RESUMEN
OBJECTIVE: To compare Cirsium japonicum characteristics with C. leo and C. leducei, along with the content of buddleoside and pectolinarin, and lay the foundation for the quality control of C. japonicum. METHOD: Samples were collected and the relevant drugs were bought. The samples were divided into root, stem, leaf and flower, and the content of buddleoside and pectolinarin was determine by the HPLC. Chromatographic column: Waters XBridge C18 (4.6 mm x 250 mm), mobile phase: methanol-water (45: 55), measurement wavelength: 326 nm, flow rate: 0.8 mL x min(-1), column temperature: 30 degrees C. RESULT AND CONDUSION: Standard curve equation of buddleoside: Y = 74 064X-47 748, R2 = 0.991. Standard curve equation of pectolinarin: Y = 1 711 64X - 180 707, R2 = 0.999. The content of buddleoside: C. japonicum leaf was 1.987 3%, C. leo leaf 1.412 2%, C. leducei leaf 0.149 2%. The content of buddleoside was lower in root and stem. Pectolinarin was not detected in the C. japonicum and C. leo. The pectolinarin content was 0.069 0% in C. leducei leaf.
Asunto(s)
Cromonas/análisis , Cirsium/química , Medicamentos Herbarios Chinos/análisis , Cromatografía Líquida de Alta Presión , Cromonas/química , Medicamentos Herbarios Chinos/química , Reproducibilidad de los Resultados , Solubilidad , Especificidad de la EspecieRESUMEN
Three new phenolic compounds, eurycorymboside A (1), eurycorymboside B (6), and eurycorymbic acid (8), were isolated from the stem part of Eurycorymbus cavaleriei (Sapindaceae) along with five known phenolic compounds, glucosyringic acid (2), vanillic acid 4-O-ß-d-glucoside (3), koaburaside (4), tachioside (5), and 4-hydroxy-3,5-bis(3-methyl-2-butenyl)benzaldehyde (7). The structures were established on the basis of spectral analysis. The antioxidant activities of compounds 1-6 were evaluated by the 1,1-diphenyl-2-picrylhydrazyl-free radical scavenging assay. Compound 4 exhibited antioxidant activity with an IC(50) value of 9.0 µM. Compound 4 also showed weak inhibitory activity against influenza A neuraminidase.
Asunto(s)
Antioxidantes/aislamiento & purificación , Antivirales/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Glicósidos/aislamiento & purificación , Fenoles/aislamiento & purificación , Sapindaceae/química , Antioxidantes/química , Antioxidantes/farmacología , Antivirales/química , Antivirales/farmacología , Compuestos de Bifenilo/farmacología , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Glicósidos/química , Glicósidos/farmacología , Virus de la Influenza A/efectos de los fármacos , Virus de la Influenza A/enzimología , Neuraminidasa/antagonistas & inhibidores , Fenoles/química , Fenoles/farmacología , Picratos/farmacología , Tallos de la Planta/químicaRESUMEN
The chemical constituents of Rhodiola kirilowii were separated and purified by repeated column chromatography on silica gel, RP - 18, Sephadex LH -20 and semi-preparative HPLC. Each compound was characterized by spectroscopic and physical data. Twelve compounds have been purified and identified to be beta-sitosterol (1), tyrosol (2), trans-hydroxycinnamic acid (3), geranyl beta-glucopyranoside (4), neryl beta-glucopyranoside (5), hexyl beta-glucopyranoside (6), gallic acid (7), (-) -epigallocatechin gallate (8), rhodiolgin (9), isolariciresinol-9-O-beta-glucopyranoside (10), rhodiooctanoside (11), and sacranoside B (12). Among them, compounds 3, 6, 9-12 were isolated from Rhodiola kirilowii for the first time; Compounds 4 and 5 were obtained for the first time from the genus Rhodiola. The in vitro activities against Macobacterium tuberculosis (ATCC 27294) of its extracts and pure components were evaluated by testing their MIC (minimal inhibitory concentration) and MBC (minimal bactericidal concentration). The 80% (a. q.) EtOH extract, EtOAc-soluble extract, compounds 7 and 8 exhibited in-vitro inhibitory and bactericidal activities against Macobacterium tuberculosis in different extent.
