RESUMEN
OBJECTIVE: To establish the analytical method for the fingerprint of Scutellariae Radix by MEKC-DAD and compare the fingerprints of Scutellariae Radix, Scutellariae Radix Stir-baked and Scutellariae Radix Green. METHODS: Based on the mode of micellar electrokinetic chromatography, 40 mmol/L sodium hydrogen phosphate, 15 mmol/L sodium borate, 40 mmol/L SDS, 15% acetonitrile, 7.5% propyl alcohol were selected for the running buffer (pH 8.4). The separation voltage was 20 kV and the detection wavelength was set at 280 nm. Baicalin was used as reference standard, the chromatographic fingerprint was established. RESULTS: MEKC-DAD fingerprint with 9 main peaks was established preliminarily. Regarding to the fingerprints of Scutellariae Radix and its processed products, the samples before and after storage moisture, there were obvious differences in the relative areas of common peaks. CONCLUSION: The method is reliable, accurate and can be used for quality control of Scutellariae Radix.
Asunto(s)
Cromatografía Capilar Electrocinética Micelar/métodos , Medicamentos Herbarios Chinos/química , Scutellaria baicalensis/química , Boratos/química , Tampones (Química) , Medicamentos Herbarios Chinos/normas , Raíces de Plantas/química , Control de Calidad , Reproducibilidad de los ResultadosRESUMEN
OBJECTIVE: To establish the analytical method for the fingerprint of Salviae Miltiorrhizae Radix et Rhizoma by HPCE-DAD and estimate its quality. METHODS: Salviae Miltiorrhizae Radix et Rhizoma were analyzed and the chromatographic fingerprint were determined by HPCE-DAD. The data were analysed by fuzzy cluster and fingerprint similarity evaluation software was used to compare the similarity of samples. RESULTS: HPCE-DAD fingerprint of 10 main common peaks was established preliminarily. It was discovered that a small number of samples were different from the others. CONCLUSION: The method is reliable, accurate and can be used for the quality control of Salviae Miltiorrhizae Radix et Rhizoma
Asunto(s)
Medicamentos Herbarios Chinos/química , Electroforesis Capilar/métodos , Plantas Medicinales/química , Salvia miltiorrhiza/química , Cromatografía Líquida de Alta Presión/métodos , Análisis por Conglomerados , Raíces de Plantas/química , Control de Calidad , Reproducibilidad de los Resultados , Rizoma/químicaRESUMEN
In this study, laser scanning confocal microscopy (LSCM) was used to determine the location and relative quantity of flavonoids in the leaves of Apocynum venetum L. from the top, middle and basal parts of the branch. The leaves of the plants of one, two and three years old, separately, were collected in July. ANOVA and LSD test were employed in the statistical analysis. The results indicated that flavonoids located mainly in xylem conduit of vein, collenchyma, epidermic cells and cuticle. The data of flavonoids contents under statistical analysis showed that difference existed in the leaves of different parts and different ages. This study provided the reliable scientific material about the analysis of the ecological and the exploitation of the leaves of Apocynum venetom L.
Asunto(s)
Apocynum/química , Flavonoides/análisis , Hojas de la Planta/química , Plantas Medicinales/química , Microscopía Confocal , Factores de TiempoRESUMEN
OBJECTIVE: To investigate the anti-HBV effects of the extracts with alcohol and water from Arenaria kansuensis in vitro. METHODS: HepG2.2.15 cells were used to estimate the effects of the extracts with alcohol and water from Arenaria kansuensis on the levels of HBsAg and HBeAg in medium by ELISA. RESULTS: Incubation of HepG2.2.15 cells with both the extracts with alcohol and water from Arenaria kansuensis (50-400 mg/L) for 72 and 96h significantly inhibited the levels of HBsAg and HBeAg. The maximum inhibiting rate of HBeAg from the Arenaria kansuensis extracts with alcohol was 63.2% and 90. 8% at 72h and 96h, respectively. Meanwhile, the Arenaria kansuensis extracts with water had obvious inhibitory effects on the levels of HBsAg and HBeAg in HepG2.2.15 cells at 96 h, compared with that of the control group (P < 0.01). The maximum inhibiting rates were 52.5% and 72.8%, respectively. CONCLUSION: The extracts with water from Arenaria kansuensis has obvious anti-HBV effects in vitro.