BDE-209 and DBDPE affected the expression of hypothalamic-pituitary-thyroid (HPT) axis related genes. These findings suggested that both BDE-209 and DBDPE exposure could disrupt thyroid function in the direction of hypothyroidism and the underlying mechanism was likely to be oxidative stress and perturbations of HPT axis. However, DBDPE was found to be less toxic than BDE-209.
Asunto(s)
Bromobencenos/toxicidad , Disruptores Endocrinos/toxicidad , Retardadores de Llama/toxicidad , Éteres Difenilos Halogenados/toxicidad , Glándula Tiroides/efectos de los fármacos , Animales , Relación Dosis-Respuesta a Droga , Hipotálamo/efectos de los fármacos , Hipotálamo/metabolismo , Hipotálamo/patología , Masculino , Estrés Oxidativo/efectos de los fármacos , Hipófisis/efectos de los fármacos , Hipófisis/metabolismo , Hipófisis/patología , Ratas , Ratas Sprague-Dawley , Glándula Tiroides/metabolismo , Glándula Tiroides/ultraestructura , Tirotropina/sangre , Hormona Liberadora de Tirotropina/sangre , Triyodotironina/sangreRESUMEN
Given that the effects of ultrafine fractions (< 0.1 µm) on reproductive diseases are gaining attention, this study aimed to explore the influence of silica nanoparticle (SiNP)-induced female reproductive dysfunction. In this study, 80 female mice were randomly divided into four groups including a control group and three concentrations of SiNP groups (7, 21, 35 mg/kg). Mice were exposed to the vehicle control and silica nanoparticles by tracheal perfusion every 3 days a total of five times in 15 days. Then, half of the mice in each group were sacrificed on 15 and 30 days after the first dose, respectively. Our findings indicated that SiNPs can result in ovarian damage, cause an imbalance of sex hormones, increase the number of atretic and primary follicles, and induce oxidative stress and DNA strand breaks in ovary by day 15. The protein expressions of ATM, CHK-2, P53, E2F1, P73, BAX, Caspase-9, and Caspase-3 were significantly increased, while expressions of RAD51 were down-regulated after SiNP exposure by days 15. Estradiol increased, while progesterone increased in low dose and decreased in high dose after SiNP exposure by 15 days. However, these changes were recovered by 30 days. The results suggest that SiNPs can cause reversible damage to follicles in mice. SiNPs could primarily cause DNA damage and DNA damage response through oxidative stress, while DNA damage repair failure because of severe DNA damage activated the mitochondrial apoptosis pathway and therefore resulted in apoptosis of granulosa cell. In addition, the disorder of reproductive endocrine function caused by SiNPs could be another reason for SiNP-induced reproductive dysfunction in mice. These events in turn induce the follicles to undergo atresia.
Asunto(s)
Apoptosis/efectos de los fármacos , Atresia Folicular/efectos de los fármacos , Células de la Granulosa/efectos de los fármacos , Nanopartículas/toxicidad , Dióxido de Silicio/toxicidad , Animales , Apoptosis/genética , Femenino , Regulación de la Expresión Génica/efectos de los fármacos , Hormonas Esteroides Gonadales/genética , Células de la Granulosa/patología , Ratones , Ratones Endogámicos BALB C , Nanopartículas/química , Ovario/efectos de los fármacos , Ovario/patología , Estrés Oxidativo/efectos de los fármacos , Dióxido de Silicio/químicaRESUMEN
Autophagy dysfunction is considered as a potential toxic mechanism of nanomaterials. Silica nanoparticles (SiNPs) can induce autophagy, but the specific mechanism involved remains unclear. Therefore, the aim of this study was to confirm the effects of SiNPs on autophagy dysfunction and explore the possible underlying mechanism. In this article, we reported that cell-internalized SiNPs exhibited dose- and time-dependent cytotoxicity in both L-02 and HepG2 cells. Multiple methods verified that SiNPs induced autophagy even at the noncytotoxic level and blocked the autophagic flux at the high-dose level. Notably, SiNPs impaired the lysosomal function through damaging lysosomal ultrastructures, increasing membrane permeability, and downregulating the expression of lysosomal proteases, cathepsin B, as evidenced by transmission electron microscopy, acridine orange staining, quantitative reverse transcription-polymerase chain reaction, and Western blot assays. Collectively, these data concluded that SiNPs inhibited autophagosome degradation via lysosomal impairment in hepatocytes, resulting in autophagy dysfunction. The current study not only discloses a potential mechanism of autophagy dysfunction induced by SiNPs but also provides novel evidence for the study of toxic effect and safety evaluation of SiNPs.