Asunto(s)
Antituberculosos/aislamiento & purificación , Antituberculosos/farmacología , Raíces de Plantas/química , Rhodiola/química , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Pruebas de Sensibilidad Microbiana , Mycobacterium tuberculosis/efectos de los fármacosRESUMEN
OBJECTIVE: This research focused on analyzing the differences of 5S rRNA gene spacer sequences on Swertia mussotii and its commonly used adulterants, including S. franchetiana, S. wolfangiana and S. chirayita. METHOD: DNA was extracted from the collected Swertia samples. 5S rRNA intergenic spacers were amplified by PCR, sequenced and analyzed. RESULT: 5S rRNA gene spacer sequences were different between S. mussotii and its other three adulterants. Sequence divergence among species ranged from 30.6% to 65.0%. CONCLUSION: 5S rRNA spacers may be used as molecular authentication markers to differentiate S. mussotii and other commonly used Swertia adulterants. This result provides reliable and simple reference for the authentication of Swertia genus species.
Asunto(s)
ARN Ribosómico 5S/genética , Swertia/clasificación , Swertia/genética , Secuencia de Bases , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Homología de Secuencia de Ácido NucleicoRESUMEN
OBJECTIVE: To differentiate and identify several species of "Muxiang". METHODS: The main microscopic features and HPLC chromatograms of diagnostic components were studied. RESULTS: Distinguishable characters were found in two methods. CONCLUSION: The results provide reliable and simple reference for the authentication of raw materials of" Muxiang" species, especially the toxic species.
Asunto(s)
Asteraceae/ultraestructura , Cromatografía Líquida de Alta Presión/métodos , Plantas Medicinales/ultraestructura , Ácidos Aristolóquicos/análisis , Asteraceae/química , Asteraceae/clasificación , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/normas , Lactonas/análisis , Raíces de Plantas/química , Raíces de Plantas/ultraestructura , Plantas Medicinales/química , Control de Calidad , Reproducibilidad de los ResultadosRESUMEN
OBJECTIVE: To determine the contents of morusin in Sangbaipi (Cortex Mori) of different commercial grades and species and obtained from different regions. METHOD: Contents of morusin were determined by HPLC on an Alltima C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water (58:42) as mobile phase. UV detection was conducted at 270 nm. RESULT: The amounts of morusin were found to vary among samples of Cortex Mori bearing a yellow-brown outer layer and those without the yellow-brown cork layer. The morusin contents were also related to the thickness of Cortex Mori. CONCLUSION: The method was simple, reproducible and reliable. It can be applied to the quality assessment of Cortex Mori.
Asunto(s)
Medicamentos Herbarios Chinos/análisis , Flavonoides/análisis , Morus/química , Raíces de Plantas/química , China , Cromatografía Líquida de Alta Presión , Medicamentos Herbarios Chinos/normas , Ecosistema , Plantas Medicinales/química , Control de Calidad , Reproducibilidad de los Resultados , Espectrofotometría UltravioletaRESUMEN
OBJECTIVE: To dertermine SO2 in Chinese herbal medicine with both food and medicinal functions. METHODS: A distilling and ion-chromatorgraphic method was applied. RESULTS: The results indicated that among 32 commonly used Chinese herbal medicines, 26 samples contained sulphur dioxide, varying in its contents and up to 3180 microg/g. The amount of sulphur dioxide did not change much after samples were washed by water. On the other hand, sulphur dioxide contents decreased after samples were exposed outdoor or boiled in water. CONCLUSION: The method was simple, accurate and reproducible.