RESUMEN
The rapid endothelialization of tissue-engineered blood vessels (TEBVs) can effectively prevent thrombosis and inhibit intimal hyperplasia. The traditional Chinese medicine ingredient icariin is highly promising for the treatment of cardiovascular diseases. ß-cyclodextrin sulfate is a type of hollow molecule that has good biocompatibility and anticoagulation properties and exhibits a sustained release of icariin. We studied whether icariin-loaded ß-cyclodextrin sulfate can promote the endothelialization of TEBVs. The experimental results showed that icariin could significantly promote the proliferation and migration of endothelial progenitor cells; at the same time, icariin could promote the migration of rat vascular endothelial cells (RAVECs). Subsequently, we used an electrostatic force to modify the surface of the TEBVs with icariin-loaded ß-cyclodextrin sulfate, and these vessels were implanted into the rat common carotid artery. After 3 months, micro-CT results showed that the TEBVs modified using icariin-loaded ß-cyclodextrin sulfate had a greater patency rate. Scanning electron microscopy (SEM) and CD31 immunofluorescence results showed a better degree of endothelialization. Taken together, icariin-loaded ß-cyclodextrin sulfate can achieve anticoagulation and rapid endothelialization of TEBVs to ensure their long-term patency.
Asunto(s)
Coagulación Sanguínea/efectos de los fármacos , Vasos Sanguíneos/efectos de los fármacos , Células Progenitoras Endoteliales/efectos de los fármacos , Flavonoides/farmacología , beta-Ciclodextrinas/farmacología , Animales , Prótesis Vascular , Vasos Sanguíneos/metabolismo , Vasos Sanguíneos/fisiología , Movimiento Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Células Progenitoras Endoteliales/metabolismo , Células Progenitoras Endoteliales/fisiología , Flavonoides/química , Ratas Sprague-Dawley , Sulfatos/metabolismo , Ingeniería de Tejidos/métodos , beta-Ciclodextrinas/química , beta-Ciclodextrinas/metabolismoRESUMEN
Regulation of cellular response pattern to phosphorus ion (PI) is a new target for the design of tissue-engineered materials. Changing cellular response pattern to high PI can maintain monocyte/macrophage survival in TEBV and the signal of increasing PI can be converted by klotho to the adenosine signals through the regulation of energy metabolism in monocytes/macrophages.
Asunto(s)
Vasos Sanguíneos/efectos de los fármacos , Vasos Sanguíneos/fisiología , Fósforo/farmacología , Animales , Macrófagos/efectos de los fármacos , Macrófagos/fisiología , Monocitos/efectos de los fármacos , Monocitos/fisiología , Ratas , Ratas Wistar , Ingeniería de Tejidos/métodosRESUMEN
NiO-nanoflakes (NiO-NFs) grafted Ni-nanorod (Ni-NR) arrays stuck out of the porous anodic aluminum oxide (AAO) template are achieved by a combinatorial process of AAO-confined electrodeposition of Ni-NRs, selectively etching part of the AAO template to expose the Ni-NRs, wet-etching the exposed Ni-NRs in ammonia to obtain Ni(OH)2-NFs grafted onto the cone-shaped Ni-NRs, and annealing to transform Ni(OH)2-NFs in situ into NiO-NFs. By top-view sputtering, Ag-nanoparticles (Ag-NPs) are decorated on each NiO-NFs grafted Ni-NR (denoted as NiO-NFs@Ni-NR). The resultant Ag-NPs-decorated NiO-NFs@Ni-NR (denoted as Ag-NPs@NiO-NFs@Ni-NR) arrays exhibit not only strong surface-enhanced Raman scattering (SERS) activity but also reproducible SERS-signals over the whole array. It is demonstrated that the strong SERS-activity is mainly ascribed to the high density of sub-10 nm gaps (hot spots) between the neighboring Ag-NPs, the semiconducting NiO-NFs induced chemical enhancement effect, and the lightning rod effect of the cone-shaped Ni-NRs. The three-level hierarchical nanostructure arrays stuck out of the AAO template can be utilized to probe polychlorinated biphenyls (PCBs, a kind of global environmental hazard) with a concentration as low as 5 × 10(-6) M, showing promising potential in SERS-based rapid detection of organic environmental pollutants.
Asunto(s)
Óxido de Aluminio/química , Nanopartículas del Metal/química , Nanotubos/química , Níquel/química , Plata/química , Nanotubos/ultraestructura , Bifenilos Policlorados/análisis , Porosidad , Rodaminas/química , Espectrometría RamanRESUMEN
A highly sensitive and selective fluorescence ratiometric sensor membrane for 2,3,3'-trichlorobiphenyl has been achieved, via depositing polypyrrole nanotubes (PPyNTs, the fluorescence indicator) in nano-porous anodic aluminium oxide (NPAAO) template and subsequently immobilizing fluorescein isothiocyanate (as an internal reference) onto the inner walls of the PPyNTs embedded in the NPAAO.
Asunto(s)
Óxido de Aluminio/química , Fluoresceína-5-Isotiocianato/química , Membranas Artificiales , Nanotubos/química , Bifenilos Policlorados/aislamiento & purificación , Polímeros/química , Pirroles/química , Espectrometría de Fluorescencia/instrumentación , Colorantes Fluorescentes , Nanotubos/ultraestructura , Sensibilidad y EspecificidadRESUMEN
Ag-capped Au nanopillar arrays on a resin supporter (see left upper figure), with a typical adjacent pillar tip gap of 10 nm, show obviously higher surface-enhanced Raman scattering (SERS) sensitivity (right column in red) than that of the bare Au nanopillar array while using 10 nM R6G as probe molecules. The large-area Ag-capped Au nanopillar array has potential in trace detection of special chemicals.
Asunto(s)
Oro/química , Nanopartículas del Metal/química , Plata/química , Espectrometría Raman/métodos , Óxido de Aluminio/química , Nanopartículas del Metal/ultraestructura , Bifenilos Policlorados/análisisRESUMEN
Generic approaches to nano-dots and nano-rings have been developed via sputtering an Au layer on one planar surface side of porous anodic aluminum oxide template and subsequent electrodeposition.