Asunto(s)
Autofagosomas/metabolismo , Autofagia/efectos de los fármacos , Hepatocitos/efectos de los fármacos , Lisosomas/patología , Nanopartículas/química , Dióxido de Silicio/química , Western Blotting , Células Cultivadas , Técnica del Anticuerpo Fluorescente , Células Hep G2 , Hepatocitos/citología , Hepatocitos/metabolismo , Humanos , Lisosomas/metabolismo , Microscopía Confocal , Microscopía Electrónica de Transmisión , Nanopartículas/administración & dosificaciónRESUMEN
Environmental exposure to silica nanoparticles (SiNPs) is inevitable due to their widespread application in industrial, commercial, and biomedical fields. In recent years, most investigators focus on the evaluation of cardiovascular effects of SiNPs in vivo and in vitro. Endothelial injury and dysfunction is now hypothesized to be a dominant mechanism in the development of cardiovascular diseases. This study aimed to explore interaction of SiNPs with endothelial cells, and extensively investigate the exact effects of reactive oxygen species (ROS) on the signaling molecules and cytotoxicity involved in SiNPs-induced endothelial injury. Significant induction of cytotoxicity as well as oxidative stress, apoptosis, and autophagy was observed in human umbilical vein endothelial cells following the SiNPs exposure (P<0.05). The oxidative stress was induced by ROS generation, leading to redox imbalance and lipid peroxidation. SiNPs induced mitochondrial dysfunction, characterized by membrane potential collapse, and elevated Bax and declined bcl-2 expression, ultimately leading to apoptosis, and also increased number of autophagosomes and autophagy marker proteins, such as LC3 and p62. Phosphorylated ERK, PI3K, Akt, and mTOR were significantly decreased, but phosphorylated JNK and p38 MAPK were increased in SiNPs-exposed endothelial cells. In contrast, all of these stimulation phenomena were effectively inhibited by N-acetylcysteine. The N-acetylcysteine supplement attenuated SiNPs-induced endothelial toxicity through inhibition of apoptosis and autophagy via MAPK/Bcl-2 and PI3K/Akt/mTOR signaling, as well as suppression of intracellular ROS property via activating antioxidant enzyme and Nrf2 signaling. In summary, the results demonstrated that SiNPs triggered autophagy and apoptosis via ROS-mediated MAPK/Bcl-2 and PI3K/Akt/mTOR signaling in endothelial cells, and subsequently disturbed the endothelial homeostasis and impaired endothelium. Our findings may provide experimental evidence and explanation for cardiovascular diseases triggered by SiNPs. Furthermore, results hint that the application of antioxidant may provide a novel way for safer use of nanomaterials.
Asunto(s)
Apoptosis/efectos de los fármacos , Biomarcadores/metabolismo , Células Endoteliales de la Vena Umbilical Humana/patología , Nanopartículas/administración & dosificación , Transducción de Señal/efectos de los fármacos , Dióxido de Silicio/química , Autofagia/efectos de los fármacos , Western Blotting , Células Cultivadas , Activación Enzimática , Células Endoteliales de la Vena Umbilical Humana/efectos de los fármacos , Células Endoteliales de la Vena Umbilical Humana/metabolismo , Humanos , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Proteína Quinasa 1 Activada por Mitógenos/genética , Proteína Quinasa 1 Activada por Mitógenos/metabolismo , Nanopartículas/química , Estrés Oxidativo/efectos de los fármacos , Fosfatidilinositol 3-Quinasas/genética , Fosfatidilinositol 3-Quinasas/metabolismo , Fosforilación/efectos de los fármacos , Proteínas Proto-Oncogénicas c-akt/genética , Proteínas Proto-Oncogénicas c-akt/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/genética , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Serina-Treonina Quinasas TOR/genética , Serina-Treonina Quinasas TOR/metabolismoRESUMEN
We determined the effect of dietary supplementation with vitamin E (0-, 50-, 250-, 500-, 1000- and 5000-mg/kg diet for 4 weeks) on antistress ability in juvenile soft-shelled turtle (Pelodiscus sinensis). Half of the turtles per dose group were treated by acid stress for 24 h. The results showed that phagocytosis of blood cells in the control group significantly decreased after acid stress while the other five groups had no significant changes compared with those of before stress. Serum bacteriolytic activity in the control group and the group supplemented with 50-mg vitamin E/kg diet significantly decreased after acid stress. The other four groups showed no significant differences compared with those before stress. Serum bactericidal activities in all groups notably decreased after acid stress, but the difference of serum bactericidal activity in before and after stress had a decreased tendency from the control group to the highest dose group. Serum cortisol levels in the control group were significantly increased while the other five groups had no notable increases after acid stress. Liver vitamin E levels in all groups had no notable changes compared with those before stress but there was a tendency to decrease after acid stress. These results suggest that acid stress depress immune function and increase serum cortisol levels in turtles while vitamin E alleviate the adverse effects caused by acid stress.
