RESUMEN
OBJECTIVE: To research the preparation technology and dissolution of Blumea volatile oil suppository. METHOD: In order to establish the content determination and methodology inspection method of Blumea volatile oil plug, the extraction process of Blumea volatile oil was optimized by using orthogonal test. Optimization on the investigation to the suppository matrix by melting time, appearance and dissolution was carried on. The best prescription craft was determined by determining the best molding temperature, dosage of the matrix and complementary makings. The determination method of dissolution was established by investigating different dissolution method and its impact on the preparation of dissolution. RESULT: The best conditions of steam distillation extracted Blumea volatile oil was as followed, the ratio of gardenia to liquor 1:6, 2.5% drug amount of sodium, 8 hours of extracting time. The optimum temperature for mold was 60-65 degrees C. Preparation technique of Blumea volatile oil suppository was stable, which after 45 minutes and 3 h in pH 4.5 PBS released at least 70% and 90%. CONCLUSION: Blumea volatile oil suppository with rational prescription, simple preparation and good stability.
Asunto(s)
Asteraceae/química , Química Farmacéutica/métodos , Medicamentos Herbarios Chinos/química , Aceites Volátiles/química , Aceites de Plantas/química , Destilación , Medicamentos Herbarios Chinos/aislamiento & purificación , Aceites Volátiles/aislamiento & purificación , Aceites de Plantas/aislamiento & purificación , Solubilidad , TemperaturaRESUMEN
An on-line high performance liquid chromatography (HPLC)-diode array detector (DAD)-electrospray ionization mass spectrometry (ESI-MS) method has been developed to quantify simultaneously eight bioactive chemical components in Houttuynia cordata Thunb and related Saururaceae medicinal plants. Simultaneous separation of these eight compounds was achieved on a C(18) analytical column with gradient elution of acetonitrile and 0.2% acetic acid (v/v) at a flow rate of 0.6 mL/min and being detected at 280 nm. These eight compounds were completely separated within 90 min. Good linear regression relationship (r(2)>0.9978) within test ranges was shown in all calibration curves. Good repeatabilty for the quantification of these eight compounds in H.cordata was also demonstrated in this method, with intra- and inter-day variations less than 3.0%. The method established was successfully applied to quantify eight bioactive compounds in closely related species of H.cordata, which provides a new basis for quality assessment of H.cordata.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Medicamentos Herbarios Chinos/química , Houttuynia/química , Saururaceae/química , Espectrometría de Masa por Ionización de Electrospray/métodos , Medicamentos Herbarios Chinos/aislamiento & purificación , Modelos Lineales , Plantas Medicinales/química , Control de Calidad , Reproducibilidad de los ResultadosRESUMEN
OBJECTIVE: To study the aqueous constituents of Houttuynia cordata. METHOD: Various columns including Diaion HP-20, Sephadex LH-20, ODS and silica gel were employed for the isolation and purification of compounds from H. cordata. The structures of the compounds were identified by physiochemical properties and spectral analysis. RESULT: Five compounds were isolated, and their structures were identified as chlorogenic methyl ester (1), (E)-4-Hydroxy-4-[3'-(beta-D-glucopyranosyloxy) butylidene]-3, 5, 5-trimethyl-2-cyclohexen-1-one (2), 2-(3, 4-dihydroxyphenyl) ethyl-beta-D-glucopyranoside (3), p-hydroxyphenethyl-beta-D-glucoside (4), 4-(beta-D-glucopyranosyloxy)-3-hydroxy-Benzoic acid (5). CONCLUSION: All compounds were isolated from this plant for the first time.
Asunto(s)
Ácido Clorogénico/análogos & derivados , Ciclohexanonas/aislamiento & purificación , Glucósidos/aislamiento & purificación , Houttuynia/química , Plantas Medicinales/química , Ácido Clorogénico/química , Ácido Clorogénico/aislamiento & purificación , Cromatografía en Gel , Ciclohexanonas/química , Glucósidos/química , Espectroscopía de Resonancia Magnética , Fenoles/química , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
OBJECTIVE: To optimize the extraction procedure of essential oil from H. cordata using the SFE-CO2 and analyze the chemical composition of the essential oil. METHOD: The extraction procedure of essential oil from fresh H. cordata was optimized with the orthogonal experiment. Essential oil of fresh H. cordata was analysed by GC-MS. RESULT: The optimize preparative procedure was as follow: essential oil of H. cordata was extracted at a temperature of 35 degrees C, pressure of 15,000 kPa for 20 min. 38 chemical components were identified and the relative contents were quantified. CONCLUSION: The optimum preparative procedure is reliable and can guarantee the quality of essential oil.