Asunto(s)
Ácidos/farmacología , Estrés Fisiológico/inducido químicamente , Estrés Fisiológico/fisiopatología , Tortugas/fisiología , Vitamina E/farmacología , Animales , Bacterias/inmunología , Suplementos Dietéticos , Hidrocortisona/sangre , Hígado/química , Hígado/efectos de los fármacos , Hígado/metabolismo , Fagocitosis/efectos de los fármacos , Estrés Fisiológico/sangre , Estrés Fisiológico/inmunología , Tortugas/sangre , Tortugas/inmunología , Tortugas/microbiología , Vitamina E/administración & dosificación , Vitamina E/análisis , Vitamina E/metabolismo , Agua/químicaRESUMEN
We studied the effect of dietary vitamin C on growth, liver vitamin C and serum cortisol levels in stressed and unstressed juvenile soft-shelled turtles. Turtles were fed with vitamin C supplementation at dosages of 0, 250, 500, 2500, 5,000 or 10,000 mg/kg diet for 4 weeks. Vitamin C supplementation exerted significant effects on specific growth rate and liver vitamin C concentrations. The specific growth rate peaked in the group fed at 500 mg/kg diet, while liver vitamin C levels increased with increasing dietary vitamin C levels. Serum cortisol concentrations did not differ between groups of turtles fed diets supplemented with vitamin C in the range of 0-10,000 mg/kg. After acid stress, hepatic vitamin C levels were unaffected, while serum cortisol in the control group was significantly elevated (P<0.01). The other five groups of turtles did not show significant changes in serum cortisol compared with pre-stress levels.
Asunto(s)
Ácido Ascórbico/farmacología , Hígado/metabolismo , Tortugas/fisiología , Animales , Ácido Ascórbico/administración & dosificación , Ácido Ascórbico/análisis , Dieta/veterinaria , Relación Dosis-Respuesta a Droga , Hidrocortisona/sangre , Hígado/efectos de los fármacos , Estrés Fisiológico/sangre , Estrés Fisiológico/inducido químicamente , Tortugas/sangre , Tortugas/crecimiento & desarrolloRESUMEN
The study was conducted to determine the effect of supplementation vitamin C in dietary non-specific immunity in juvenile soft-shelled turtles. The soft-shelled turtles were fed with vitamin C supplementation at dosages of 0, 250, 500, 2500, 5000 and 10000 mg/kg diets, respectively, for 4 weeks. The results showed that there were no differences in the phagocytosis of blood cells, serum bacteriolytic activity and bactericidal activity among soft-shelled turtles fed with vitamin C supplementation in 0-500 mg/kg diets. However, firm indicators were significantly enhanced in soft-shelled turtles fed with vitamin C supplementation at 2500 mg/kg diets compared with those fed at 0 and 250 mg/kg diets. The soft-shelled turtles fed with 5000 mg/kg diets had only significantly higher bactericidal activity than those fed vitamin C-deficient diets. The vitamin C supplementation in 10000 mg/kg diets had no notable effects on the phagocytosis, bacteriolytic activity and bactericidal activity. These results suggest that vitamin C seems have an upper and lower threshold for improving non-specific immune function, and the optimum dose was 2500 mg/kg.